Fatimah, Sabila (2023) Bakteri Multiresisten Logam Berat sebagai Agen Bioremediasi Lahan Kritis sekaligus Agen Hayati yang Berpotensi sebagai Pupuk NPK. Masters thesis, Institut Teknologi Sepuluh Nopember.
![[thumbnail of 6005211007-Master_Thesis.pdf]](http://repository.its.ac.id/style/images/fileicons/text.png) |
Text
6005211007-Master_Thesis.pdf - Accepted Version Restricted to Repository staff only until 1 October 2025. Download (2MB) | Request a copy |
Abstract
Lahan kritis merupakan lahan yang telah mengalami kerusakan sehingga lahan tersebut tidak dapat berfungsi secara baik sesuai dengan peruntukannya. Pencemaran logam berat merupakan salah satu penyebab terjadinya lahan kritis. Alternatif mereduksi logam berat sekaligus memberikan kesuburan terhadap tanah untuk mengatasi lahan kritis dengan memanfaatkan bakteri yang multiresisten logam berat sekaligus sebagai penyedia unsur hara makro nitrogen, fosfat, dan kalium (NPK). Tujuan penelitian adalah mendapatkan bakteri yang multiresisten logam berat sebagai bioakumulator sekaligus penyedia NPK. Bakteri diisolasi dari kawasan mangrove Wonorejo yang sudah mulai tercemar logam berat. Uji resistensi dilakukan terhadap logam Fe, Pb, Cd, dan Hg, pada konsentrasi 1 ppm dengan media NA-logam berat sampai 10 ppm, sedangkan untuk uji bioakumulasi menggunakan media Mineral Salt Medium – Nutrient Broth yang mengandung logam berat 10 ppm. Pengukuran konsentrasi logam berat menggunakan metode spektrofotometri. Identifikasi spesies bioakumulator logam berat dilakukan menggunakan marka gen 16S rRNA. Skrining untuk potensi sebagai pupuk hayati menggunakan uji siderofor dengan media simple double-layered Chrome Azurol Sulphonate Agar (SD-CASA), uji kualitatif penyedia NPK menggunakan metode indeks zona degradasi dan konsentrasinya menggunakan metode spektrofotometri. Hasil uji multiresisten terhadap logam Fe, Pb, Cd, dan Hg konsentrasi 10 ppm, didapatkan enam (6) isolat yaitu WN1, WN4, WN8, WN9, WN10, dan WN12. Uji siderofor didapatkan empat (4) isolat yaitu WN1, WN4, WN9, dan WN12. Tiga (3) isolat dengan zona siderofor tertinggi teridentifikasi sebagai spesies Bacillus cereus (WN4), Pseudomonas migulae (WN9) dan Bacillus cereus (WN12). Isolat WN4 dan WN12 pada pohon filogenetik diketahui tergolong ke dalam Bacillus cereus sensu-lato. Isolat WN4, WN9, dan WN12 memiliki potensi sebagai bioakumulator logam berat Hg dengan efisiensi bioakumulasi 99,90%. Isolat WN4 dan WN9 menunjukkan potensi penyedia NPK secara kualitatif dan dapat menyediakan nitrat sebesar 21,15% (WN4), dan 31,05% (WN9). Isolat WN12 tidak dapat menyediakan nitrat.
=================================================================================================================================
Critical land is land that has been damaged so that the land cannot function properly according to its designation. Heavy metal pollution is one of the causes of critical land. An alternative to reducing heavy metals as well as providing soil fertility to overcome critical land by utilizing bacteria that are multiresistant to heavy metals as well as providing the macro nutrients nitrogen, phosphate and potassium (NPK). The aim of the research was to find bacteria that are multiresistant to heavy metals as a bioaccumulator as well as a provider of NPK. The bacteria were isolated from the Wonorejo mangrove area which had begun to be polluted by heavy metals. Resistance tests were carried out on the metals Fe, Pb, Cd, and Hg, at a concentration of 1 ppm with NA-heavy metal media up to 10 ppm, while for the bioaccumulation test using Mineral Salt Medium – Nutrient Broth media containing 10 ppm heavy metals. Measurement of heavy metal concentrations using the spectrophotometric method. Identification of heavy metal bioaccumulator species was carried out using 16S rRNA gene markers. Screening for potential as a biological fertilizer used a siderophore test with simple double-layered Chrome Azurol Sulphonate Agar (SD-CASA) media, a qualitative test for NPK providers using the degradation zone index method and its concentration using a spectrophotometric method.
The results of the multiresistant test for Fe, Pb, Cd, and Hg at a concentration of 10 ppm, obtained six (6) isolates namely WN1, WN4, WN8, WN9, WN10, and WN12. The siderophore test obtained four (4) isolates namely WN1, WN4, WN9, and WN12. Three (3) isolates with the highest siderophore zones were identified as Bacillus cereus (WN4), Pseudomonas migulae (WN9) and Bacillus cereus (WN12) species. Isolates WN4 and WN12 on the phylogenetic tree are known to belong to Bacillus cereus sensu-lato. Isolates WN4, WN9, and WN12 have potential as bioaccumulators of heavy metal Hg with a bioaccumulation efficiency of 99.90%. Isolates WN4 and WN9 showed potential as NPK providers qualitatively and could provide nitrates of 21.15% (WN4) and 31.05% (WN9). Isolate WN12 cannot provide nitrate.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Bioremediasi, Lahan kritis, Logam berat, NPK, 16S rRNA, Bioremediation, Critical land, Heavy metals |
| Subjects: | Q Science > QH Biology Q Science > QR Microbiology Q Science > QR Microbiology > QR74.8 Bacteria S Agriculture > S Agriculture (General) > S654.5 Biofertilizers |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46101-(S2) Master Thesis |
| Depositing User: | Sabila Fatimah |
| Date Deposited: | 01 Sep 2023 06:13 |
| Last Modified: | 01 Sep 2023 06:13 |
| URI: | http://repository.its.ac.id/id/eprint/104915 |
Actions (login required)
 |
View Item |