Risbandini, Christin (2024) Studi Perbandingan Anatomi Dan Profil Protein Tanaman Porang (Amorphophallus Muelleri Blume) Dalam Satu Siklus Hidup. Masters thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Tanaman Porang (Amorphophallus muelleri Blume) merupakan tanaman berumbi dengan nilai ekonomi tinggi serta potensi untuk dikembangkan sebagai alternatif pengganti sumber pangan utama. Dalam satu siklus hidup tanaman porang, terjadi perubahan struktur anatomi organ vegetatif dan profil protein pada umbi. Bagian jaringan penyusun organ daun, stomata mengalami penurunan sebesar 23-30%. Jaringan epidermis, penurunan terjadi sebesar 9-16%, dan jaringan parenkim, sebesar 25-95%. Pada jaringan berkas pembuluh mengalami penurunan sebesar 9-11%, sedangkan jaringan mesofil sebesar 100%. Bagian jaringan penyusun organ batang, penurunan pada jaringan epidermis sebesar 9-15%. Pada jaringan korteks sebesar 24-56%, dan pada jaringan berkas pembuluh sebesar 100%. Bagian jaringan penyusun organ akar, jaringan epidermis mengalami penurunan sebesar 17-18%., jaringan korteks sebesar 14-16%, dan jaringan berkas pembuluh sebesar 81-86%. Kerapatan total kristal kalsium oksalat pada umbi porang saat dormansi mengalami kenaikan sebesar 13-18%, sedangkan kerapatan butir amilum mengalami kenaikan sebesar 100% pada saat sebelum dormansi. Hasil pengamatan anatomi menunjukkan adaptasi pada tanaman sebagai respons terhadap perubahan kondisi lingkungan yang tidak menguntungkan, yaitu cekaman kekeringan. Profil protein dengan SDS-PAGE didapatkan pita protein dengan berat molekul sekitar 26,72 - 56,28 kDa. Hal tersebut ditunjukkan dengan perbedaan jumlah pita protein, berat molekul pita protein, dan ketebalan pita protein yang dihasilkan
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The Porang plant (Amorphophallus muelleri Blume) is a bulbous plant with high economic value and the potential to be developed as an alternative to the main food source. In one life cycle of the porang plant, changes occur in the anatomical structure of the vegetative organs and the protein profile of the tubers. The tissue part that makes up the leaf organs, stomata, decreased by 23-30%. Epidermal tissue, decreased by 9-16%, and parenchymal tissue, by 25-95%. The vascular bundle tissue decreased by 9-11%, while the mesophyll tissue decreased by 100%. As part of the tissue that makes up the stem organs, the decrease in epidermal tissue is 9-15%. In the cortex tissue it is 24-56%, and in the vascular bundle tissue it is 100%. The tissue parts that make up the root organ, epidermal tissue decreased by 17-18%, cortex tissue by 14-16%, and vascular bundle tissue by 81-86%. The total density of calcium oxalate crystals in porang tubers during dormancy increased by 13-18%, while the density of starch grains increased by 100% before dormancy. The results of anatomical observations show adaptation in plants as a response to changes in unfavorable environmental conditions, namely drought stress. The protein profile using SDS-PAGE showed a protein band with a molecular weight of around 26.72 - 56.28 kDa. This is indicated by differences in the number of protein bands, the molecular weight of the protein bands, and the thickness of the protein bands produced
Item Type: | Thesis (Masters) |
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Uncontrolled Keywords: | Dormansi, profil protein, struktur anatomi, SDS PAGE; Dormancy, protein profile, anatomical structure, SDS PAGE |
Subjects: | Q Science Q Science > QH Biology Q Science > QK Botany Q Science > QK Botany > QK710 Plant physiology Q Science > QP Physiology Q Science > QP Physiology > QP519.9.E434 Electrophoresis. Q Science > QP Physiology > QP624 Molecular biology. |
Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46101-(S2) Master Thesis |
Depositing User: | Christin Risbandini |
Date Deposited: | 29 Feb 2024 06:34 |
Last Modified: | 29 Feb 2024 06:34 |
URI: | http://repository.its.ac.id/id/eprint/107190 |
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