Biodekolorisasi Metil Oranye oleh Uncultured Pseudomonas sp. Clone 034571 018 yang Terimobilisasi pada Matriks SA-PVA

Rachmadani, Novianti Ika (2024) Biodekolorisasi Metil Oranye oleh Uncultured Pseudomonas sp. Clone 034571 018 yang Terimobilisasi pada Matriks SA-PVA. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Pertumbuhan industri tekstil mencapai lebih dari $800 miliar dan lebih dari 10.000 jenis pewarna yang digunakan di seluruh dunia. Salah satu pewarna pada limbah tekstil, yaitu Metil Oranye (MO). MO merupakan golongan pewarna azo yang sulit untuk dihilangkan dan berbahaya bagi manusia maupun organisme air karena toksisitasnya. Oleh karena itu, perlu dilakukan pengurangan pewarna MO yang ada di limbah tekstil di perairan. Tujuan penelitian ini adalah mendekolorisasi MO menggunakan isolat bakteri Uncultured Pseudomonas sp. Clone 034571 018 (JN1) yang diisolasi dari lahar Gunung Semeru yang diimobilisasikan dengan matriks dari campuran sodium alginate (SA) dan polivinil alkohol (PVA) dengan tiga kondisi yaitu bakteri hidup, mati, dan tanpa bakteri. Pada dekolorisasi MO digunakan konsentrasi MO 10 mg/L dengan dua metode yaitu free cell dan imobilisasi dengan waktu inkubasi selama 10 hari. Hasil penelitian ini menunjukkan bahwa kemampuan degradasi menggunakan imobilisasi SA-PVA-JN1 kondisi hidup, SA-PVA-JN1 kondisi mati, dan SA-PVA (tanpa bakteri) memiliki persentase dekolorisasi masing-masing sebesar 87,98%, 40,97%, dan 25,97%. Nilai ini lebih besar dari pada free cell JN1 hidup dan free cell JN1 mati yang nilainya masing-masing sebesar 61,83% dan 17,80%. Hal tersebut menunjukkan bahwa SA-PVA-JN1 dapat dijadikan sebagai alternatif untuk menghilangkan limbah pewarna MO sehingga dapat mengurangi pencemaran pada perairan.
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The textile industry has grown to more than $800 billion and more than 10,000 types of dyes are used worldwide. One of a dye in textile waste is Methyl Orange (MO). MO is a class of azo dyes that are difficult to remove and are harmful to humans and aquatic organisms due to their toxicity. Therefore, it is necessary to reduce MO dyes present in textile waste in waters. The aim of this research was to decolorize MO using bacterial isolate Uncultured Pseudomonas sp. Clone 034571 018 (JN1), isolated from the lava of Mount Semeru, which was immobilized with a matrix of a mixture of sodium alginate (SA) and polyvinyl alcohol (PVA) with three conditions namely live, dead, and without bacteria. In MO decolorization, 10 mg/L MO concentration was used with two methods, namely free cell and immobilization with an incubation time of 10 days. The results showed that the degradation ability using SA-PVA-JN1 immobilization in living condition, SA-PVA-JN1 in dead condition, and SA-PVA (without bacteria) had a decolorization percentage of 87.98%, 40,97%, and 25.97%, respectively. This value is greater than the live JN1 free cell and dead JN1 free cell whose values were 61.83% and 17.80%, respectively. These results indicated that SA-PVA-JN1 can be used as an alternative to remove MO dye waste so as to reduce pollution in waters.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Bakteri, Dekolorisasi, Imobilisasi, Metil Oranye, SA-PVA, Bacteria, Decolorization, Immobilization, Methyl Orange, SA-PVA
Subjects:	Q Science > QD Chemistry > QD117.S64 Spectrophotometry Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry Q Science > QP Physiology > QP517.B5 Biodegradation. Q Science > QR Microbiology > QR74.8 Bacteria
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Novianti Ika Rachmadani
Date Deposited:	07 Aug 2024 03:56
Last Modified:	07 Aug 2024 03:56
URI:	http://repository.its.ac.id/id/eprint/113342

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