Bakteri Selulolitik, Lipolitik, dan Proteolitik dari Bioaktivator untuk Pakan Ikan

Wicaksono, Alfauzi Satrio (2024) Bakteri Selulolitik, Lipolitik, dan Proteolitik dari Bioaktivator untuk Pakan Ikan. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Indonesia mengalami peningkatan konsumsi ikan dari tahun 2011 hingga 2021 berdasarkan data Kementerian Kelautan dan Perikanan. Adanya peningkatan konsumsi ikan tersebut menyebabkan terjadinya meningkatnya kebutuhan akan kualitas nutrisi pakan ikan. Pakan dengan nutrisi yang berkualitas berpotensi dihasilkan dari limbah organik yang telah difermentasi oleh bakteri, salah satunya melalui penambahan bakteri bioaktivator. Bioaktivator dibuat dari air cucian beras, tempe, serta limbah buah dan sayuran sebagai sumber inokulum bakteri selulolitik, lipolitik, dan proteolitik. Penelitian ini menggunakan 3 jenis bioaktivator sebagai inokulum bakteri. Tujuan dari penelitian ini adalah mengetahui kemampuan bakteri selulolitik, proteolitik, dan lipolitik isolat bakteri berdasarkan indeks zona bening serta mengetahui keanekaragaman bakteri pada ketiga bioaktivator berdasarkan karakter biokimia menurut Bergey’s Manual of Determinative Bacteriology 9th Edition. Metode identifikasi dalam penelitian ini, meliputi: identifikasi kemampuan selulolitik, lipolitik, dan proteolitik berdasarkan indeks zona bening pada media spesifik; serta identifikasi karakter biokimia bakteri sampai tingkat genus. Indeks selulolitik paling optimal setiap bioaktivator sebesar 2,21 cm (isolat B34), 2,07 cm (isolat M24), dan 2,11 cm (isolat E51). Berdasarkan indeks lipolitik isolat bakteri paling tinggi di setiap bioaktivator adalah isolat B48 sebesar 5,82 cm, isolat M81 sebesar 2,43 cm, dan isolat E52 sebesar 0,61 cm. Pada indeks proteolitik terbesar setiap bioaktivator dimiliki oleh isolat B75 sebesar 1,52 cm, isolat M24 sebesar 1,60 cm, dan isolat E44 sebesar 13,57 cm. Genus bakteri yang teridentifikasi dari bioaktivator 1 meliputi, Bacillus sp., Vibrio sp., dan Brucella sp. Pada bioaktivator 2, teridentifikasi genus Lactobacillus sp., Bacillus sp., dan Lactobacillus sp. Genus bakteri yang teridentifikasi pada bioaktivator 3 adalah Lactobacillus sp., Pseudomonas sp., dan Brucella sp.
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Indonesia experienced an increase in fish consumption from 2011 to 2021 based on data from the Ministry of Marine Affairs and Fisheries. The increase in fish consumption has led to an increase in the need for quality fish feed nutrition. Feed with quality nutrition can potentially be produced from organic waste that has been fermented by bacteria, one of which is through the addition of bioactivator bacteria. Bioactivators are made from rice washing water, tempeh, and fruit as well as vegetable waste as the source of inoculum for cellulolytic, lipolytic, and proteolytic bacteria. This study used 3 types of bioactivators as bacterial inoculum. The purpose of this study was to determine the ability of cellulolytic, lipolytic, and proteolytic bacterial isolates based on the clear zone index and to determine the diversity of bacteria in the three bioactivators based on biochemical characters according to Bergey's Manual of Determinative Bacteriology 9th Edition. Identification methods in this study include: identification of cellulolytic, lipolytic, and proteolytic abilities based on the clear zone index on specific media; and identification of bacterial biochemical characters until the genus level. The most optimal cellulolytic index of each bioactivator was 2,21 cm (isolate B34), 2,07 cm (isolate M24), and 2,11 cm (isolate E51). Based on the lipolytic index, the highest bacterial isolates in each bioactivator were isolate B48 at 5,82 cm, isolate M81 at 2,43 cm, and isolate E52 at 0,61 cm. The largest proteolytic index in each bioactivator was owned by isolate B75 by 1,52 cm, isolate M24 by 1,60 cm, and isolate E44 by 13,57 cm. The bacterial genus identified from bioactivator 1 includes, Bacillus sp., Vibrio sp., and Brucella sp. In bioactivator 2, the genus Lactobacillus sp., Bacillus sp., and Lactobacillus sp. The bacterial genus identified in bioactivator 3 is Lactobacillus sp., Pseudomonas sp., and Brucella sp.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Bakteri, Bioaktivator, Lipolitik, Proteolitik, Selulolitik, Bacteria, Bioactivator, Cellulolytic, Lipolytic, Proteolytic
Subjects:	Q Science Q Science > QH Biology Q Science > QR Microbiology Q Science > QR Microbiology > QR74.8 Bacteria
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Alfauzi Satrio Wicaksono
Date Deposited:	05 Feb 2024 06:12
Last Modified:	05 Feb 2024 06:12
URI:	http://repository.its.ac.id/id/eprint/106054

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