Pengembangan Elektroda Ag (Silver) Film Modifikasi Molecular Imprinting Polymer (MIP) untuk Deteksi Dini Kanker Darah

Zulfa, Vinda Zakiyatuz (2023) Pengembangan Elektroda Ag (Silver) Film Modifikasi Molecular Imprinting Polymer (MIP) untuk Deteksi Dini Kanker Darah. Doctoral thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Kanker darah adalah penyakit yang ditandai dengan pertumbuhan tidak normal dari sel darah putih (leukosit). Seperti kebanyakan kanker lain, gejala timbul saat mencapai stadium lanjut. Penanganan kanker pada stadium lanjut memperkecil kesembuhan dan berakhir pada kematian pasien. Deteksi dini kanker sangat dibutuhkan untuk mempercepat proses kesembuhan pasien. Para peneliti telah mengembangkan deteksi dini kanker yang lebih murah dan cepat salah satunya dengan menggunakan biomarker kanker albumin. Metode elektrokimia digunakan karena lebih sederhana, cepat dan sensitif. Sensitifitas sensor juga didapatkan dengan penambahan reseptor biologis. Molecular Imprinting Polymer (MIP) digunakan sebagai pengganti reseptor biologis karena persiapan yang mudah dan stabilitas tinggi. Pada penelitian ini, biosensor elektrokimia dicetak secara molekuler untuk mendeteksi Bovine Serum Albumin (BSA) berdasarkan film Ag modifikasi MIP. Elektroda Indhium Thin Oxide (ITO) dimodifikasi dengan film Ag dan Molecular Imprinting Polymer (MIP). MIP disintesis melalui elektropolimerisasi dengan BSA sebagai template, chitosan (CS) sebagai monomer fungsional, dan glutaraldehyde (GA) sebagai agen pengikat silang. Karakterisasi elektroda modifikasi MIP dilakukan dengan Scanning Electron Microscope (SEM), X-Ray Diffraction Analysis (XRD), Fourier-Transform Infrared Spectroscopy (FT-IR), Cyclic Voltammetry (CV), dan Electric Impedance Spectroscopy (EIS). Pengujian secara kuantitatif didapatkan dengan EIS dan Differential Pulse Voltammetry (DPV). Dengan karakterisasi dan pengujian ini, diperoleh nilai sensitifitas 57.55 µA mM-1cm-1 dan 47,125 Ohm mM-1 cm-1. Pada pengujian yang dilakukan dengan serum buffer (PBS). Nilai batas deteksi (LOD) adalah 7 × 10-19 g/dl-1 pada rentang konsentrasi 10-19 hingga 10-1 g/dl. Nilai ini lebih rendah dibandingkan penelitian yang telah ada sebelumnya. Pengaruh molekul lain juga diamati dan menunjukkan bahwa sensor memiliki sensitifitas yang tinggi, reproduktifitas dan stabilitas yang baik, sehingga dapat diregenerasi.
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Blood cancer is a disease characterized by abnormal growth of white blood cells (leukocytes). Like most other cancers, symptoms develop when they reach an advanced stage. Treatment of cancer at an advanced stage minimizes recovery and ends in the death of the patient. Early detection of cancer is needed to speed up the patient's recovery process. Researchers have developed a cheaper and faster early detection of cancer, one of which is by using the cancer biomarker albumin. In this study, an electrochemical biosensor was printed molecularly to detect Bovine Serum Albumin (BSA) based on MIP modified Ag films. Indhium Thin Oxide (ITO) electrodes were modified with Ag films and Molecular Imprinting Polymer (MIP). MIP was synthesized by electropolymerization with BSA as a template, chitosan (CS) as a functional monomer, and glutaraldehyde (GA) as a crosslinking agent. The characterization of the modified MIP electrode was carried out by Scanning Electron Microscope (SEM), X-Ray Diffraction Analysis (XRD), Fourier-Transform Infrared Spectroscopy (FT-IR), Cyclic Voltammetry (CV), and Electric Impedance Spectroscopy (EIS). Quantitative testing was carried out with EIS and Differential Pulse Voltammetry (DPV). With this characterization and testing, sensitivity values of 57.55 µA mM-1cm-1 and 47.125 Ohm mM-1cm-1 were obtained on CV and EIS measurements. In tests carried out with serum buffer (PBS). The limit of detection value (LOD) was 7 × 10-19 g/dl-1 in the concentration range from 10-19 to 10-1 g/dl. This value is lower than previous research. The influence of other molecules was also observed and showed that the sensor has high sensitivity, good reproducibility and stability, so it can be regenerated.

Item Type:	Thesis (Doctoral)
Uncontrolled Keywords:	Ag, BSA, Elektrokimia, Kanker Darah, MIP, Electrochemical, Blood Cancer, MIP.
Subjects:	Q Science > QD Chemistry > QD115 Electrochemical analysis Q Science > QD Chemistry > QD553 Electrochemistry. Electrolysis R Medicine > R Medicine (General) > R856.2 Medical instruments and apparatus. R Medicine > R Medicine (General) > R857.M3 Biomedical materials. Biomedical materials--Testing.
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Physics > 45001-(S3) PhD Thesis
Depositing User:	Vinda Zakiyatuz Zulfa
Date Deposited:	08 Sep 2023 04:15
Last Modified:	08 Sep 2023 04:15
URI:	http://repository.its.ac.id/id/eprint/104936

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