Akbar, Sarazen Shalahuddin (2023) Optimasi Biodekolorisasi Metil Oranye Oleh Bakteri Pseudomonas aeruginosa yang Terimobilisasi pada Matriks SA/PVA/UiO-66 dengan Response Surface Methodology. Other thesis, Institut Teknologi Sepuluh Nopember.
![[thumbnail of ACCESS CLOSED BY AUTHOR]](http://repository.its.ac.id/style/images/fileicons/text.png) |
Text (ACCESS CLOSED BY AUTHOR)
01211940000066-Undergraduate_Thesis.pdf - Accepted Version Restricted to Repository staff only Download (4MB) | Request a copy |
Abstract
Metil oranye (MO) merupakan salah satu jenis pewarna azo yang sering diaplikasikan dalam industri tekstil. Limbah MO diketahui bersifat toksik sehingga berbahaya baik terhadap kesehatan maupun lingkungan. Pada penelitian ini, optimasi biodekolorisasi MO oleh bakteri Pseudomonas aeruginosa (PA) yang terimobilisasi pada matriks SA/PVA/UiO-66 dengan response surface methodology (RSM) telah diteliti. Beads SA/PVA/UiO-66/PA difabrikasi dengan formulasi perbandingan 2; 4; 0,4; 10 (b/v). Beads hasil fabrikasi digunakan untuk uji biodekolorisasi MO dengan optimasi RSM. Model yang digunakan adalah quadratic, dengan variabel-variabel bebas meliputi pH (X1), temperatur (X2), dan waktu inkubasi (X3) serta variabel terikat yaitu persentase biodekolorisasi (Y1). Persamaan regresi linear yang diperoleh yaitu Y = 85,58 - (9,92) A + (1,20) B + (7,57) C - (2,69) A2 - (6,06) B2 - (21,22) C2 - (2,94) AB - (1,29) AC + (1,70) BC. Optimasi yang dipilih dalam penelitian ini menghasilkan persentase biodekolorisasi sebesar 92,497%, dengan pH sebesar 4,693, suhu sebesar 7,023 °C, serta waktu inkubasi selama 7,023 hari. Validasi terhadap optimasi tersebut menghasilkan persentase biodekolorisasi sebesar 93,983%. Telah dilakukan pula karakterisasi dengan menggunakan FTIR dan SEM. Hasil uji reusabilitas terhadap beads SA/PVA/UiO-66/PA sebanyak tiga kali siklus menunjukkan persentase biodekolorisasi masing-masing sebesar 93,983%; 75,94%; dan 5,348%. Aplikasi bakteri PA yang diimobilisasikan pada ada matriks SA/PVA/UiO-66 terbukti dapat melakukan biodekolorisasi MO secara efektif sehingga berpotensi sebagai agen biodegradasi dalam mengatasi pencemaran lingkungan.
======================================================================================================================================
Methyl orange (MO) is a type of azo dye that is often applied in the textile industry. MO waste is known to be toxic, so it is harmful to both health and the environment. In this research, the optimation of MO biodecolorization by Pseudomonas aeruginosa (PA) immobilized on the SA/PVA/UiO-66 matrix was investigated using the response surface methodology (RSM). SA/PVA/UiO-66/PA beads were fabricated with a comparison of 2; 4; 0.4; 10 (b/v). The fabricated beads were used for the MO biodecolorization test with RSM optimization. The model used was quadratic, with independent variables including pH (X1), temperature (X2), and incubation time (X3), as well as the dependent variable was the percentage of biodecolorization (Y1). The linear regression equation obtained was Y = 85,58 - (9.92) A + (1.20) B + (7.57) C - (2.69) A2 - (6.06) B2 - (21, 22) C2 - (2.94) AB - (1.29) AC + (1.70) BC. The optimization chosen in this study resulted in a biodecolorization percentage of 92.497%, with a pH of 4.693, a temperature of 7.023 °C, and an incubation time of 7.023 days. The validation of the optimization resulted in a biodecolorization percentage of 93.983%. Characterizations of beads were carried out using FTIR and SEM. The reusability test results for three cycles of SA/PVA/UiO-66/PA beads showed that the percentage of biodecolorization was 93.983%; 75.94%; and 5.348%. The application of PA bacteria immobilized on the matrix SA/PVA/UiO-66 was proven to be able to biodecolorize MO effectively so that it has the potential as a biodegradation agent in dealing with environmental pollution.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Biodekolorisasi, Metil Oranye, Imobilisasi, Pseudomonas aeruginosa, Response Surface Methodology, Biodecolorization, Methyl Orange, Immobilization, Pseudomonas aeruginosa, Response Surface Methodology |
| Subjects: | Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis |
| Depositing User: | Sarazen Shalahuddin Akbar |
| Date Deposited: | 25 Jan 2024 07:39 |
| Last Modified: | 14 Oct 2024 07:50 |
| URI: | http://repository.its.ac.id/id/eprint/104060 |
Actions (login required)
 |
View Item |