Ambarita, Agung (2024) Studi Sifat Mekanik, Morfologi, Dan Antibakteri Wound Dressing Berbasis Nanofiber Polivinil Alkohol/Sodium Alginat Dengan Penambahan Minyak Atsiri Daun Sirih Konsentrasi 5 Dan 7,5%. Other thesis, Institut Teknologi Sepuluh November.
![[thumbnail of 02111840000123-Undergraduate_Thesis.pdf]](http://repository.its.ac.id/style/images/fileicons/text.png) |
Text
02111840000123-Undergraduate_Thesis.pdf - Accepted Version Restricted to Repository staff only until 1 October 2026. Download (4MB) | Request a copy |
Abstract
Wound dressing adalah bahan yang digunakan untuk menutupi dan melindungi luka dimana dalam perkembangannya dapat dimanfaatkan untuk memfasilitasi penyembuhan. Umumnya, modern wound dressing memanfaatkan nanofiber sebagai penyusunnya dengan keunggulan rasio luas permukaan terhadap volume sangat tinggi yang mampu meningkatkan efektivitas penyembuhan melalui penyerapan dan pengiriman obat secara optimal. Teknik elektrospinning yang lebih mudah dan praktis dalam memfabrikasi nanofiber merupakan teknik yang populer digunakan. Polyvinyl Alcohol (PVA) dan Sodium Alginate (SA) digunakan sebagai bahan pembuatan wound dressing nanofiber karena memiliki biokompatibilitas tinggi dan kemampuan menyerap eksudat yang baik. Penambahaan minyak atsiri daun sirih yang mengandung senyawa aktif seperti flavonoid dan tanin bertujuan untuk memberikan efek antibakteri dan antiinflamasi. Hasil penelitian sebelumnya menunjukkan bahwa penambahan minyak atsiri daun sirih konsentrasi 1% dan 2% wt belum memiliki sifat antibakteri sehingga dibutuhkan penelitian lebih lanjut dengan memvariasikan konsentrasi minyak atsiri daun sirih.
Pada penelitian ini digunakan komposisi PVA 8% wt, SA 2% wt, dan minyak atsiri daun sirih variasi konsentrasi 0%, 5%, dan 7,5% untuk memfabrikasi nanofiber. Fabrikasi dilakukan dengan menggunakan parameter flow rate 0,5 ml/h, volume 5 ml, jarak nozzle ke collecctor 15 cm, kecepatan putar collector 200 RPM, dan tegangan 18 kV. Uji viskositas terhadap precursor dilakukan sebelum proses fabrikasi nanofiber. Berikutnya dilakukan uji SEM, XRD, dan FTIR untuk menganalisis morfologi berupa diameter ,surface area, derajat kristalinitas, dan senyawa penyusun nanofiber. Yield strength dan modulus Young dievaluasi melalui uji tarik sesuai standar ASTM D638-14, dan zona hambat bakteri diuji dengan metode Kirby-Bauer. Penambahan minyak atsiri daun sirih sebesar 0; 5; dan 7,5wt.% menghasilkan variasi morfologi yang signifikan dimana diameter nanofiber terbesar diperoleh pada konsentrasi 5 % yaitu 419 nm dengan pembentukan beads paling kecil sebesar 6.48% dari total fiber yang dihasilkan. Selain itu, akibat ikatan oksigen dari minyak atsiri daun sirih pada konsentrasi 5 % menghasilkan derajat kristalinitas terbesar sebesar 55%. Namun, zona hambat bakteri diperoleh sebesar 185.92 mm2 dimana konsentrasi 7.5 % menghasilkan zona hambat lebih besar yaitu 218.48 mm2 terhadap bakteri Escherichia coli, serta 71,90 mm2 dan 193,49 mm2 terhadap bakteri Staphylococcus aureus. Dengan demikian, penambahan minyak atsiri daun sirih pada nanofiber PVA-SA sebesar 5% mempunyai potensi untuk dapat dijadikan bahan wound dressing dengan mempertimbangkan juga bahwa nilai modulus Young sebesar 13,08 MPa melebihi batas bawah modulus kulit manusia (5 MPa), dengan nilai Yield strength diperoleh sebesar 0,91 MPa.
====================================================================================================================================
Wound dressing refers to materials used to cover and protect wounds, which have evolved to facilitate healing. Modern wound dressings commonly utilize nanofibers due to their high surface area-to-volume ratio, enhancing healing effectiveness through optimal absorption and drug delivery. Electrospinning, an easier and more practical technique for fabricating nanofibers, is popularly used. Polyvinyl Alcohol (PVA) and Sodium Alginate (SA) are employed in creating nanofiber wound dressings due to their high biocompatibility and excellent exudate absorption capability. The addition of betel leaf essential oil, which contains active compounds such as flavonoids and tannins, aims to provide antibacterial and anti-inflammatory effects. Previous research indicated that the addition of 1% and 2% wt concentrations of betel leaf essential oil did not exhibit antibacterial properties, necessitating further studies with varied concentrations of the essential oil.
In this study, a composition of 8% wt PVA, 2% wt SA, and varying concentrations of betel leaf essential oil (0%, 5%, and 7.5%) was used to fabricate nanofibers. Fabrication was conducted using a flow rate of 0.5 ml/h, a volume of 5 ml, a nozzle-to-collector distance of 15 cm, a collector rotation speed of 200 RPM, and a voltage of 18 kV. Precursor viscosity was tested before the nanofiber fabrication process. Subsequent analyses included SEM, XRD, and FTIR to examine morphology, including diameter, surface area, degree of crystallinity, and constituent compounds of the nanofibers. Yield strength and Young's modulus were evaluated through tensile testing according to ASTM D638-14 standards, and bacterial inhibition zones were tested using the Kirby-Bauer method. The addition of 0%, 5%, and 7.5% wt. of betel leaf essential oil resulted in significant morphological variations, with the largest nanofiber diameter obtained at 5% concentration, measuring 419 nm, and the smallest bead formation at 6.48% of the total fibers produced. Additionally, the oxygen bonds from the 5% concentration of betel leaf essential oil yielded the highest degree of crystallinity at 55%. However, the bacterial inhibition zone was measured at 185.92 mm², while the 7.5% concentration yielded a larger inhibition zone of 218.48 mm² against Escherichia coli, and 71.90 mm² and 193.49 mm² against Staphylococcus aureus. Therefore, the addition of 5% betel leaf essential oil in PVA-SA nanofibers shows potential for wound dressing applications, considering also that the Young's modulus value of 13.08 MPa exceeds the lower limit of human skin modulus (5 MPa), with a yield strength value of 0.91 MPa.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Elektrospinning, PVA/SA, minyak atsiri daun sirih, Wound dressing; Electrospinning, PVA/SA, betel leaf essential oil, wound dressing |
| Subjects: | T Technology > TJ Mechanical engineering and machinery |
| Divisions: | Faculty of Industrial Technology and Systems Engineering (INDSYS) > Mechanical Engineering > 21201-(S1) Undergraduate Thesis |
| Depositing User: | Agung Dwi Silvian Ambarita |
| Date Deposited: | 16 Aug 2024 08:53 |
| Last Modified: | 16 Aug 2024 08:53 |
| URI: | http://repository.its.ac.id/id/eprint/115413 |
Actions (login required)
 |
View Item |