Respons Morfo-fisiologi dan Molekuler Tomat Mutan iaa9-3 dan iaa9-5 Kultivar Micro-Tom sebagai Kandidat Tomat Heat Tolerant

Rafsyanyani, Cory (2023) Respons Morfo-fisiologi dan Molekuler Tomat Mutan iaa9-3 dan iaa9-5 Kultivar Micro-Tom sebagai Kandidat Tomat Heat Tolerant. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Peningkatan suhu bumi sebagai dampak pemanasan global mengancam tercapainya target peningkatan produksi komoditas pertanian, termasuk tomat (Solanum lycopersicum L.). Auksin merupakan salah satu fitohormon yang berperan penting dalam mekanisme ketahanan tanaman terhadap heat stress. Gen IAA9 mengkode protein AUXIN/INDOLE-3-ACETIC-ACID (Aux/IAA) yang berfungsi dalam mekanisme regulasi auksin. Meskipun demikian, informasi mengenai mekanisme regulasi gen tersebut dalam mekanisme respons tanaman terhadap heat stress masih terbatas. Oleh karena itu, penelitian ini bertujuan untuk mengetahui peranan gen IAA9 dalam meregulasi respons tanaman tomat terhadap heat stress. Penelitian ini dilakukan menggunakan studi tomat mutan iaa9 (iaa9-3 dan iaa9-5) yang diberi perlakuan heat stress pada rentang suhu 40-45⁰C untuk pengamatan respons morfo-fisiologi dan 38-40⁰C untuk pengamatan respons molekuler berupa analisis ekspresi gen SlDREBA4. Beberapa parameter thermo-morphogenesis (bentuk daun, luas daun, jumlah daun, tinggi tanaman) dan parameter fisiologi (kandungan klorofil) dianalisis menggunakan Student’s T-Test pada P-Value < 0,05. Selain itu, pengamatan respons molekuler berupa analisis ekspresi gen SlDREBA4 juga diamati dan dianalisis secara deskriptif menggunakan Real-Time quantitative Reverse Transcriptase PCR (Real-Time qRT-PCR). Hasil yang didapatkan menunjukkan bahwa perlakuan heat stress pada suhu 40-45°C selama 6 minggu menyebabkan penurunan luas daun, jumlah daun, dan jumlah klorofil total serta peningkatan tinggi tanaman pada Wild-Type Micro-Tom (WT-MT) serta mutan iaa9-3 dan iaa9-5 dibandingkan dengan perlakuan suhu normal. Luas daun, jumlah daun, dan jumlah klorofil total terendah terdapat pada WT-MT masing-masing sebesar 165,89 cm2, 23 daun, 115,7 µg/g, sedangkan tinggi tanaman tertinggi terdapat pada mutan iaa9-3 dan iaa9-5 masing-masing sebesar 11,98 cm dan 12,13 cm, menunjukkan bahwa mutan iaa9-3 dan iaa9-5 lebih toleran terhadap heat stress dibandingkan dengan WT-MT. Lebih lanjut, perlakuan heat stress pada suhu 38-40°C selama 6 hari menyebabkan peningkatan tingkat ekspresi mRNA relatif gen SlDREBA4 pada mutan iaa9-3 dan iaa9-5 dibandingkan dengan perlakuan suhu normal masing-masing sebesar 0,45 fold dan 1,78 fold.
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The increase in earth temperature due to global warming threatens the target of increasing agricultural commodity production, including tomato (Solanum lycopersicum L.). Auxin is one of the phytohormones that plays an important role in the mechanism of plant resistance to heat stress. The IAA9 gene encodes the protein AUXIN/INDOLE-3-ACETIC-ACID (Aux/IAA) which is involved in the mechanism of auxin regulation. However, information on the regulation mechanism of this gene in the mechanism of plant response to heat stress is still limited. Therefore, this study aims to determine the role of IAA9 gene in regulating the response of tomato plants to heat stress. This study was conducted using iaa9 mutant tomatoes (iaa9-3 and iaa9-5) that were treated with heat stress at 40-45⁰C for observation of morpho-physiological responses and 38-40⁰C for observation of molecular responses by analyzing SlDREBA4 gene expression. Several thermo-morphogenesis parameters (leaf shape, leaf area, number of leaves, plant height) and physiological parameters (leaf chlorophyll content) were analyzed using Student's T-Test at P-Value < 0,05. Moreover, molecular response observation by analyzing SlDREBA4 gene expression was also observed and analyzed descriptively using Real-Time quantitative Reverse Transcriptase PCR (Real-Time qRT-PCR). The results showed that heat stress at 40-45°C for 6 weeks caused a decrease in leaf area, number of leaves, and total leaf chlorophyll content and an increase in plant height in Wild-Type Micro-Tom (WT-MT), iaa9-3, and iaa9-5 compared to normal condition. The lowest leaf area, number of leaves, and total leaf chlorophyll content occurred in WT-MT at 165,89 cm2, 23 leaves, 115,7 µg/g, respectively, while the highest plant height occurred in iaa9-3 and iaa9-5 mutants at 11,98 cm and 12,13 cm, respectively, which indicated that iaa9-3 and iaa9-5 mutants are more tolerant to heat stress compared to WT-MT. Furthermore, heat stress at 38-40°C for 6 days caused an increase in the relative mRNA expression level of the SlDREBA4 gene in the iaa9-3 and iaa9-5 mutants compared to normal condition by 0,45 fold and 1,78 fold, respectively.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Ekspresi Gen SlDREBA4, Heat Stress, IAA9, Solanum lycopersicum, Thermo-morphogenesis, SlDREBA4 Gene Expression
Subjects:	Q Science > QH Biology > QH301 Biology Q Science > QK Botany T Technology > TP Chemical technology > TP248.27.P55 Plant biotechnology.
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Cory Rafsyanyani
Date Deposited:	18 Jan 2024 04:22
Last Modified:	18 Jan 2024 04:22
URI:	http://repository.its.ac.id/id/eprint/103104

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