Pengaruh Nanopartikel Perak Hasil Sintesis Hijau Ekstrak Kulit Jeruk Nipis (Citrus aurantifolia) Pada Kultur In Vitro Tomat Kultivar Micro-Tom iaa9-5

Alfatikhatunnisa, Maulida (2024) Pengaruh Nanopartikel Perak Hasil Sintesis Hijau Ekstrak Kulit Jeruk Nipis (Citrus aurantifolia) Pada Kultur In Vitro Tomat Kultivar Micro-Tom iaa9-5. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Buah tomat mengandung beragam fitokimia seperti alkaloid solanin, asam malat, asam sitrat, adenine, dan beberapa jenis vitamin. Berdasarkan kandungan yang ada di dalam tomat tersebut, permintaan konsumen akan produk tomat di Indonesia mengalami peningkatan yang cukup signifikan. Salah satu upaya untuk meningkatkan produktivitas tanaman tomat adalah melalui perakitan varietas tanaman tomat baru yang tahan terhadap kekeringan dan suhu tinggi seperti tomat kultivar Micro-Tom iaa9-5. Micro-Tom mutan iaa9-5 adalah hasil dari mutasi gen IAA9 yang memiliki karakter tahan terhadap suhu tinggi dan menghasilkan buah partenokarpi dan berat buah yang cenderung konsisten. Gen IAA9 dapat membuka peluang mempelajari lebih dalam hubungan respon tanaman terhadap nanopartikel perak (AgNP). AgNP merupakan suatu logam perak dalam ukuran nano yang sering digunakan dalam upaya perbanyakan tanaman melalui kultur jaringan. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh beberapa konsentrasi AgNP terhadap pertumbuhan tomat kultivar MicroTom iaa9-5 secara in vitro. Penelitian ini menggunakan eksplan nodus tanaman tomat cv. Micro-Tom iaa9-5 yang tersedia di Laboratorium Biosains dan Teknologi Tumbuhan. Media yang digunakan dalam penelitian ini yaitu media MS dengan penambahan sintesis hijau nanopartikel perak ekstrak kulit jeruk nipis (Citrus aurantifolia) dengan konsentrasi 0 µg/L, 12,5 µg/L, 30 µg/L dan 75 µg/L. Adapun pengamatan yang diamati adalah rerata jumlah nodus, daun, dan berat basah tanaman serta menghitung persentase kalogenesis dan organogenesis eksplan. Semua data dianalisis dengan kualitatif menggunakan metode ANOVA one way dan post-hoc Tukey test pada taraf kepercayaan 95% untuk melihat perbedaan signifikan antar perlakuan. Hasil pada penelitian ini menunjukkan bahwa persentase organogenesis 100% dan kalogenesis 0%. Selain itu, nanopartikel perak hasil sintesis hijau ekstrak kulit jeruk nipis (Citrus aurantifolia) berpengaruh terhadap beberapa parameter pertumbuhan pada kultur in vitro tomat Micro-Tom iaa9-5 seperti tinggi tanaman, berat basah, jumlah daun dan jumlah nodus.
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Tomatoes contain various phytochemicals such as solanine alkaloids, malic acid, citric acid, adenine, and several types of vitamins. Based on the content contained in tomatoes, consumer demand for tomato products in Indonesia has increased quite significantly. One effort to increase the productivity of tomato plants is through developing new tomato plant varieties that are resistant to drought and high temperatures, such as the Micro-Tom iaa9-5 tomato cultivar. Micro-Tom iaa9-5 mutant is the result of healing the IAA9 gene which has the character of being resistant to high temperatures and produces parthenocarpic fruit and fruit weight that tends to be consistent. The IAA9 gene could open up opportunities to learn more about the relationship between plant responses to silver nanoparticles (AgNP). AgNP is a silver metal in nano size which is often used in plant propagation efforts through tissue culture. The aim of this research was to determine the effect of several AgNP concentrations on the growth of the tomato cultivar Micro-Tom iaa9-5 in vitro. This research used tomato plant node explants cv. Micro-Tom iaa9-5 available at the Plant Bioscience and Technology Laboratory. The media used in this research is MS media with the addition of green synthetic silver nanoparticles from lime peel (Citrus aurantifolia) extract with concentrations of 0 µg/L, 12.5 µg/L, 30 µg/L and 75 µg/L. The observations were the average number of nodules, leaves and wet weight of the plants as well as calculating the percentage of callogenesis and organogenesis of the explants. All data were analyzed qualitatively using the one way ANOVA method and Tukey's post-hoc test at a confidence level of 95% to see significant differences between treatments. The results of this study show that the percentage of organogenesis is 100% and callogenesis is 0%. In addition, silver nanoparticles resulting from the green synthesis of lime peel extract (Citrus aurantifolia) have an effect on several growth parameters in in vitro cultures of Micro-Tom iaa9-5 tomatoes such as plant height, wet weight, number of leaves and number of nodules.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	AgNP, Citrus aurantifolia, IAA9, Kultur in Vitro, Micro-tom iaa9-5 AgNP, Citrus aurantifolia, IAA9, In Vitro Culture, Micro-tom iaa9-5
Subjects:	Q Science > QH Biology > QH301 Biology Q Science > QK Botany
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Maulida Alfatikhatunnisa
Date Deposited:	08 Aug 2024 08:40
Last Modified:	08 Aug 2024 08:40
URI:	http://repository.its.ac.id/id/eprint/111927

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