Identifikasi dan Deteksi Ergosterol selama Pertumbuhan Jamur Kayu Kelapa Sawit (Elaeis guineensis Jacq.)

Setya, Waishaka Galantra (2024) Identifikasi dan Deteksi Ergosterol selama Pertumbuhan Jamur Kayu Kelapa Sawit (Elaeis guineensis Jacq.). Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Beberapa spesies jamur tingkat tinggi mampu menyerang kayu kelapa sawit, bersifat parasit dan mengkolonisasi pohon hidup. Jamur pembusuk kayu dapat diklasifikasikan menjadi jamur busuk lunak, busuk coklat, dan busuk putih, yang masing-masing mendegradasi bahan tanaman berdasarkan kemampuan spesifik spesiesnya untuk menguraikan lignin, selulosa, dan hemiselulosa. Ergosterol merupakan komponen sterol yang dominan pada membran plasma jamur. Sifatnya yang labil membuatnya cepat terdegradasi setelah kerusakan hifa jamur, sehingga memungkinkan untuk digunakan oleh ahli mikrobiologi sebagai indikator biomassa jamur. Pada penelitian ini, dilakukan identifikasi serta penentuan hubungan antara biomassa (berat kering) dengan konsentrasi ergosterol dari beberapa isolat jamur kayu kelapa sawit, yakni GB, PO, JKS2-3, JKS3, dan JKS4. Ekstraksi menggunakan metode Microwave-Assisted Extraction (MAE) dan dilanjutkan dengan pengukuran konsentrasi dengan spektrofotometer UV-Vis). Hasil menunjukkan bahwa GB teridentifikasi sebagai Ganoderma boninense, PO teridentifikasi sebagai Pleurotus ostreatus, JKS2-3 teridentifikasi sebagai Trametes hirsuta, JKS3 teridentifikasi sebagai Pycnoporus sanguineus, dan JKS4 teridentifikasi sebagai Lentinus tigrinus. Semua jamur uji memiliki korelasi kuat antara biomassa (berat kering) dengan konsentrasi ergosterol berdasarkan hasil analisis korelasi Kendall’s tau-b, dengan nilai R2 = 0,854. Hasil tersebut menunjukkan bahwa pola pertumbuhan jamur pada kultur cair berkorelasi dengan konsentrasi ergosterol, sehingga kandungan ergosterol dapat dijadikan dasar untuk mendeteksi biomassa jamur.
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Several species of higher fungi are capable of infecting oil palm wood, acting as parasites and colonizing living trees. Wood rot fungi can be classified into soft rot, brown rot, and white rot fungi, each of which degrades plant material based on its species-specific ability to decompose lignin, cellulose, and hemicellulose. Ergosterol is the dominant sterol component in the fungal plasma membrane. Its labile nature means it is quickly degraded after damage to fungal hyphae, making it possible for microbiologists to use it as an indicator of fungal biomass. In this study, identification and determination of the relationship between biomass (dry weight) and ergosterol concentration were carried out from several oil palm wood fungus isolates, namely GB, PO, JKS2-3, JKS3, and JKS4. Extraction uses the Microwave-Assisted Extraction (MAE) method and is followed by concentration measurement with a UV-Vis spectrophotometer. The results showed that GB isolate was identified as Ganoderma boninense, PO was identified as Pleurotus ostreatus, JKS2-3 was identified as Trametes hirsuta, JKS3 was identified as Pycnoporus sanguineus, and JKS4 was identified as Lentinus tigrinus. All tested mushrooms had a strong correlation between biomass (dry weight) and ergosterol concentration based on the results of Kendall's tau-b correlation analysis, with an R2 value = 0.854. These results indicate that the growth pattern of fungi in liquid culture is correlated with the concentration of ergosterol, so that the ergosterol content can be used as a basis for detecting fungal biomass.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Ergosterol, Identifikasi, Jamur kayu, Kelapa sawit, Spektrofotometer, Ergosterol, Identification, Oil palm, Spectrophotometer, Wood mushroom
Subjects:	Q Science > QH Biology > QH301 Biology
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Waishaka Galantra Setya
Date Deposited:	26 Aug 2024 01:05
Last Modified:	26 Aug 2024 01:05
URI:	http://repository.its.ac.id/id/eprint/114256

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