Analisa Pengaruh Panjang Gelombang LED UV Terhadap Pertumbuhan Sel Kanker Darah Pada Metode PDT

Kharismawan, Daniel Anugrah (2024) Analisa Pengaruh Panjang Gelombang LED UV Terhadap Pertumbuhan Sel Kanker Darah Pada Metode PDT. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Terapi fotodinamik, atau Photodynamic Therapy (PDT), merupakan sebuah metoda perawatan, dan pengobatan non-invasif untuk berbagai penyakit, seperti kanker, dan lainnya. PDT bekerja dengan memanfaatkan interaksi antara cahaya, oksigen, dan senyawa peka cahaya atau photosensitizer (PS). Pada perkembangannya, PDT sering digunakan sebagai alternatif dalam pengobatan kanker, karena sifatnya yang non-invasif, yang menyebabkan PDT cenderung lebih aman dibandingkan pilihan-pilihan metode pengobatan kanker lainnya. Salah satu kanker yang cukup umum dijumpai adalah kanker darah, atau leukemia. Maka dari itu, dilakukan penelitian untuk menemukan panjang gelombang yang efektif dalam mengobati kanker darah, dengan menggunakan metode PDT dan senyawa PS berupa tumerone. Digunakan 2 (dua) variasi panjang gelombang, 390-410 nanometer, serta 410-420 nanometer. Penentuan panjang gelombang didapatkan dari penelitian terdahulu mengenai senyawa tumerone sebagai PS. Pengujian dilakukan dengan cara menginjeksi senyawa PS ke dalam microplate yang berisi sel kanker darah, memberikan perlakuan PDT dengan rangkaian yang telah dibuat, dan dilakukan uji MTT(Microtetrazolium) untuk melihat hasil perlakuan. Hasil penelitian yang telah didapatkan menunjukkan nilai IC50 dari 3 sampel perlakuan, Sampel D dimana tidak dilakukan perlakuan PDT, Sampel E dimana dilakukan perlakuan PDT dengan panjang gelombang 390 – 410 nanometer, dan Sampel C dimana dilakukan perlakuan PDT dengan panjang gelombang 410 – 420 nanometer, berturut – turut sebesar 1305,1 μg/mL, 425,0227 μg/mL, 0,000016124 μg/mL. Yang menunjukkan bahwa perlakuan PDT dengan panjang gelombang yang tepat, dapat meningkatkan efektivitas terapi kanker darah secara signifikan.
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Photodynamic Therapy (PDT) is a non-invasive treatment method for various diseases, including cancer. PDT works by utilizing the interaction between light, oxygen, and a photosensitive compound known as a photosensitizer (PS). Over time, PDT has often been used as an alternative cancer treatment due to its non-invasive nature, which tends to make PDT safer compared to other cancer treatment options. One common type of cancer is blood cancer, or leukemia. Therefore, this research was conducted to determine the effective wavelength in treating blood cancer using the PDT method with tumerone as the PS compound. Two wavelength variations were used, 390-410 nanometers and 410-420 nanometers. The determination of these wavelengths was based on previous research on tumerone as a PS compound. The testing was carried out by injecting the PS compound into a microplate containing blood cancer cells, administering PDT treatment with the designed setup, and conducting an MTT (Microtetrazolium) assay to observe the results. The research findings showed the IC50 values of three treatment samples: Sample D, where no PDT treatment was administered; Sample E, where PDT treatment with a wavelength of 390-410 nanometers was administered; and Sample C, where PDT treatment with a wavelength of 410-420 nanometers was administered. The IC50 values were 1305.1 μg/mL, 425.0227 μg/mL, and 0.000016124 μg/mL, respectively. These results indicate that PDT treatment with the appropriate wavelength can significantly enhance the effectiveness of blood cancer therapy.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	leukemia, light emitting diode, photodynamic therapy, wavelength, panjang gelombang
Subjects:	Q Science > QC Physics R Medicine > R Medicine (General) > R857 Biomedical materials--Effect of lasers on R Medicine > RZ Other systems of medicine
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Physics > 45201-(S1) Undergraduate Thesis
Depositing User:	Daniel Anugrah Kharismawan
Date Deposited:	31 Jan 2025 02:22
Last Modified:	31 Jan 2025 02:22
URI:	http://repository.its.ac.id/id/eprint/117214

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