Pengaruh Metode Ekstraksi yang Berbeda pada Ciplukan (Physalis angulata) Terhadap Penghambatan Aktivitas α-Amilase Secara In-Vitro

Mokodompis, Gilbert Billstein (2024) Pengaruh Metode Ekstraksi yang Berbeda pada Ciplukan (Physalis angulata) Terhadap Penghambatan Aktivitas α-Amilase Secara In-Vitro. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Physalis angulata (ciplukan) merupakan tanaman yang mengandung beberapa senyawa bioaktif seperti withangulatin A, flavonoid, alkaloid, dan terpenoid. Pendekatan terapeutik yang terkenal untuk pencegahan dan pengelolaan diabetes adalah dengan menghambat kinerja enzim α-amilase. Hasil studi sebelumnya menggunakan molecular docking menunjukan bahwa senyawa bioaktif P.angulata dapat menghambat enzim α-amilase sehingga dapat dikatakan secara farmakologis berpotensi sebagai antidiabetes. Senyawa bioaktif dalam tumbuhan dapat diperoleh dengan metode ekstraksi antara lain maserasi dan refluks. Kadar dan jenis senyawa bioaktif yang terekstraksi dapat dipengaruhi oleh jenis pelarut, rasio pelarut, dan sensitifitas terhadap suhu (thermolabile). Berdasarkan hal tersebut sangat penting dilakukan pemilihan metode ekstraksi “senyawa bioaktif” P. angulata yang optimal dan potensinya sebagai antidiabetes secara in-vitro. Penelitian ini bertujuan untuk mengevaluasi pengaruh perbedaan metode ekstraksi pada P. angulata terhadap kemampuan penghambatan α-amilase secara in vitro. Dari nilai persentase inhibisi ke-4 sub-fraksi yaitu GM1= 95,53%, GM2= 94,00%, GR1= 94,51%, dan GR2= 93,91% dapat menghambat 0,4 U/mL sebesar ekuivalen dengan 0,375- 0,382 U/mL. Hasil uji ANOVA one-way diperoleh signifikansi (sig) 0.214 dimana p > 0.05.
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Physalis angulata (ciplukan) is a plant that contains several bioactive compounds such as withangulatin A, flavonoids, alkaloids, and terpenoids. A well-known therapeutic approach for the prevention and management of diabetes is to inhibit the activity of the α-amylase enzyme. Previous studies using molecular docking have shown that the bioactive compounds in P. angulata can inhibit the α-amylase enzyme, suggesting pharmacological potential as an antidiabetic agent. Bioactive compounds in plants can be obtained through extraction methods such as maceration and reflux. The quantity and types of bioactive compounds extracted can be influenced by the type of solvent, solvent ratio, and sensitivity to temperature (thermolabile). Therefore, it is crucial to choose the optimal extraction method for the "bioactive compounds" in P. angulata and evaluate its potential as an in-vitro antidiabetic agent. This study aims to evaluate the impact of different extraction methods on P. angulata regarding its ability to inhibit α-amylase in vitro. The inhibition percentages for the four sub-fractions, namely GM1 = 95.53%, GM2 = 94.00%, GR1 = 94.51%, and GR2 = 93.91%, were able to inhibit 0.4 U/mL equivalent to 0.375-0.382 U/mL. The one-way ANOVA test resulted in a significance (sig) of 0.214, where p > 0.05.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	α-Amilase, Ciplukan, Maserasi, Refluks, Uji in vitro, In vitro, Maceration, Reflux
Subjects:	Q Science > QH Biology > QH301 Biology
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Gilbert Billstein Mokodompis
Date Deposited:	26 Jan 2024 07:05
Last Modified:	26 Jan 2024 07:05
URI:	http://repository.its.ac.id/id/eprint/105664

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