Pengaruh Phenylethylamine (PEA) Pada Proses Penyembuhan Luka Kulit Menggunakan Model Mencit (Mus musculus L.) Berdasarkan Pengamatan Histologis

Khairunnisa, Aqiilah (2024) Pengaruh Phenylethylamine (PEA) Pada Proses Penyembuhan Luka Kulit Menggunakan Model Mencit (Mus musculus L.) Berdasarkan Pengamatan Histologis. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Luka pada tubuh yang normal secara biologis mampu mengembalikan integritasnya melalui proses penyembuhan luka. Phenylethylamine (PEA) merupakan senyawa memiliki potensi dalam mempercepat penyembuhan luka. Uji sebelumnya secara in vivo masih terbatas pada data luas penutupan luka dan belum ada data proses dinamis fase-fase penyembuhan luka. Oleh karena itu, dilakukan penelitian lanjutan yang bertujuan untuk mengetahui pengaruh variasi konsentrasi PEA terhadap penyembuhan luka pada mencit (Mus musculus L.) berdasarkan gambaran histologis. Sampel kulit dilukai dengan skin biopsy punch dan setiap hari diberi PEA dengan konsentrasi 0 μg/mL (D0); 12,5 μg/mL (D1); 25 μg/mL (D2); 50 μg/mL(D3) selama 14 hari dengan kontrol (K) menggunakan kulit yang tidak dilukai. Sampel kulit dipreparasi menggunakan metode paraffin dan pewarnaan Hematoksilin-eosin dengan gambaran histologis yang dianalisis secara kualitatif (struktur jaringan), kuantitatif (ketebalan epidermis), dan semi-kuantitatif (scoring karakteristik peyembuhan luka pada struktur epidermis, serat kolagen, dan folikel rambut). Data dianalisis secara statistik menggunakan ANOVA one-way dan Tukey. Hasil menunjukkan bahwa luka kulit dengan penambahan PEA (D1, D2, dan D3) memiliki tingkat kesembuhan luka yang lebih tinggi secara signifikan dibandingkan dengan kulit tanpa penambahan PEA (D0), dan hampir sama atau menyerupai kulit kontrol (normal). Variasi konsentrasi PEA tidak berbeda signifikan yang mengindikasikan pengaruh yang sama dalam percepatan penyembuhan luka.
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Biologically normal wounds on the body are able to restore their integrity through the wound healing process. Phenylethylamine (PEA) is a compound that has the potential to accelerate wound healing. Previous in vivo tests were still limited to data on the extent of wound closure and there was no data on the dynamic process of wound healing phases. Therefore, further research was carried out which aimed to determine the effect of variations in PEA concentration on wound healing in mice (Mus musculus L.) based on histological images. Skin samples were injured with a skin biopsy punch and given PEA every day at a concentration of 0 μg/mL (D0); 12,5 μg/mL (D1); 25 μg/mL (D2); 50 μg/mL (D3) for 14 days with control (K) using uninjured skin. Skin samples were prepared using the paraffin method and Hematoxylin-eosin staining with histological images analyzed qualitatively (tissue structure), quantitatively (epidermal thickness), and semi-quantitatively (scoring wound healing characteristics of the epidermal structure, collagen fibers, and hair follicles). Data were analyzed statistically using one way ANOVA and Tukey. The results showed that skin wounds with the addition of PEA (D1, D2, and D3) had a significantly higher wound healing rate compared to skin without the addition of PEA (D0), and were almost the same or similar to control (normal) skin. Variations in PEA concentration did not differ significantly, indicating the same effect in accelerating wound healing.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Hematoksilin-eosin, Penyembuhan Luka, Phenylethylamine, Hematoxylin-eosin, Wound Healing
Subjects:	Q Science > QH Biology Q Science > QH Biology > QH301 Biology
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Aqiilah khairunnisa
Date Deposited:	04 Sep 2024 02:00
Last Modified:	04 Sep 2024 02:00
URI:	http://repository.its.ac.id/id/eprint/109622

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