Sukma, Chindy Melati (2024) Aktivitas Antioksidan dan Penghambatan Tirosinase Senyawa Trisindolina 1 dalam Menghambat Hiperpigmentasi Akibat Radiasi Ultraviolet pada Kulit Mencit (Mus musculus). Masters thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Radiasi UV menyebabkan terjadinya hiperpigmentasi. Hiperpigmentasi dapat diatasi dengan agen anti-hiperpigmentasi yang bekerja dengan cara menghambat aktivitas enzim tirosinase. Selain itu, pemberian antioksidan juga dapat mencegah hiperpigmentasi. Trisindolina memiliki potensi sebagai agen anti-hiperpigmentasi karena dapat menghambat aktivitas enzim tirosinase dan bekerja sebagai antioksidan. Penelitian ini bertujuan untuk mempelajari aktivitas antioksidan dan penghambatan tirosinase dari trisindolina 1 untuk menghambat hiperpigmentasi akibat radiasi UV. Uji aktivitas antioksidan dan penghambatan tirosinase dilakukan untuk memperoleh nilai IC50 trisindolina 1. Selanjutnya, dilakukan uji in vivo untuk mengetahui efek biologis pemberian trisindolina 1 pada kulit dorsal mencit. Vitamin C digunakan sebagai kontrol. Analisis histomorfologi dilakukan untuk mengetahui kandungan spesies oksigen reaktif (ROS), kadar melanin, dan kadar tirosinase. Hasilnya didapatkan bahwa IC50 trisindolina 1 sebagai antioksidan yaitu sebesar 419,9 μg/mL dan tergolong sebagai antioksidan sangat lemah. Namun, IC50 trisindolina 1 sebagai inhibitor tirosinase yaitu sebesar 87,55 μg/mL dan termasuk dalam inhibitor tirosinase kuat. Analisis histomorfologi menghasilkan adanya penurunan kandungan ROS, kadar melanin, dan kadar tirosinase masing-masing sebesar 3,633%, 20,463%, dan 27,006%. Hasil ini mengasumsikan bahwa trisindolina 1 berpotensi sebagai agen anti-hiperpigmentasi
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UV radiation causes hyperpigmentation. Hyperpigmentation can be treated with anti-hyperpigmentation agents which work by inhibiting the activity of the tyrosinase enzyme. In addition, providing antioxidants can be used to prevent hyperpigmentation. Trisindoline has potential as an anti-hyperpigmentation agent because it inhibits the activity of the tyrosinase and works as an antioxidant. This study analyzed the antioxidant activity and tyrosinase inhibition of trisindoline 1 to inhibit UV-induced hyperpigmentation. Antioxidant activity and tyrosinase inhibition assays were carried out to obtain the IC50 value of trisindoline 1. Subsequent tests were carried out in vivo to determine the biological effect of trisindoline 1 on the dorsal skin of mice treated with UV radiation. Vitamin C was used as control because it is a commercial substance as hyperpigmentation therapy agent. Histomorphological analysis was carried out to determine the content of reactive oxygen species (ROS), melanin levels, and tyrosinase levels. The result showed that IC50 of trisindoline 1 as antioxidant was 419,9 μg/mL which was classified as a very weak antioxidant. However, the IC50 of trisindoline 1 as a tyrosinase inhibitor is 87,55 μg/mL which is included as a strong tyrosinase inhibitor. Histomorphological analysis resulted in decrease in ROS content, melanin levels, and tyrosinase levels by 3,633%, 20,463%, and 27,006% respectively. These results assume that trisindoline 1 has potential as an anti-hyperpigmentation agent.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Antioksidan, Hiperpigmentasi, Melanogenesis, Radiasi UV, Tirosinase, Trisindolina 1 |
| Subjects: | Q Science Q Science > QH Biology > QH301 Biology R Medicine > RL Dermatology |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46101-(S2) Master Thesis |
| Depositing User: | Sukma Chindy Melati |
| Date Deposited: | 19 Feb 2024 07:27 |
| Last Modified: | 19 Feb 2024 07:27 |
| URI: | http://repository.its.ac.id/id/eprint/107434 |
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