Wahid, Nabiilah Aliyyah (2023) Analisis Perbandingan Kemampuan Antioksidan Air Hitam Taman Nasional Sebangau Kalimantan Tengah dengan Antioksidan Sintetis BHT (Butil Hidroksitoluen) Menggunakan Metode DPPH. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Penelitian ini bertujuan untuk membandingkan kemampuan antioksidan air hitam yang didapatkan dari Taman Nasional Sebangau Kalimantan Tengah dengan antioksidan sintetis BHT (Butil Hidroksitoluen). Metode DPPH (2,2-difenil-1-pikrihidrazil) digunakan untuk meneliti kemampuan aktivitas antioksidan dengan mengukur absorbansi maksimum pada DPPH yang didapatkan pada panjang gelombang 517 nm. Sampel air hitam didapatkan dengan mengambil air dari permukaan sungai dan dimasukkan ke dalam botol minum. Sampel yang telah didapatkan didekantasi agar bersih dari kotoran. Sedangkan untuk BHT dibuat larutan dengan konsentrasi 4 ppm dengan mengambil beberapa mL dari larutan induk BHT 100 ppm. Metode DPPH dilakukan dengan cara mencampurkan DPPH dengan air hitam atau BHT kemudian di inkubasi dalam ruangan tertutup selama 30 menit. Setelah 30 menit di ukur absorbansi dari masing-masing sampel dan di plot menggunakan Microsoft excel untuk didapatkan IC50. Hasil dari penelitian ini menunjukkan bahwa perbandingan air hitam:DPPH dengan BHT:DPPH secara berurutan adalah 5,418:2 dan 7,361:2. Dari hasil tersebut dapat disimpulkan bahwa air hitam memiliki kemampuan antioksidan lebih besar dibandingkan dengan antioksidan sintetis BHT. Hal ini didukung oleh hasil skrining fitokimia yang menunjukkan adanya kandungan metabolit sekunder berupa alkaloid dan tannin dalam air hitam
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This study aims to compare the antioxidant ability of black water obtained from Sebangau National Park, Central Kalimantan with synthetic antioxidant BHT (Butyl Hydroxytoluene). The DPPH (2,2-diphenyl-1-picrylhydrazyl) method was applied to examine the antioxidant activity ability by measuring the maximum absorbance of DPPH obtained at a wavelength of 517 nm. Black water samples were obtained by taking water from the surface of the river and put into a drinking bottle. Samples that have been obtained are decanted to be clean from impurities. As for BHT, a solution with a concentration of 4 ppm was made by taking a few mL of 100 ppm BHT mother solution. DPPH method is done by mixing DPPH with black water or BHT then incubated in a closed room for 30 minutes. After 30 minutes, the absorbance of each sample was measured and plotted using Microsoft excel to obtain IC50. The results of this study show that the ratio of black water: DPPH with BHT : DPPH is 5.418 : 2 and 7.361 : 2, respectively. From these results it can be concluded that black water has greater antioxidant ability compared to the synthetic antioxidant BHT. This is supported by the results of phytochemical screening which shows the presence of secondary metabolites in the form of alkaloids and tannins in black water
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Air Hitam, BHT, DPPH, IC50, Skrining Fitokimia; Black Water, BHT, DPPH, IC50, Phytochemical Screening. |
| Subjects: | Q Science > QD Chemistry > QD117.S64 Spectrophotometry Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry Q Science > QD Chemistry > QD75.2 Chemistry, Analytic |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis |
| Depositing User: | Nabiilah Aliyyah Wahid |
| Date Deposited: | 03 Oct 2023 06:56 |
| Last Modified: | 03 Oct 2023 06:56 |
| URI: | http://repository.its.ac.id/id/eprint/103966 |
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