Azotobacter sebagai bioakumulatior merkuri

Khotimah, Kusnul (2014) Azotobacter sebagai bioakumulatior merkuri. Other thesis, Insititut Teknologi Sepuluh Nopember.

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Abstract

Merkuri merupakan logam berat paling toksik dibandingkan dengan logam berat lainnya. Beberapa bakteri ada yang resisten merkuri. Salah satu genus bakteri resisten merkuri dan mampu mengakumulasi merkuri yaitu Azotobacter. Azotobacter merupakan bakteri pemfiksasi nitrogen bebas non simbiotik yang melimpah di daerah rhizosfer lahan pertanian dan merupakan bakteri penghasil EPS yang dapat berfungsi sebagai pengkhelat logam. Penelitian ini bertujuan untuk mendapatkan isolat Azotobacter yang resisten terhadap merkuri HgCl2, dan mengukur kemampuan bioakumulasinya terhadap HgCl2. Isolasi bakteri Azotobacter dilakukan dengan media selektif Azotobacter. uji resistensi HgCl2 dilakukan dengan streak agar miring dan kemampuan bioakumulasi diukur dengan metode serapan atom serta uji viabilitas menggunakan metode pour plate. Analisis beda nyata dengan ANOVA pada taraf 5% dilanjutkan dengan uji beda nyata terkecil (BNT). Tiga isolat Azotobacter dari lahan eco urban farming ITS resisten terhadap HgCl2 sampai 20 mg/L yaitu A5, A6, dan A9. Efisiensi bioakumulasi yang tertinggi pada pemaparan HgCl2 5 mg/L yaitu isolat A5 (89%) dan A9 (87%).
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Mercury is the most toxic heavy metals compared with other heavy metals. Some bacteria are resistant to mercury. One of mercury resistant bacteria genus and able to accumulate of mercury is Azotobacter. Azotobacter is free-living nitrogen fixing bacteria which abundant in rhizosfer area of agricultural land and it is EPS producing bacteria that can serve as chelating metals. This research aims to get Azotobacter isolates resistant to mercury HgCl2, and measure the ability of mercury biaccumulation. Isolation of Azotobacter by selective Azotobacter media, resistance test by streak at slant agar, and bioaccumulation ability measured by atomic absorption method and vaibility test using pour plate method. Observed data analyzed by ANOVA, continued by Least Significant Difference (LSD) test, Both test at level 5 %. Three Azotobacter isolates from eco urban farming ITS land are resistant until 20 mg/L of HgCl2 which is A5, A6 and A9. The highest efficiency bioaccumlation on exposure 5 mg/L HgCl2 is A5 (89%) and A9 (87%)

Item Type:	Thesis (Other)
Additional Information:	RSBi 571.954 663 Kho a-1, 2014
Uncontrolled Keywords:	Azotobacter, bioakumulator, merkuri, uji resistensi, bioaccumulator, mercury, resistance test.
Subjects:	Q Science > QR Microbiology > QR74.8 Bacteria
Divisions:	Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Mr. Marsudiyana -
Date Deposited:	10 Jan 2024 04:30
Last Modified:	10 Jan 2024 04:30
URI:	http://repository.its.ac.id/id/eprint/105436

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