Bioaugmentasi Bacillus cereus WN4 Sebagai Penyedia Nitrat Pada Media Tanam Sawi Daging (Brassica rapa L.)

Ilmi, Afifah Nurul (2024) Bioaugmentasi Bacillus cereus WN4 Sebagai Penyedia Nitrat Pada Media Tanam Sawi Daging (Brassica rapa L.). Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Kesuburan tanah sangat mempengaruhi pertumbuhan tanaman, hal ini dikarenakan kandungan nutrisi pada tanah yang mampu membuat tanaman tumbuh dengan baik. Nitrogen diserap oleh akar tanaman dalam bentuk nitrat dan amonium, nitrat sendiri merupakan ion yang digunakan tanaman untuk proses pertumbuhannya. Metode perbaikan kualitas tanah secara biologis dapat dilakukan dengan memanfaatkan mikroorganisme. Bioaugmentasi dengan menambahkan kultur cair berisi mikroorganisme eksogenus pada media tanam dapat meningkatkan populasi mikroorganisme yang telah ada pada media tanam sehingga dapat memperbaiki kualitas tanah, salah satunya dengan meningkatkan kadar nitrat pada tanah. Berdasarkan hasil uji kualitatif, isolat Bacillus cereus WN4 memiliki kemampuan dalam menyediakan nitrat tersedia. Pengambilan data dilakukan dengan melakukan uji kadar nitrat menggunakan metode brusin asam sulfanilat dan pengukuran parameter yang meliputi jumlah daun, tinggi tanaman, dan berat basah tanaman. Data yang terkumpul dianalisis menggunakan metode statistik yaitu Analysis of Variance aplikasi SPSS dan dilanjutkan dengan uji lanjut Tukey taraf α 5%. Hasil penelitian menunjukkan bahwa aplikasi bioaugmentasi Bacillus cereus WN4 berpengaruh terhadap peningkatan konsentrasi nitrat tersedia pada media tanam sawi daging (Brassica rapa L.) dengan kadar nitrat tertinggi ada pada perlakuan T2B3 dengan nitrat tersedia sebesar 19,79 ppm. Selain itu, aplikasi bioaugmentasi Bacillus cereus WN4 pada media tanam Brassica rapa L. berpengaruh terhadap pertumbuhan dan produktivitas tanaman dengan hasil tertinggi ada pada perlakuan T2B1 dengan rata-rata tinggi tanaman 22,33 cm, jumlah daun sebanyak 11,33, dan berat basah sebesar 5,31 gram.
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Soil fertility greatly influences plant growth, this is because the nutrient content in the soil is able to make plants grow well. Nitrogen is absorbed by plant roots in the form of nitrate and ammonium, nitrate itself is an ion that plants use for their growth process. Biological methods of improving soil quality can be done by utilizing microorganisms. Bioaugmentation by adding liquid culture containing exogenous microorganisms to the planting media can increase the population of microorganisms that already exist in the planting media so that it can improve soil quality, one of which is by increasing nitrate levels in the soil. Based on the qualitative test results, the Bacillus cereus WN4 isolate has the ability to provide available nitrate. Data collection was carried out by testing nitrate levels using the sulfanilic acid brusine method and measuring parameters including number of leaves, plant height and plant wet weight. The collected data was analyzed using statistical methods, namely Analysis of Variance SPSS application and continued with the Tukey further test at α 5% level. The results showed that the application of Bacillus cereus WN4 bioaugmentation had an effect on increasing the available nitrate concentration in the pak choy (Brassica rapa L.) planting medium with the highest nitrate levels in the T2B3 treatment with available nitrate of 19.79 ppm. Apart from that, the application of Bacillus cereus WN4 bioaugmentation on the Brassica rapa L. planting medium had an effect on plant growth and productivity with the highest yield in the T2B1 treatment with an average plant height of 22.33 cm, number of leaves of 11.33, and wet weight of 5.31 grams.

Item Type: Thesis (Other)
Uncontrolled Keywords: Bacillus cereus WN4, Bioaugmentasi, Brassica rapa L., Nitrat, Nutrisi.
Subjects: Q Science > QH Biology > QH301 Biology
Divisions: Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User: Afifah Nurul Ilmi
Date Deposited: 13 Aug 2024 06:10
Last Modified: 13 Aug 2024 06:10
URI: http://repository.its.ac.id/id/eprint/115023

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