Produksi Lakase oleh Trametes versicolor dengan Sistem Submerged Fermentation dan Aplikasinya untuk Biodegradasi Phenanthrene

Fitriya, Salsabila Aida (2024) Produksi Lakase oleh Trametes versicolor dengan Sistem Submerged Fermentation dan Aplikasinya untuk Biodegradasi Phenanthrene. Masters thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Pengeboran minyak mentah selalu menimbulkan dampak negatif bagi lingkungan sekitar akibat cemaran fraksi minyak Polycyclic Aromatic Hydrocarbon (PAH). Phenanthrene (PHE) adalah salah satu dari 16 senyawa PAH yang diidentifikasi oleh Badan Perlindungan Lingkungan AS sebagai polutan prioritas berdasarkan kelimpahan dan toksisitasnya. Biodegradasi secara enzimatik dapat memberikan hasil yang lebih ekonomis, efektif, dan efisien. Lakase dari White Rot Fungi (WRF) yakni Trametes versicolor memiliki potensi redoks tertinggi dan terbukti dapat mengoksidasi PHE di bawah kondisi aerobik statis. Produksi dengan Submerged Fermentation (SmF) diketahui merupakan salah satu strategi untuk meningkatkan hasil produksi lakase dalam waktu yang relatif singkat. Produksi lakase dan proses degradasinya dipengaruhi oleh beberapa faktor sehingga optimasi produksi menggunakan Response Surface Methodology (RSM) menjadi solusi. RSM digunakan untuk menentukan kondisi operasional sistem yang optimal atau untuk menentukan wilayah yang memenuhi spesifikasi operasional. Faktor yang akan digunakan yakni pH, konsentrasi substrat (%), dan konsentrasi mediator (mM) menggunakan sistem SmF untuk produksi lakase. Lakase yang telah diproduksi secara optimum kemudian akan dilakukan karakterisasi berupa titik isoelektrik, kadar protein, dan pemurnian parsial. Kemudian untuk mengetahui kondisi optimum degradasi PHE oleh lakase akan dilakukan dengan faktor berupa pH, temperatur (°C), dan konsentrasi mediator (mM). PHE yang telah diberi perlakuan lakase kemudian akan dilakukan uji viabilitas mikroba dan uji fitotoksisitas. Hasil dari penelitian yang telah dilakukan yaitu didapatkan kondisi optimum produksi lakase oleh isolat T. versicolor yakni pH 5, konsentrasi substrat 5%, dan konsentrasi CuSO4 0,5 mM. Nilai aktivitas crude lakase tertinggi yakni 7129,9 U/ml. Hasil efisiensi degradasi PHE tertinggi yakni sebesar 97,3% dengan kondisi optimum degradasi yakni pH 5, suhu 30°C, dan konsentrasi mediator 1,5 mM.
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Drilling for crude oil always has a negative impact on the surrounding environment due to contamination of Polycyclic Aromatic Hydrocarbons (PAH) oil fractions. Phenanthrene (PHE) is one of 16 PAH compounds identified by the US Environmental Protection Agency as priority pollutants based on their abundance and toxicity. Enzymatic biodegradation can provide more economical, effective and efficient results. Laccase from White Rot Fungi (WRF), namely Trametes versicolor, has the highest redox potential and has been proven to oxidize PHE under static aerobic conditions. Production using Submerged Fermentation (SmF) is known to be a strategy to increase laccase production results in a relatively short time. Laccase production and its degradation process are influenced by several factors so that production optimization using Response Surface Methodology (RSM) is a solution. RSM is used to determine optimal system operational conditions or to determine areas that meet operational specifications. The factors that will be used are pH, substrate concentration (%), and mediator concentration (mM) using a SmF system for laccase production. Laccase that has been produced optimally will then be characterized in the form of isoelectric point, protein content and partial purification. Then, to determine the optimum conditions for PHE degradation by laccase, factors such as pH, temperature (°C) and mediator concentration (mM) will be carried out. PHE that has been treated with laccase will then be subjected to a microbial viability test and phytotoxicity test. The results of this research were that the optimum conditions for laccase production by T. versicolor isolates were obtained, namely pH 5, substrate concentration 5%, and CuSO4 concentration 0,5 mM. The highest crude laccase activity value was 7129.92 U/ml. The highest PHE degradation efficiency result was 97.302% with optimum degradation conditions, namely pH 5, temperature 30°C, and mediator concentration of 1,5 mM.

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	Lakase, Phenanthrene, Submerge fermentation, Trametes versicolor Response Surface Methodology, Laccase
Subjects:	Q Science > QH Biology Q Science > QH Biology > QH301 Biology Q Science > QH Biology > QH75 Nature conservation. Landscape protection. Biodiversity conservation. Endangered species and ecosystems (General). Habitat conservation. Ecosystem management. Conservation biology Q Science > QR Microbiology
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46101-(S2) Master Thesis
Depositing User:	Fitriya Salsabila Aida
Date Deposited:	15 Feb 2024 05:49
Last Modified:	15 Feb 2024 05:49
URI:	http://repository.its.ac.id/id/eprint/107436

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