Pengaruh Asam Tartarat Terhadap Aktivitas Antibakteri Nanopartikel Perak

Anggraeni, Zalina Nilam (2024) Pengaruh Asam Tartarat Terhadap Aktivitas Antibakteri Nanopartikel Perak. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Beberapa tahun terakhir, material berukuran nano menjadi topik popular dan menarik atensi para ilmuwan dikarenakan abilitas dari strukturnya yang lebih unggul jika dibandingkan dengan bulk particles serta dapat diaplikasikan ke berbagai bidang termasuk biomedis. Nanopartikel perak (AgNPs) banyak digagas karena ukuran dan bentuknya yang dapat diaplikasikan sebagai antioksidan, bahan biosensor, produk kosmetik, komponen elektronik, dan antibakteri, sehingga menjadikannya titik fokus penelitian. Sintesis AgNPs menggunakan metode reduksi kimia dengan perak nitrat sebagai prekursor, asam askorbat sebagai agen pereduksi dengan variasi capping agent yaitu PVP dan kombinasi PVP-asam tartarat telah berhasil dilakukan dengan response surface methodology secara khusus desain CCF. Hasil plot 3D dapat menunjukkan titik optimum dari rentang eksperimental yang dilakukan yaitu konsentrasi asam tartarat di bawah 0,5%, waktu sonikasi berkisar antara 16 hingga 29 menit, dan suhu dalam rentang 47,5°C hingga 67,5°C. Nanopartikel perak yang disintesis menunjukkan kisaran panjang gelombang maksimum sebesar 420 nm, berbentuk bulat, bermuatan negatif dan memiliki ukuran sekitar 20,23 nm untuk AgNPs/PVP dan 17,66 nm untuk AgNPs/PVP-tartarat dengan nilai PDI lebih dari 0,05 serta menunjukkan bahwa penambahan asam tartarat akan berpengaruh signifikan pada pembentukan AgNPs pada konsentrasi di bawah 0,5%, namun berhasil mempersempit distribusi ukuran dan menurunkan nilai zeta potensial AgNPs serta sukses sebagai antibakteri karena lebih baik untuk menghambat bakteri B. subtilis dan E. coli dengan zona hambat berturut-turut sebesar 7,76 mm dan 6,31 mm daripada AgNPs tanpa asam tartarat yang menghambat bakteri B. subtilis dan E. coli dengan zona hambat berturut-turut sebesar 3,0 mm dan 4,5 mm.
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In recent years, nano-sized materials have become a popular and intriguing topic, take the attention of scientists due to their superior structural abilities compared to bulk particles and their applicability in various fields, including biomedicine. Silver nanoparticles (AgNPs) have gained significant attention due to their size and shape, making them suitable for applications such as antioxidants, biosensor materials, cosmetic products, electronic components, and antibacterial agents, making them a focal point of research. The synthesis of AgNPs using the chemical reduction method with silver nitrate as a precursor and ascorbic acid as a reducing agent, along with variations in capping agents such as PVP and a combination of PVP and tartaric acid, has been successfully carried out using response surface methodology, specifically the CCF design. The results of 3D plots demonstrate the optimum points within the experimental ranges, including tartaric acid concentrations below 0.5%, sonication times ranging from 16 to 29 minutes, and temperatures within the range of 47.5°C to 67.5°C. The AgNPs synthesized exhibit a maximum wavelength around 420 nm, a spherical shape, a negative charge, and has a size approximately 20,23 nm for AgNPs/PVP and 17,66 nm for AgNPs/PVP-tartaric with a PDI value greater than 0.05. The addition of tartaric acid will significantly affect the formation of AgNPs at concentration below 0,5% but it successfully narrowing the size distribution and reduces the zeta potential values of AgNPs. Moreover, these AgNPs demonstrate effective antibacterial properties, as they exhibit superior inhibition of B. subtilis and E. coli compared to AgNPs without tartaric acid. The respective inhibition zones are 7,76 nm and 6,31 nm, whereas AgNPs without tartaric acid show inhibition zones of 3,0 nm and 4,5 nm against B. subtilis and E. coli

Item Type:	Thesis (Other)
Uncontrolled Keywords:	nanopartikel perak, asam tartarat, antibakterial, response surface methodology, silver nanoparticles, tartaric acid, antibacterial
Subjects:	T Technology > TP Chemical technology > TP248 Nanogels. Nanoparticles.
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Anggraeni Zalina Nilam
Date Deposited:	15 Feb 2024 02:37
Last Modified:	15 Feb 2024 02:37
URI:	http://repository.its.ac.id/id/eprint/107048

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