Produksi Dan Karakterisasi Kitinase Dari Bakteri Limbah Perikanan Dan Aplikasinya Dalam Penghambatan Pertumbuhan Jamur Penyusun Lumut Kerak

Sagita, Sari Ayu (2023) Produksi Dan Karakterisasi Kitinase Dari Bakteri Limbah Perikanan Dan Aplikasinya Dalam Penghambatan Pertumbuhan Jamur Penyusun Lumut Kerak. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Pelestarian candi memiliki beberapa tantangan salah satunya pelapukan batuan yang diakibatkan oleh organisme seperti lumut kerak atau lichenes. Upaya penghambatan pertumbuhan jamur yang ramah lingkungan salah satunya dengan enzim yang mampu mendegradasi kitin yaitu kitinase dapat diekstrak dari bakteri kitinolitik yang biasanya melimpah di limbah perikanan. Kitinase sering dimanfaatkan dalam bidang pertanian sebagai agen biokontrol terhadap fungi patogen tetapi potensinya dalam menghambat pertumbuhan jamur perusak batuan candi belum pernah dilakukan. Tujuan dari penelitian ini adalah mengetahui aktivitas kitinase dari isolat bakteri kitinolitik limbah perikanan dan potensinya dalam menghambat pertumbuhan jamur penyusun lumut kerak. Crude enzim diekstrak dari bakteri kitinolitik, dimurnikan parsial dengan presiptasi ammonium sulfat. Karakterisasi enzim dilakukan dengan pengukuran aktivitas dengan metode DNS dan konsentrasi total protein dengan metode Bradford pada tiap tahap presipitasi. Presipitasi tertinggi akan dilakukan penentuan titik isoelektrik. Uji kemampuan kitinase dalam menghambat pertumbuhan jamur menggunakan media cair, parameter penghambatan pertumbuhan jamur oleh kitinase ialah biomassa jamur. Penelitian menggunakan Rancangan Acak Lengkap (RAL), analisis data secara deskriptif kuantitatif dan kualitatif. Isolat bakteri kitinolitik yang berhasil diisolasi dan dimurnikan dari limbah perikanan berjumlah 23 isolat, secara makroskopis didominasi dengan koloni berwarna putih, transparan, dan putih transparan dan berbentuk circular. Karakteristik morfologi secara mikroskopis didominasi oleh bakteri Gram negatif dan berbentuk antara coccus dan bacil. Kitinasi dari isolat K8 memiliki indeks kitinolitik terbesar. Crude kitinase dengan aktivitas 113 U/mL. Pada pemurnian parsial menggunakan ammonium sulfat, kitinase mempunyai aktivitas spesifik sebesar 1.078,14 U/mg. Kitinase dari isolat K8 mampu menghambat pertumbuhan jamur Purpureocillium lilacinum dengan penurunan biomassa mencapai 72,8% menjadi 0,3 mg/mL di hari ke-7 pemaparan dari 1,1 mg/mL menjadi 0,3 mg/mL
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The preservation of the temple has several challenges, one of which is the weathering of rocks caused by organisms such as lichens or lichens. One of the efforts to inhibit the growth of environmentally friendly fungi is by means of an enzyme capable of degrading chitin, namely chitinase which can be extracted from chitinolytic bacteria which are usually abundant in fishery waste. Chitinase is often used in agriculture as a biocontrol agent against fungal pathogens, but its potential to inhibit the growth of fungi destroying temple rocks has never been tested. The purpose of this study was to determine the activity of chitinase from chitinolytic bacteria isolates from fishery waste and its potential to inhibit the growth of the lichen-forming fungi. Enzyme crude extracted from chitinolytic bacteria, partially purified by ammonium sulfate precipitation. Enzyme characterization was carried out by measuring activity with the DNS method and total protein concentration with the Bradford method at each precipitation stage. The highest precipitation will be determined by the isoelectric point. Test the ability of chitinase to inhibit fungal growth using liquid media, the parameter of inhibition of fungal growth by chitinase is the fungal biomass. The study used a completely randomized design (CRD), data analysis was descriptive quantitative and qualitative. Chitinolytic bacteria isolates that were successfully isolated and purified from fishery waste totaled 23 isolates, macroscopically dominated by white, transparent, and white transparent colonies and circular shapes. Microscopic morphological characteristics are dominated by Gram negative bacteria and are shaped between coccus and bacillus. The chitination of isolate K8 has the largest chitinolytic index. Crude chitinase with an activity of 113 U/mL. In partial purification using ammonium sulfate, chitinase has a specific activity of 1,078.14 U/mg. Chitinase from isolate K8 was able to inhibit the growth of the fungus Purpureocillium lilacinum with a reduction in biomass reaching 72.8% to 0.3 mg/mL on the 7th day of exposure from 1.1 mg/mL to 0.3 mg/mL

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Bakteri kitinolitik, Jamur, Kitinase, Limbah perikanan, Lumut kerak; Chitinolytic bacteria, Chitinase, Fishery waste, Fungi, Lichens
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Sari Ayu Sagita
Date Deposited:	05 Sep 2023 03:39
Last Modified:	05 Sep 2023 03:39
URI:	http://repository.its.ac.id/id/eprint/102594

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