Fabrikasi dan Karakterisasi Nanopartikel Perak dari Ekstrak Daun Mimba (Azadirachta Indica) sebagai Antibakteri Bacillus Subtilis dengan Bantuan Cahaya Biru

Nadiyah, Diyana Nasihatin (2024) Fabrikasi dan Karakterisasi Nanopartikel Perak dari Ekstrak Daun Mimba (Azadirachta Indica) sebagai Antibakteri Bacillus Subtilis dengan Bantuan Cahaya Biru. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Nanopartikel perak (AgNPs) telah banyak dimanfaatkan karena sifat antibakteri dan aplikasinya yang luas sehingga menarik minat dan perhatian peneliti terhadap sintesis AgNPs yang ramah lingkungan. Pada penelitian ini, AgNPs disintesis hijau menggunakan ekstrak daun Azadirachta indica. Diameter partikel rata-rata AgNPs dihitung dengan mikroskop elektron (SEM). Analisis SEM menunjukkan bahwa AgNPs yang terbentuk berbentuk bola dan terdistribusi dalam kisaran 52–116 nm. Sifat kristal AgNPs memiliki struktur face-centered cubic yang dikonfirmasi oleh puncak pola difraksi sinar-X yang sesuai bidang (111), (200), (220), (311), dan (222). Pembentukan AgNPs juga dikonfirmasi dari analisis pektrofotometer UV-Vis yang menunjukkan panjang gelombang maksimum di 460 nm. Aktivitas antibakteri AgNPs diteliti terhadap bakteri Bacillus subtilis. Radiasi light-emitting Diode (LED) diketahui dapat menyebabkan fotoinaktivasi sel bakteri karena proses fotosensitisasi. Oleh karena itu, pengujian antibakteri juga dilakukan dengan penyinaran LED biru dengan panjang gelombang 460–465 nm. Hasilnya menunjukkan bahwa AgNPs yang diiradiasi LED biru memiliki efek antibakteri yang lebih signifikan daripada AgNPs saja.
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Silver nanoparticles (AgNPs) have been widely utilized due to their antibacterial properties and wide applications, thus attracting the interest and attention of researchers towards the synthesis of environmentally friendly AgNPs. In this study, AgNPs were synthesized using Azadirachta indica leaf extract. The average particle diameter of AgNPs was calculated by electron microscopy (SEM). SEM analysis showed that the AgNPs formed were spherical and distributed in the range of 52–116 nm. The crystalline nature of AgNPs has a face-centered cubic structure, which is confirmed by the X-ray diffraction pattern peaks corresponding to the (111), (200), (220), (311), and (222) planes. The formation of AgNPs was also confirmed by UV-Vis spectrophotometer analysis, which showed a maximum wavelength of 460 nm. The antibacterial activity of AgNPs was investigated against Bacillus subtilis bacteria. Light Emitting Diode (LED) radiation is known to cause photoinactivation of bacterial cells due to the photosensitization process. Therefore, antibacterial testing was also conducted with blue LED irradiation with a wavelength of 460–465 nm. The results showed that blue LED-irradiated AgNPs had a more significant antibacterial effect than AgNPs alone.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Nanopartikel perak, Azadirachta indica, Efek Antibakteri, LED, Fotosensitisasi, Silver Nanoparticles, Antibacterial Effect, Photosensitization
Subjects:	Q Science > QC Physics > QC173.4.C63 Composite materials
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Physics > 45201-(S1) Undergraduate Thesis
Depositing User:	Diyana Nasihatin Nadiyah
Date Deposited:	26 Jul 2024 00:52
Last Modified:	26 Jul 2024 00:52
URI:	http://repository.its.ac.id/id/eprint/108965

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