Kuantifikasi dan Identifikasi Bakteri Resisten Vankomisin Pada Usus Manusia

Andini, Andini (2023) Kuantifikasi dan Identifikasi Bakteri Resisten Vankomisin Pada Usus Manusia. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Vankomisin merupakan salah satu antibiotik yang dapat menangani kasus Methicillin-Resistant Staphylococcus aureus (MRSA) di tahun 1960-an. Penggunaan vankomisin dalam spektrum luas disertai pengguaan dosis yang tidak tepat mengakibatkan munculnya kasus resisten vankomisin di berbagai negara salah satunya di Indonesia. Sampai saat ini belum ada laporan atau penelitian mengenai persebaran strain resisten vankomisin yang ditemukan pada usus manusia di Jawa Timur. Penelitian ini bertujuan untuk mengetahui prevalensi, jumlah sel bakteri, dan spesies bakteri resisten vankomisin yang ditemukan pada usus manusia di Jawa Timur. Sampel feses dikumpulkan dari 22 partisipan yang memenuhi kriteria dan dilakukan enumerasi bakteri dengan metode (TPC) menggunakan media TSA + vankomisin dengan konsentrasi 32 μg/ml. Koloni yang tumbuh kemudian diidentifikasi berdasarkan sekuens 16S rRNA. Didapatkan hasil prevalensi bakteri resisten vankomisin yang terdeteksi di usus manusia populasi Kenjeran dan Pacet sebesar 63,3% dan 100% dengan rata - rata densitas tertinggi 45,5 x 105 CFU/gram dan terendah adalah 105 CFU/gram. Hasil identifikasi spesies bakteri resisten vankomisin yang ditemukan di usus populasi Kenjeran meliputi E. fergusonii, P. stutzeri, E. hormaechei, dan S. flexneri. Sedangkan di usus populasi Pacet meliputi W. confusa, P. stutzeri, S. boydii, L. plantarum, E. fergusonii, A. baumannii, P. aeruginosa, C. mobilis, K. pneumoniae, S. flexneri, dan S. limneticum.
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Vancomycin is one of the antibiotics that can treat cases of Methicillin-Resistant Staphylococcus aureus (MRSA) in the 1960s. The use of vancomycin in a broad spectrum accompanied by the use of inappropriate doses has resulted in the emergence of vancomycin-resistant cases in various countries, one of which is in Indonesia. Until now there have been no reports or studies regarding the distribution of vancomycin-resistant strains found in the human intestine in East Java. This study aims to determine the prevalence, number of bacterial cells, and species of vancomycin-resistant bacteria found in the human intestine in East Java. Stool samples were collected from 22 participants who met the criteria and enumeration of bacteria was carried out using the TSA + vancomycin media with a concentration of 32 μg/ml. The growing colonies were then identified based on the 16S rRNA sequence. The prevalence of vancomycin-resistant bacteria detected in the human intestine in the Kenjeran and Pacet populations was 63,3% and 100% with the highest average density being 45,5 x 105 CFU/gram and the lowest being 105 CFU/gram. The results of the identification of vancomycin-resistant bacterial species found in the intestines of the Kenjeran population included E. fergusonii, P. stutzeri, E. hormaechei, and S. flexneri. Whereas in the intestines the Pacet population includes W. confusa, P. stutzeri, S. boydii, L. plantarum, E. fergusonii, A. baumannii, P. aeruginosa, C. mobilis, K. pneumoniae, S. flexneri, and S. limneticum .

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Antibiotik, Feses Manusia, Gen 16S rRNA, Vankomisin; Antibiotics, 16S rRNA Gene, Human Feces, Vancomycin
Subjects:	Q Science > QH Biology Q Science > QH Biology > QH301 Biology
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Andini Andini
Date Deposited:	04 Oct 2023 01:48
Last Modified:	04 Oct 2023 01:48
URI:	http://repository.its.ac.id/id/eprint/103089

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