Pemanfaatan Substrat Urin Sapi dalam Fermentasi Urease dan Aplikasinya pada Biostabilisasi Tanah Dasar Jalan

Haqq, Annida (2024) Pemanfaatan Substrat Urin Sapi dalam Fermentasi Urease dan Aplikasinya pada Biostabilisasi Tanah Dasar Jalan. Masters thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Teknologi biosementasi merupakan teknologi inovatif terbaru untuk meningkatkan kualitas bahan-bahan geologi seperti struktur material tanah yang tidak stabil dan berkekuatan rendah. Teknologi biosementasi dapat dilakukan melalui dua metode: a) Microbially-Induced Carbonate Precipitation (MICP); dan b) Enzyme-Induced Carbonate Precipitation (EICP). Namun, dalam metode MICP sulit untuk mengontrol pertumbuhan mikroba yang digunakan, sebab dibutuhkan kondisi lingkungan optimum untuk pertumbuhannya. Oleh karena itu, pada penelitian ini dilakukan eksplorasi metode EICP yang memanfaatkan enzim urease untuk mengendapkan kalsium karbonat, sehingga dapat menjadi biostabilizer bagi material tanah. Penelitian ini bertujuan mengetahui kondisi optimum pada proses produksi urease dengan faktor pH, suhu (oC), dan konsentrasi urin sapi (%). Selain itu juga mengevaluasi pengaruh penambahan larutan EICP pada material tanah terhadap kekuatan tanah, direpresentasikan oleh nilai daya dukung tanah (DDT). Optimasi faktor produksi urease dilakukan dengan metode RSM (Response Surface Method). Karakterisasi urease meliputi uji aktivitas (metode Nessler), analisa kadar protein (Bradford), uji Titik Isoelektrik, dan pemurnian parsial dengan Amonium Sulfat. Pengukuran DDT menggunakan uji CBR (California Bearing Ratio) dan visualisasi presipitasi kalsium karbonat menggunakan uji SEM (Scanning Electron Microscopy). Aktivitas urease tertinggi hasil optimasi RSM diperoleh pada kondisi pH 7, suhu 37,5oC, dan konsentrasi urin 5% yaitu sebesar 3.052,46 U/mL dengan kadar protein 0,0135 mg/mL. Setelah dipurifikasi (45-60% Amonium Sulfat), aktivitas spesifik urease meningkat menjadi 354.364,8 U/mg. Nilai CBR pada material tanah yang ditambah EICP (urease) yaitu sebesar 41,46% dan tanah tanpa penambahan EICP sebesar 30,48%, menunjukkan peningkatan nilai CBR sebesar 1,36 kali lipat. Visualisasi SEM menampilkan hasil reaksi urease berupa pembentukan kalsium karbonat, terutama kalsit, yang mampu mengikat antar partikel tanah sehingga dapat meningkatkan kekuatan dan kepadatan tanah
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Biocementation technology is a new innovative technology to improve the quality of geological materials such as unstable and low strength soil material structures. The biocementation technique can be performed through two methods: a) Microbially-Induced Carbonate Precipitation (MICP); and b) Enzyme-Induced Carbonate Precipitation (EICP). However, in the MICP method, it is difficult to control the optimum environmental conditions for microbial growth. Therefore, this research explores a bio-inspired technique using the EICP method that utilises urease to precipitate calcium carbonate so that it can be a biostabiliser for soil materials. This research aims to determine the optimum conditions in the urease production process with the factors of pH, temperature (oC), and cow urine concentration (%), and evaluate the effect of adding EICP solution to soil material on the value of soil bearing capacity. Optimisation of urease production factors was carried out using RSM (Response Surface Method). Characterisation of urease included activity test (Nessler method), protein content analysis (Bradford), Isoelectric Point test, and enzyme purification with Ammonium Sulfate. Soil bearing capacity measurement used CBR (California Bearing Ratio) test, while visualisation of calcium carbonate precipitation used SEM (Scanning Electron Microscopy) test. The highest activity of the optimisation results was obtained under conditions of pH 7, temperature 37.5oC, and 5% urine concentration of 3,052.46 U/mL with a protein content of 0.0135 mg/mL. After purification (45-60% Ammonium Sulfate), the specific activity increased to 354,364.8 U/mg. The CBR value of soil samples added with EICP (urease) was 41.46%, while the CBR value of soil without EICP addition was 30.48%. SEM visualisation shows the results of the urease reaction, namely the formation of calcium carbonate, especially calcite, which is able to bind between soil particles so as to increase soil strength and density

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	EICP, biostabilisasi, tanah dasar, urease, urin; EICP, biostabilizer, subgrade, urease, urine
Subjects:	Q Science > QR Microbiology > QR74.8 Bacteria
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46101-(S2) Master Thesis
Depositing User:	Annida Haqq
Date Deposited:	15 Feb 2024 02:17
Last Modified:	15 Feb 2024 02:17
URI:	http://repository.its.ac.id/id/eprint/107399

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