Optimasi Sintesis Nanopartikel Perak dengan Response Surface Methodology dan Studi Aktivitas Anti-bakterinya

Adibah, Nisrina (2023) Optimasi Sintesis Nanopartikel Perak dengan Response Surface Methodology dan Studi Aktivitas Anti-bakterinya. Masters thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Sintesis nanopartikel perak dipengaruhi oleh beberapa parameter sehingga optimasi selalu dilakukan untuk mendapatkannya. Metode one factor at a time (OFAT) yang tidak dapat melihat interaksi lebih dari dua faktor seringkali digunakan untuk mengoptimasi sintesis nanopartikel perak. Pada penelitian ini digunakan desain eksperimen response surface methodology (RSM) berjenis faced-centered central composite design (FCCD) untuk mendapatkan wilayah optimum sintesis nanopartikel perak dengan metode reduksi kimia. Optimasi sintesis nanopartikel perak meliputi tiga variabel, yaitu konsentrasi prekursor (perak nitrat), konsentrasi reduktor (asam askorbat) dan waktu pengadukan melalui perangkat lunak Python. Hasil optimum sintesis nanopartikel perak dievaluasi aktivitas anti-bakterinya terhadap bakteri Eschericia coli (bakteri gram negatif) dan Staphylococcus aureus (bakteri gram positif) serta dikarakterisasi dengan particle size analyzer (PSA) dan transmission electron microscopy(TEM). Hasil optimasi menunjukkan bahwa tiga variabel yang diteliti signifikan terhadap respons secara statistik dengan dieperolehnya nilai F-value sebesar 31,97 dan P-value sebesar 0,00068. Wilayah dan kondisi optimum sintesis nanopartikel perak dapat diamati dari parameter dan rentang percobaan yang dilakukan yaitu konsentrasi prekursor sebesar 0,005-0,020 M, konsentrasi reduktor sebesar 0,005-0,050 M dan waktu pengadukan sebesar 0-20 menit. Nanopartikel perak yang teroptimasi berbentuk spherical dan memiliki aktivitas anti-bakteri terhadap kedua bakteri yang digunakan.
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Silver nanoparticle synthesis is influenced by several parameters, so optimization is always carried out to achieve the desired results. The one factor at a time (OFAT) method, which can not capture interactions among more than two factors, is often used to optimize silver nanoparticle synthesis. In this study, a response surface methodology (RSM) experimental design of the faced-centered central composite design (FCCD) type was employed to obtain the optimum region silver nanoparticle synthesis. The optimization of silver nanoparticle synthesis involved three variables: precursor concentration (silver nitrate), reducing agent concentration (ascorbic acid) and stirring time, implemented through Python application. The optimum results of silver nanoparticle synthesis were evaluated for their antibacterial activity against Escherichia coli (gram-negative bacteria) and Staphylococcus aureus (gram-positive bacteria), and characterized using a particle size analyzer (PSA) as well as transmission electron microscopy (TEM). The optimization results indicate that the three investigated variables are statistically significant in relation to the response, with an obtained F-value of 31,97 and a P-value of 0,00068. The optimum region and conditions for silver nanoparticle synthesis can be observed from the variables and experimental ranges conducted, namely precursor concentrations of 0,005-0,020 M, reductant concentrations of 0,005-0,050 M and stirring time of 0-20 minutes. The optimized silver nanoparticles have a spherical shape and exhibit antibacterial activity against both bacteria used.

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	response surface methodology, central composite design, nanopartikel perak, anti-bakteri; response surface methodology, central composite design, silver nanoparticles, anti-bacterial
Subjects:	Q Science > QD Chemistry > QD75.2 Chemistry, Analytic
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47101-(S2) Master Thesis
Depositing User:	Nisrina Adibah
Date Deposited:	31 Aug 2023 03:20
Last Modified:	31 Aug 2023 03:20
URI:	http://repository.its.ac.id/id/eprint/101876

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