Potensi Isolat Bakteri Bacillus Dan Pseudomonas Dalam Mendegradasi Plastik Dengan Metode Kolom Winogradsky

Fadlilah, Fiki Rahmah (2014) Potensi Isolat Bakteri Bacillus Dan Pseudomonas Dalam Mendegradasi Plastik Dengan Metode Kolom Winogradsky. Other thesis, Insititut Teknologi Sepuluh Nopember.

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Abstract

Karakter plastik yang sulit terdegradasi di lingkungan mendorong penelitian ini untuk mengetahui potensi bakteri Bacillus dan Pseudomonas koleksi Laboratorium Mikrobiologi Biologi ITS dalam mendegradasi beberapa jenis plastik uji. Metode yang digunakan adalah Kolom Winogradsky dengan botol ukuran 1,5 l, berisi pasir steril : medium Salt = 1:1. Plastik uji yang digunakan adalah kantong plastik merah, biru, hitam, putih dan hijau ukuran 15 x 4 cm. Botol diisi 15 potongan plastik. Proses biodegradasi dilakukan selama 4 bulan, dengan parameter berat kering plastik, dan pengukuran densitas bakteri uji pada λ 600 nm. Isolat bakteri Bacillus dan Pseudomonas memiliki potensi dalam mendegradasi plastik uji, karena mampu tumbuh dalam kolom Winogradsky selama 4 bulan masa inkubasi. Isolat Bacillus memiliki Optical Density pada plastik hitam : putih : biru : hijau : merah sebesar 0,210: 0,234: 0,180: 0,183: 0,172. Isolat Pseudomonas pada plastik hitam : putih : biru : hijau : merah sebesar 0,196: 0,200: 0,160: 0,161: 0,176. Pada isolat Bacillus persen degradabilitas plastik warna hijau, putih, hitam, biru, merah secara berturut adalah 1,9%, 2,3%, 1,6%, 1,6%, 1,6%. Pada isolat Pseudomonas secara berturut adalah 1,7%, 2,2%, 1,4%, 1,6%, 1,7%.
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The characters of plastic that hard to be degraded in the environment to encourage this study to determine the potential of the bacteria Bacillus and Pseudomonas (ITS Biology Microbiology Laboratory collection) in some types of plastics test. The method used is the Winogradsky column with a 1.5 l bottle size, containing sterile sand: medium Salt = 1:1. Plastic test used was a plastic bag of red, blue, black, white and green size 15 x 4 cm. Bottles filled with 15 pieces of plastic. Biodegradation process carried out for 4 months, with a plastic dry weight parameters, and measurements of bacterial density test at λ 600 nm. Isolates of Bacillus and Pseudomonas bacteria have the potential to degrade the plastic test, because it is able to grow in the Winogradsky column for 4-month incubation period. Optical Density of Bacillus isolates on the black plastic: white: blue: green: red by 0.21: 0.234: 0.180: 0.183: 0.172. Pseudomonas isolates on black plastic: white: blue: green: red by 0.196: 0.200: 0.160: 0.161: 0.176. In Bacillus isolates percent degradability of green plastic, white, black, blue, red, respectively were 1.9%, 2.3%, 1.6%, 1.6%, 1.6%. In Pseudomonas isolates, respectively were 1.7%, 2.2%, 1.4%, 1.6%, 1.7%.

Item Type:	Thesis (Other)
Additional Information:	RSBi 620.192 042 23 Fad p-1, 2014
Uncontrolled Keywords:	Kantong Plastik., Biodegradasi, Kolom Winogradsky, Bacillus, Pseudomonas, Plastic Bags, Biodegradation, Winogradsky column,
Subjects:	Q Science > QR Microbiology > QR74.8 Bacteria
Divisions:	Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Mr. Marsudiyana -
Date Deposited:	10 Jan 2024 06:47
Last Modified:	10 Jan 2024 06:47
URI:	http://repository.its.ac.id/id/eprint/105438

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