Biodekolorisasi Metil Oranye oleh Uncultured Bacterium Clone I yang Terimobilisasi pada Matriks SA-PVA

Putri, Kamyla (2024) Biodekolorisasi Metil Oranye oleh Uncultured Bacterium Clone I yang Terimobilisasi pada Matriks SA-PVA. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Pengolahan limbah perlu dilakukan sebelum nantinya limbah dibuang ke lingkungan, termasuk badan air seperti sungai, danau, laut, atau tanah. Proses pengolahan bertujuan untuk menghilangkan atau mengurangi polutan berbahaya sehingga limbah yang dibuang tidak merusak ekosistem, tidak mencemari sumber air, dan tidak membahayakan kesehatan manusia serta organisme lainnya. Metil oranye (MO) merupakan salah satu pewarna azo yang banyak digunakan dalam industri tekstil. Pewarna ini sulit untuk dihilangkan dan keberadaannya sangat berbahaya bagi manusia maupun ekosistem perairan karena toksisitasnya yang tinggi sehingga perlu dilakukan pengolahan. Penelitian ini bertujuan untuk mengevaluasi kemampuan bakteri isolat dari lahar Gunung Semeru, Uncultured Bacterium Clone I (JS2), dalam mendegradasi MO. Proses dekolorisasi ini dilakukan menggunakan konsentrasi pewarna MO 10 mg/L dengan metode sel bebas (free cell) dan sel terimobilisasi dalam waktu 10 hari. Imobilisasi dilakukan dengan matriks sodium alginate (SA) dan polyvinyl-alcohol (PVA). Pada metode free cell, hasil penelitian menunjukkan bahwa sel hidup mampu mendegradasi pewarna MO sebesar 41,19%, sementara sel mati hanya sebesar 14,77%. Pada metode imobilisasi digunakan tiga variasi beads: SA-PVA, SA-PVA-JS2 hidup dan SA-PVA-JS2 mati. Hasil menunjukkan bahwa SA-PVA tanpa bakteri hanya mampu mendekolorisasi MO sebesar 25,97%, sedangkan SA-PVA-JS2 hidup mencapai dekolorisasi sebesar 82,16%, dan SA-PVA-JS2 mati hanya sebesar 38,5%. Hal ini menunjukkan bahwa imobilisasi bakteri JS2 dalam matriks SA-PVA secara signifikan meningkatkan efisiensi biodekolorisasi MO karena dapat melindungi bakteri dari zat beracun dan berbahaya serta meningkatkan metabolisme aktivitas bakteri.
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Waste treatment must be carried out before waste is discharged into the environment, including water bodies such as rivers, lakes, seas, or soil. The treatment process aims to eliminate or reduce harmful pollutants so that the discharged waste does not damage ecosystems, contaminate water sources, or pose a threat to human health and other organisms. Methyl orange (MO) is one of the azo dyes widely used in the textile industry. This dye is difficult to remove and its presence is highly hazardous to both humans and aquatic ecosystems due to its high toxicity, therefore, it needs to be processed. This study aimed to evaluate the ability of Uncultured Bacterium Clone I (JS2), an isolate from the lava of Mount Semeru, in degrading MO. The decolorization process was conducted using an MO dye concentration of 10 mg/L with both free cell and immobilized cell methods over a period of 10 days. Immobilization was performed using a sodium alginate (SA) and polyvinyl alcohol (PVA) matrix. In the free cell method, the results showed that live cells were able to decolorize 41.19% of the MO dye, while dead cells only achieved a decolorization of 14.77%. In the immobilization method, three variations of beads were used: SA-PVA, SA-PVA/lived-JS2, and SA-PVA/died-JS2. The results indicated that SA-PVA without bacteria decolorized 25.97% of MO, while SA-PVA/lived-JS2 achieved 82.16% decolorization, and SA-PVA/died-JS2 only achieved 38.5% decolorization. This demonstrates that the immobilization of Uncultured Bacterium Clone I in the SA-PVA matrix significantly enhances the efficiency of MO biodecolorization, as it protects the bacteria from toxic and harmful substances and enhances bacterial metabolic activity.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Biodekolorisasi, Metil Oranye, Uncultured Bacterium Clone I, Imobilisasi, SA-PVA, Biodecolorization, Methyl Orange, Uncultured Bacterium Clone I, Immobilization, SA-PVA
Subjects:	Q Science > QD Chemistry > QD117.S64 Spectrophotometry Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry Q Science > QP Physiology > QP517.B5 Biodegradation. Q Science > QR Microbiology > QR74.8 Bacteria
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Kamyla Putri
Date Deposited:	08 Aug 2024 11:39
Last Modified:	05 Sep 2024 06:54
URI:	http://repository.its.ac.id/id/eprint/113344

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