Rahmah, Salsabila (2024) Pengaruh Panjang Gelombang Spesifik Pada Sistem Terapi Fotodinamik Sel Kanker Paru A549 Menggunakan Fotosensitizer Riboflavin Konjugat Green Synthesis Nanopartikel Perak (Rf-AgNP). Other thesis, Institut Teknologi Sepuluh Nopember.
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5001201138-Undergraduate_Thesis.pdf - Accepted Version Restricted to Repository staff only until 1 October 2026. Download (5MB) | Request a copy |
Abstract
Tujuan penelitian ini adalah mengevaluasi pengaruh panjang gelombang spesifik pada uji in vitro terapi fotodinamik (PDT) sel A549 yang menggunakan fotosensitizer Rf-AgNP, dengan AgNP hasil sintesis menggunakan ekstrak kulit jeruk nipis (Citrus aurantifolia). Penyinaran PDT menggunakan 3 variasi panjang gelombang LED, yaitu LED A (370–375 nm), LED B (410–420 nm), dan LED C (440–445 nm) serta 1 variasi tanpa LED. Hasil spektrofotometri UV-Vis, absorbansi maksimum AgNP pada 412–428 nm, sedangkan fotosensitizer Rf-AgNP 367–377 nm dan 439–451 nm. Hasil XRD mengonfirmasi adanya Ag pada sampel AgNP hasil sintesis dengan struktur kubik pusat muka (FCC), tetapi terdapat pengotor. SEM menunjukkan partikel AgNP berukuran 351,4 nm; 204,2 nm; 396,9 nm, dan 350,1 nm dengan bentuk bulat, permukaan kasar, terbentuk agregasi dan aglomerasi serta berpori. EDX menunjukkan komposisi berat dan kandungan atom Ag dalam sampel AgNP sebesar 28,08% dan 4,77%, serta mengonfirmasi adanya elemen lain (pengotor) dalam komposisi sampel AgNP. Dari evaluasi rata-rata persentase sel hidup A549 setelah PDT, dapat dibuktikan pengaruh panjang gelombang pada uji in vitro PDT dengan LED C (440–445 nm) memiliki efek sitotoksik paling signifikan, diikuti oleh non-LED, kemudian LED B (410–420 nm), dan yang paling tidak toksik adalah LED A (370–375 nm).
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The aim of this study was to evaluate the effect of specific wavelengths on the in vitro photodynamic therapy (PDT) of A549 cells using the Rf-AgNP photosensitizer, with AgNPs synthesized using lime peel extract (Citrus aurantifolia). PDT irradiation was carried out using 3 variations of LED wavelength, LED A (370–375 nm), LED B (410–420 nm), and LED C (440–445 nm) as well as 1 variation without LED. UV-Vis spectrophotometry results show that the maximum absorbance of AgNPs is 412–428 nm, while the Rf-AgNP photosensitizer are 367–377 nm and 439–451 nm. The XRD results confirmed the presence of Ag in the synthesized AgNP sample with a face-centered cubic (FCC) structure, but there were impurities. SEM shows AgNP particles measuring 351,4 nm; 204,2nm; 396,9 nm, and 350,1 nm with a round shape, rough surface, aggregation, agglomeration, and porous formation. EDX showed that the weight composition (wt. %) and atomic content (at. %) of Ag in the AgNP sample were 28,08% and 4,77% and confirmed the presence of other elements (impurities) in the composition of the AgNP sample. From the evaluation of the average percentage of viable A549 cells after PDT, it can be proven that the influence of wavelength in the in vitro PDT test with LED C (440–445 nm) has the most significant cytotoxic effect, followed by non-LED, then LED B (410–420 nm), and the least toxic is LED A (370–375 nm).
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