Uji Degradasi Komponen Polisakarida oleh Konsorsium Bakteri Dekomposer

Ramadhani, Muhammad Alif (2023) Uji Degradasi Komponen Polisakarida oleh Konsorsium Bakteri Dekomposer. Other thesis, Insititut Teknologi Sepuluh Nopember.

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Abstract

Biodekomposer merupakan mikroorganisme yang dapat menguraikan substrat menjadi bentuk yang lebih sederhana. Limbah Tandan Kosong Kelapa Sawit (TKKS) mengandung 33,25% selulosa; 23,24% hemiselulosa; 25,38 lignin; 8,56% air; serta 4,19% zat ekstraktif. Bakteri selulolitik dan ligninolitik berpotensi untuk mendegradasi limbah TKKS. dengan enzim selulase, lignin peroksidase (LiP), mangan peroksidase (MnP) dan lakase (LaC). Penelitian ini bertujuan untuk mengisolasi bakteri Lactobacillus, Bacillus, Pseudomonas dan Acetobacter dari konsorsium bioaktivator dan mengetahui potensinya dalam menghasilkan enzim selulase dan ligninase. Isolasi dilakukan dengan metode standar dan dikarakterisasi biokimianya mengikuti Bergey’s Determinative Bacteria untuk mengetahui genusnya. Isolat bakteri yang ditemukan sejumlah 17 isolat dengan dua isolat yaitu B5-1 dan B6-3 yang memiliki karakter mendekati Bacillus dan isolat B5-3 mendekati Pseudomonas. Bakteri target dari genus Lactobacillus dan Acetobacter tidak ditemukan. Deteksi enzimatik dilakukan secara kualitatif dengan uji zona bening dan perubahan warna pada medium uji. Aktivitas selulase tertinggi ditunjukkan oleh isolat B1-5 dengan nilai Indeks Aktivitas Degradasi (IAD) 4,57 dan B5-1 dengan nilai IAD sebesar 4,64. Aktivitas LiP hanya ditunjukkan oleh isolat B6-3 dengan nilai IAD sebesar 0,81. Aktivitas LaC hanya ditemukan pada isolat B2-3 dan B4-3, serta tidak satu pun isolat bakteri memiliki aktivitas MnP.
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Biodecomposers are microorganisms that can decompose substrates into simpler forms. Oil Palm Empty Bunches (EFB) contains 33.25% cellulose; 23.24% hemicellulose; 25.38 lignins; 8.56% water; and 4.19% extractive substances. Cellulolytic and ligninolytic bacteria have the potential to degrade OPEFB waste. with cellulase enzymes, lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase (LaC). This study aims to isolate Lactobacillus, Bacillus, Pseudomonas and Acetobacter bacteria from a bioactivator consortium and determine their potential to produce cellulase and ligninase enzymes. Isolation was carried out using standard methods and biochemical characterization following Bergey's Determinative Bacteria to determine the genus. Bacterial isolates found were 17 isolates with two isolates, namely B5-1 and B6-3 which had characters close to Bacillus and isolate B5-3 close to Pseudomonas. Target bacteria from the genus Lactobacillus and Acetobacter were not found. Enzymatic detection was carried out qualitatively by testing the clear zone and changing the color of the test medium. The highest cellulase activity was shown by isolates B1-5 with a Degradation Activity Index (IAD) value of 4.57 and B5-1 with an IAD value of 4.64. LiP activity was only shown by isolate B6-3 with an IAD value of 0.81. LaC activity was only found in isolates B2-3 and B4-3, and none of the bacterial isolates had MnP activity.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Bioaktivator, Ligninolitik, Selulolitik, Zona Bening; Bioactivator, Cellulolytic, Clear Zone, Lignolytic
Subjects:	Q Science > QR Microbiology Q Science > QR Microbiology > QR74.8 Bacteria
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Muhammad Alif Ramadhani
Date Deposited:	03 Aug 2023 15:25
Last Modified:	29 Aug 2023 03:19
URI:	http://repository.its.ac.id/id/eprint/101898

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