Pengaruh Variasi Warna Cahaya terhadap Kandungan Senyawa Bioaktif dan Aktivitas Antioksidan Spirulina sp.

Pradani, Anindhita Nabilah (2024) Pengaruh Variasi Warna Cahaya terhadap Kandungan Senyawa Bioaktif dan Aktivitas Antioksidan Spirulina sp. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Spirulina sp. memiliki kandungan antioksidan kuat yang dapat mereduksi radikal
bebas. Pigmen alami pada Spirulina sp. seperti fikosianin dan klorofil dipercaya sebagai sumber
antioksidan tinggi. Cahaya merupakan faktor penting dalam sintesis pigmen tersebut. Tujuan
penelitian ini adalah untuk mengetahui pengaruh variasi warna cahaya merah, biru, putih,
hortisolis dan cahaya matahari terhadap senyawa bioaktif dan aktivitas antioksidan Spirulina
sp. Kultur Spirulina sp. dikultivasi dan dilakukan pemanenan pada fase akhir eksponensial.
Selanjutnya, di ukur aktivitas antioksidan menggunakan metode DPPH, ABTS, dan FRAP.
Selain itu dilakukan perhitungan pigmen klorofil dan perhitungan pigmen fikosianin. Data yang
diperoleh selanjutnya dianalisis menggunakan Anova one way dan dilanjutkan dengan uji post
hoc Tukey test pada taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa perlakuan
variasi warna cahaya memberi pengaruh terhadap kandungan klorofil a, klorofil b, klorofil total,
fikosianin, serta aktivitas antioksidan. Perlakuan cahaya matahari menghasilkan jumlah klorofil
a, klorofil b, klorofil total, dan fikosianin yang paling tinggi. Berdasarkan metode DPPH, IC50
perlakuan variasi warna cahaya dari yang paling tinggi ke paling rendah berturut-turut adalah
LED merah, LED biru, cahaya matahari, LED putih, dan hortisolis. LED cahaya merah
menghasilkan nilai IC50 terbaik sebesar 36,24 mg/l dengan kategori sangat kuat. Berdasarkan
metode ABTS, LED hortisolis dan merah tidak berbeda nyata dalam mempengaruhi aktivitas
antioksidan Spirulina sp. dengan nilai IC50 terbaik sebesar 2,47 mg/l dan 2,20 mg/l kategori
sangat kuat. Sedangkan pada metode FRAP, perlakuan LED hortisolis memiliki hasil uji
tertinggi sebesar 86 mg AAE/g (Ascorbic Acid Equivalent). LED Hortisolis berbeda nyata
dengan LED merah, LED biru, LED putih, dan cahaya matahari. Rekomendasi LED yang dapat
digunakan untuk meningkatkan aktivitas antioksidan dan kandungan klorofil a, klorofil b, serta
klorofil total adalah LED merah atau LED hortisolis. Sedangkan untuk meningkatkan
kandungan fikosianin adalah LED putih.
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The cyanobacterium spirulina contains potent antioxidants that have been demonstrated
to reduce the activity of free radicals. The natural pigments present in spirulina, including
phycocyanin and chlorophyll, are postulated to be a source of high antioxidants. The synthesis
of these pigments is dependent upon light. The objective of this study was to ascertain the
impact of alterations in the hue of red, blue, white, and the presence or absence of sunlight on
the bioactive compounds and antioxidant activity of Spirulina sp. Cultures of Spirulina sp. were
cultivated and harvested during the late exponential phase. Moreover, antioxidant activity was
quantified through the use of three distinct methodologies: DPPH, ABTS, and FRAP.
Furthermore, calculations were performed for the chlorophyll and phycocyanin pigments. The
resulting data were then subjected to analysis using one-way ANOVA, followed by a Tukey
post hoc test at a 95% confidence level. The findings indicated that the light color variation
treatment had an impact on the content of chlorophyll a, chlorophyll b, total chlorophyll,
phycocyanin, and antioxidant activity. The sunlight treatment yielded the highest levels of
chlorophyll a, chlorophyll b, total chlorophyll, and phycocyanin. The IC50 values for the light
color variation treatments, from highest to lowest, were as follows: red LED, blue LED, sunlight,
white LED, and hortisolis. The IC50 value for red light LED was found to be 36.24 mg/L,
representing a very strong category. The ABTS method revealed that the antioxidant activity of
Spirulina sp. was not significantly affected by hortisol or red LEDs, with the best IC50 value
of 2.47 mg/l and 2.20 mg/l, respectively, falling into the very strong category. In the FRAP
method, the LED Hortisolis treatment yielded the highest test result, namely 86 mg AAE/g
(ascorbic acid equivalent). The hortisolis LED treatment exhibited significant differences from
the red LED, blue LED, white LED, and sunlight treatments. LED recommendations that can
be used to increase antioxidant activity and chlorophyll a, chlorophyll b, and total chlorophyll
content are red LED or hortisolis LED. While to increase the content of phycocyanin is white
LED.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Antioksidan, Cahaya, Fikosianin, Klorofil, Spirulina, Antioxidant, Light, Fikocyanin, Chlorophyll, Spirulina
Subjects:	Q Science > QH Biology > QH301 Biology Q Science > QK Botany Q Science > QK Botany > QK568 Microalgae
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Anindhita Nabilah Pradani
Date Deposited:	09 Aug 2024 03:11
Last Modified:	09 Aug 2024 03:11
URI:	http://repository.its.ac.id/id/eprint/113780

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