Pengembangan Kertas Nanoselulosa Semi Transparan untuk Deteksi Kolorimetri Multipleks

Adila, Farah Nita (2024) Pengembangan Kertas Nanoselulosa Semi Transparan untuk Deteksi Kolorimetri Multipleks. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Microfluidic paper-based analytical device (μPAD) adalah perangkat analitik berbasis kertas untuk mendeteksi senyawa kimia atau bio tertentu. Kertas μPAD biasanya memanfatkan substrat selulosa alami salah satunya berasal dari bambu. Bambu merupakan tumbuhan dengan masa tumbuh yang cepat, ketersediaan melimpah di Indonesia dan mengandung selulosa yang tinggi sekitar 40-50%. Pada penelitian ini, bambu yang digunakan adalah bambu hitam dengan ukuran 30 mesh dan 60 mesh. Serat selulosa diekstraksi dengan dua metode yaitu metode mekanik dan metode hidrolisis asam. Selulosa yang telah diekstraksi akan diubah menjadi skala nano pada metode mekanik melalui perlakuan ultrasonikasi sedangkan metode hidrolisis asam melalui perlakuan asam kuat. Kertas nanoselulosa dicetak pada cawan petri berukuran 6 cm dengan konsentrasi 2,5%, 5% dan 10%. Karakteristik kertas metode mekanik yaitu rata-rata sudut kontak 50,17°, rata-rata kristalinitas 54,05%, rata-rata porositas 24,54%, rata-rata konsentrasi gugus hidroksil 0,82% dan transmitansi dalam rentang 4-30%. Karakteristik kertas metode hidrolisis asam yaitu rata-rata sudut kontak 51,36°, rata-rata kristalinitas 49,71%, rata-rata porositas 15,75%, rata-rata konsentrasi gugus hidroksil 0,92% dan transmitansi dalam rentang 7-16%. Berdasarkan hasil pengujian flow rate menunjukkan kertas 30 mesh 2,5% metode mekanik dan kertas 60 mesh 2,5% metode hidrolisis asam memiliki kelajuan aliran fluida tertinggi. Berdasarkan hasil pengujian kolorimetri menunjukkan adanya peningkatan skala intensitas RGB dan grayscale seiring penambahan konsentrasi dopamin dan NADH. Uji kolorimetri dopamin memiliki sensitivitas tertinggi pada kertas 30 mesh 2,5% mekanik sebesar 4,26 mM-1 dan linieritas tertinggi pada kertas 60 mesh 2,5% hidrolisis asam sebesar 0,88. Uji kolorimetri NADH memiliki sensitivitas tertinggi pada kertas 60 mesh 2,5% hidrolisis asam sebesar 4,81 mM-1 dan linieritas tertinggi pada kertas 30 mesh 2,5% sebesar 0,54.
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A microfluidic paper-based analytical device (μPAD) is a paper-based analytical device used to detect specific chemical or biological compounds. μPADs typically utilize natural cellulose substrates, one of which is derived from bamboo. Bamboo is a fast-growing plant, abundantly available in Indonesia, and contains high cellulose content, around 40-50%. In this study, the bamboo used is black bamboo with sizes of 30 mesh and 60 mesh. Cellulose fibers are extracted using two methods, namely the mechanical method and the acid hydrolysis method. The extracted cellulose is converted to the nanoscale using ultrasonic treatment in the mechanical method, while strong acid treatment is used in the acid hydrolysis method. Nanocellulose paper is cast onto 6 cm Petri dishes with concentrations of 2.5%, 5%, and 10%. The mechanical method paper have an average contact angle of 50.17°, an average crystallinity of 54.05%, an average porosity of 24.54%, an average hydroxyl group concentration of 0.82%, and a transmittance value in the range of 4-30%. The acid hydrolysis method paper have an average contact angle of 51.36°, an average crystallinity of 49.71%, an average porosity of 15.75%, an average hydroxyl group concentration of 0.92%, and a transmittance value in the range of 7-16%. Based on the flow rate test results, the 30 mesh 2.5% mechanical method paper and the 60 mesh 2.5% acid hydrolysis method paper have the highest fluid flow rates. Based on the results of the colorimetric tests, there is an increase in the scale of RGB and grayscale intensity with the addition of dopamine and NADH concentrations. The dopamine colorimetric test had the highest sensitivity on 30 mesh 2.5% mechanical method of 4.26 mM-1 and the highest linearity on 60 mesh 2.5% acid hydrolysis method of 0.88. The NADH colorimetric test had the highest sensitivity on 60 mesh 2.5% acid hydrolysis method of 4.81 mM-1 and the highest linearity on 30 mesh 2.5% paper of 0.54.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	μPAD, Hidrolisis Asam, Mekanik, Nanoselulosa, Semi Transparan, Acid Hydrolysis, Mechanical, Nanocellulose, Semi-Transparent
Subjects:	T Technology > T Technology (General) > TA404 Materials--Biodegradation T Technology > TP Chemical technology > TP1140 Polymers T Technology > TP Chemical technology > TP248 Nanogels. Nanoparticles.
Divisions:	Faculty of Industrial Technology and Systems Engineering (INDSYS) > Physics Engineering > 30201-(S1) Undergraduate Thesis
Depositing User:	Farah Nita Adila
Date Deposited:	05 Jul 2024 05:12
Last Modified:	05 Jul 2024 05:12
URI:	http://repository.its.ac.id/id/eprint/108155

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