Sintesis Nanopartikel Perak dan Evaluasi Aktivitas Antioksidan: Optimasi Response Surface Methodology (RSM)

Wulandari, Herlinda Ayu (2024) Sintesis Nanopartikel Perak dan Evaluasi Aktivitas Antioksidan: Optimasi Response Surface Methodology (RSM). Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Sintesis nanopartikel perak ini menggunakan beberapa parameter meliputi konsentrasi asam tartarat, suhu dan waktu sonikasi sehingga untuk mempersingkat waktu dan mengoptimalkan proses sintesis penting dilakukan proses optimasi. Penelitian ini bertujuan untuk mengetahui kondisi optimum sintesis nanopartikel perak berbasis Response Surface Method (RSM)-Face Centered Central Composite Design (F-CCD), serta mengetahui aktivitas nanopartikel perak sebagai antioksidan. Sintesis AgNP meliputi tiga faktor respon yaitu konsentrasi asam tartarat (0,5; 1,0; 1,5 M), waktu sonikasi (20, 40, 60 menit) dan suhu sonikasi (40, 60, 80ºC). Desain penelitian menghasilkan 22 kode sampel yang berbeda. Setelah proses sintesis AgNP yang diukur dengan spektrofotometer UV-Vis. Data absorbansi optimum kemudian diinput dalam RSM yang akan divisualisasikan melalui plot 3D. Kondisi optimum sintesis AgNP dicapai pada konsentrasi asam tartarat 1,0 M, waktu sonikasi 40 menit dengan suhu sonikasi 60ºC. Data yang dianalisis dalam RSM adalah data absrobansi sintesis AgNP dengan waktu pendiaman selama 0 hari dan 8 hari. Hal ini bertujuan untuk mengidentifikasi stabilitas AgNP. Sampel (a) untuk sampel dengan waktu pendiaman selama 0 hari sedangkan (b) sampel dengan waktu pendiaman selama 8 hari. Setelah dilakukan pengamatan dengan waktu selama 8 hari didapatkan nilai absorbansi yang signifikan di antara kedua larutan di mana larutan AgNP dengan penambahan asam tartarat memiliki rata-rata absorbansi yang lebih rendah dibandingkan dengan larutan AgNP tanpa penambahan asam tartarat. Sintesis AgNP dilakukan karakterisasi dengan Particle Size Analyzer (PSA), Transmission Electron Microsope (TEM) dan Fourier Transform Infrared Spectroscopy (FTIR). Hasil pengujian menunjukkan partikel berbentuk spherical dengan ukuran 102,17 nm. Dari hasil FTIR, terlihat adanya asam tartarat yang berperan sebagai capping agent dalam sintesis AgNP disebabkan terdapat adanya interaksi antara gugus fungsional tartarat yaitu gugus karboksilat (gugus C=O dan –OH) dengan permukaan logam perak. Pengujian antioksidan dilakukan dengan metode DPPH menghasilkan inhibisi sebesar 66,9%.
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The synthesis of silver nanoparticles uses several parameters including tartaric acid concentration, temperature and sonication time so that to shorten the time and optimize the synthesis process, an optimization process is important. This study aims to determine the optimum conditions for the synthesis of silver nanoparticles based on the Response Surface Method (RSM)-Face Centered Central Composite Design (F-CCD), and to determine the activity of silver nanoparticles as antioxidants. AgNP synthesis includes three response factors, namely tartaric acid concentration (0.5; 1.0; 1.5 M), sonication time (20, 40, 60 minutes) and sonication temperature (40, 60, 80ºC). The research design produced 22 different sample codes. After the AgNP synthesis process was measured using a UV-Vis spectrophotometer. The optimum absorbance data was then inputted into the RSM which would be visualized through a 3D plot. The optimum conditions for AgNP synthesis were achieved at a tartaric acid concentration of 1.0 M, a sonication time of 40 minutes with a sonication temperature of 60ºC. The data analyzed in RSM is the absorption data of AgNP synthesis with a settling time of 0 days and 8 days. This aims to identify the stability of AgNP. Sample (a) for the sample with a settling time of 0 days while (b) the sample with a settling time of 8 days. After observation for 8 days, a significant absorbance value was obtained between the two solutions where the AgNP solution with the addition of tartaric acid had a lower average absorbance compared to the AgNP solution without the addition of tartaric acid. AgNP synthesis was characterized by Particle Size Analyzer (PSA), Transmission Electron Microsope (TEM) and Fourier Transform Infrared Spectroscopy (FTIR). The test results showed spherical particles with a size of 102.17 nm. From the FTIR results, it can be seen that tartaric acid acts as a capping agent in the synthesis of AgNP due to the interaction between the tartarate functional group, namely the carboxylate group (C = O and -OH groups) with the silver metal surface. Antioxidant testing was carried out using the DPPH method resulting in an inhibition of 66.9%

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Silver Nanoparticles (AgNPs), RSM, Central Composite Design, Antioxidant Activity, Nanopartikel Perak (AgNP), RSM, Central Composite Design, Aktivitas Antioksidan
Subjects:	Q Science Q Science > QD Chemistry Q Science > QD Chemistry > QD75.2 Chemistry, Analytic
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Herlinda Ayu Wulandari
Date Deposited:	11 Aug 2024 08:29
Last Modified:	11 Sep 2024 03:24
URI:	http://repository.its.ac.id/id/eprint/114658

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