Shanti, Salsabilla Nur (2024) Biodekolorisasi Metilen Biru Oleh Bakteri Uncultured Pseudomonas sp. Clone 034571 018 Yang Terimobilisasi Pada Matriks SA-PVA. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Industri seperti tekstil, kulit, kertas, plastik, kosmetik dan yang lain menggunakan pewarna dalam prosesnya, sehingga menghasilkan sejumlah besar air limbah perwarna. Metilen Biru (MB) merupakan salah satu bahan yang paling banyak digunakan dalam industri pewarna. Konsentrasi tinggi penggunaan MB dapat membahayakan kesehatan manusia jika terhirup atau tertelan dan menimbulkan permasalahan lingkungan. Oleh karena itu diperlukan suatu metode untuk mendegradasi limbah MB. Penanganan yang efisien dalam mengatasi masalah ini, salah satunya dengan pengembangan metode biodegradasi limbah pewarna MB menggunakan bakteri isolat Uncultured Pseudomonas sp. Clone 034571 018 (JN1) dari lahar Gunung Semeru. Tujuan penelitian ini adalah untuk mengetahui kemampuan bakteri JN1 yang terimobilisasi matriks sodium alginat (SA) dan polivinil alkohol (PVA) dalam mendekolorisasi MB. Proses dekolorisasi MB dilakukan menggunakan konsentrasi pewarna 10 mg/L dengan dua metode, yaitu free cell dan terimobilisasi untuk bakteri hidup dan mati dengan waktu inkubasi 10 hari. Hasil penelitian menunjukkan bahwa kemampuan degradasi menggunakan imobilisasi lebih besar dari pada free cell. Persentase dekolorisasi oleh imobilisasi SA-PVA-JN1 kondisi hidup, SA-PVA-JN1 kondisi mati, dan SA-PVA (tanpa bakteri) masing-masing sebesar 95,18%, 62,63%, dan 52,43%. Pada metode free cell JN1 hidup dan JN1 mati didapatkan persentase masing-masing hanya mencapai 51,16% dan 34,20%. Hal ini menunjukkan bahwa SA-PVA-JN1 dalam kondisi hidup memiliki tingkat dekolorisasi tertinggi, yaitu 95,18%, sehingga dapat digunakan sebagai alternatif untuk menghilangkan limbah pewarna MB dalam mengurangi pencemaran dalam lingkungan air.
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Industries such as textiles, leather, paper, plastics, cosmetics and others use dyes in their processes, resulting in a large amount of colored wastewater. Methylene Blue (MB) is one of the most used materials in the dye industry. High concentrations of MB use can harm human health if inhaled or ingested and cause environmental problems. Therefore, a method is needed to degrade MB waste. Efficient handling in overcoming this problem, one of which is by developing a method of biodegradation of MB dye waste using bacteria isolate Uncultured Pseudomonas sp. clone 034571 018 (JN1) from the lava of Mount Semeru. The aim of this research was to determine the ability of JN1 bacterium immobilized with sodium alginate (SA) and polyvinyl alcohol (PVA) matrices to decolorize MB. The MB decolorization process was carried out using a dye concentration of 10 mg/L with two methods, namely free cell and immobilized for live and dead bacteria in a 10-day incubation period. The results of this study showed that the degradation ability using immobilization was greater than the free cell. The percentage of decolorization by SA-PVA-JN1 immobilization of live JN1, SA-PVA-JN1 of dead JN1, and SA-PVA (without JN1) were 95.18%, 62.63%, and 52.43%, respectively. In the free cell method, live JN1 and dead JN1 obtained percentages of only 51.16% and 34.20%, respectively. These shows that SA-PVA-JN1 in live conditions has the highest decolorization level, namely 95.18%, so it can be used as an alternative to remove MB dye waste so as to reduce pollution in the water environment.
Item Type: | Thesis (Other) |
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Uncontrolled Keywords: | Biodegradasi, Dekolorisasi, Free Cell, Imobilisasi, Metilen Biru, SA-PVA, Biodegradation, Decolorization, Free Cell, Immobilization, Methylene Blue, SA-PVA |
Subjects: | Q Science Q Science > Q Science (General) Q Science > QD Chemistry Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry |
Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis |
Depositing User: | Salsabilla Nur Shanti |
Date Deposited: | 07 Aug 2024 03:53 |
Last Modified: | 07 Aug 2024 03:53 |
URI: | http://repository.its.ac.id/id/eprint/112610 |
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