Potensi dan Aktivitas Enzimatik Isolat Yeast W7.3 & TPSL terhadap Degradasi Phenanthrene

Fitriya, Salsabila Aida (2023) Potensi dan Aktivitas Enzimatik Isolat Yeast W7.3 & TPSL terhadap Degradasi Phenanthrene. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Pencemaran lingkungan oleh hidrokarbon diesel fuel masih menjadi sorotan global. Diesel fuel didominasi oleh komponen polycyclic aromatic hydrocarbon (PAH) sebanyak 95%-99% yang bersifat toksik, karsiogenik, mutagenik, hidrofobisitas tinggi, dan volatilitas yang rendah. Komponen mayoritas dalam PAH diesel fuel adalah phenanthrene (PHE). Salah satu solusi dalam penanganan cemaran PAH yakni biodegradasi dengan memanfaatkan potensi metabolisme mikroorganisme untuk mendegradasi dan mengubah senyawa PAH menjadi senyawa tidak beracun. Yeast merupakan mikroorganisme eukariotik yang memiliki metabolisme enzimatik untuk mereduksi senyawa hidrokarbon dan memanfaatkannya sebagai sumber karbon serta dapat mengoksidasi komponen PAH dalam jalur metabolisme maupun kometabolisme. Degradasi tersebut tidak terlepas dari peran enzim yang diekspresikan oleh yeast. Enzim yang berperan dalam degradasi komponen PAH yakni dalam golongan oksidoreduktase diantaranya yakni laccase dan manganese peroxidase. Pada penelitian kali ini digunakan senyawa spesifik berupa PHE dari komponen PAH pada diesel fuel. Aktivitas oksidoreduktase diukur secara spektrofotometri pada hari ke-30 waktu inkubasi menggunakan syringaldazine. Kadar PHE dideteksi secara kolorimetri menggunakan nanopartikel emas (AuNPs) pada hari ke 0, 2, 5, 10, 15, 20, 25, dan 30 waktu inkubasi. Pada waktu akhir inkubasi dilakukan analisis High Performance Liquid Chromatography (HPLC) untuk mengkonfirmasi konsentrasi PHE. Analisis data hasil penelitian dilakukan secara kuantitatif menggunakan pengujian Two Way ANOVA untuk melihat pengaruh jenis isolat dan waktu inkubasi terhadap degradasi PHE. Hasil dari penelitian menunjukkan terdapat aktivitas oksidoreduktase sebesar 0,664 U/ml untuk isolat W7.3 dan 0,385 U/ml untuk isolat TPSL pada hari ke-30. Waktu inkubasi berpengaruh terhadap degradasi PHE, ditunjukkan dengan kadar PHE yang mengalami tren penurunan yang signifikan (p value <0,05) seiring dengan bertambahnya waktu inkubasi. Hasil analisis HPLC menunjukkan bahwa efisiensi degradasi phenanthrene oleh isolat W7.3 sebesar 74,33% dan isolat TPSL sebesar 31,68%.
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Environmental pollution by diesel fuel hydrocarbons is still a global concern. Diesel fuel is dominated by 95% -99% polycyclic aromatic hydrocarbon (PAH) components which are toxic, carcinogenic, mutagenic, high hydrophobicity and low volatility. The majority component in diesel fuel PAHs is phenanthrene (PHE). One of the solutions in handling PAH contamination is biodegradation by utilizing the metabolic potential of microorganisms to degrade and convert PAH compounds into non-toxic compounds. Yeast is a eukaryotic microorganism that has an enzymatic metabolism to reduce hydrocarbon compounds and utilize them as a carbon source and can oxidize PAH components in metabolic and co-metabolic pathways. This degradation is inseparable from the role of enzymes expressed by yeast. The enzymes that play a role in the degradation of PAH components are in the oxidoreductase class including laccase and manganese peroxidase. In this study, a specific compound in the form of PHE was used from the PAH component in diesel fuel. Oxidoreductase activity was measured spectrophotometrically on the 30th day of incubation using syringaldazine. PHE levels were detected colorimetrically using gold nanoparticles (AuNPs) on days 0, 2, 5, 10, 15, 20, 25, and 30 of incubation time. At the end of incubation, High Performance Liquid Chromatography (HPLC) analysis was carried out to confirm the concentration of PHE. Analysis of the research data was carried out quantitatively using the Two Way ANOVA test to see the effect of isolate type and incubation time on PHE degradation. The results of the study showed that there was oxidoreductase activity of 0.664 U/ml for W7.3 isolate and 0.385 U/ml for TPSL isolate on the 30th day. Incubation time had an effect on PHE degradation, as indicated by PHE levels which experienced a significant decreasing trend (p value< 0.05) as the incubation time increased. The results of the HPLC analysis showed that the efficiency of phenanthrene degradation by isolate W7.3 was 74.33% and by isolate TPSL was 31.68%.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Diesel fuel, Hidrokarbon, Oksidoreduktase, PAH, Phenanthrene, Yeast, Hydrocarbon, Oxidoreductase.
Subjects:	Q Science > QH Biology > QH301 Biology Q Science > QR Microbiology
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Salsabila Aida Fitriya
Date Deposited:	02 Aug 2023 08:20
Last Modified:	02 Aug 2023 08:20
URI:	http://repository.its.ac.id/id/eprint/101862

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