Zakaria, Amiruddin (2025) Optimisasi Proses Ekstraksi Protein dan Modifikasi Protein Menjadi Nano-Protein Melalui Desolvasi dengan Komparasi Pengaruh Crosslinking Agent Glutaraldehid dan Tannic Acid untuk Aplikasi Penghantaran Obat. Masters thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Penelitian ini bertujuan untuk mengoptimalkan proses ekstraksi protein dari dedak padi menggunakan metode Microwave-Assisted Extraction (MAE) dan memodifikasi protein menjadi nanoprotein melalui proses desolvasi. Fokus utama adalah mencari kondisi operasi optimum proses ekstraksi protein dan membandingkan efektivitas agen pengikat silang, yaitu glutaraldehid dan tannic acid, untuk menghasilkan nanopartikel protein yang stabil, biodegradable, dan cocok untuk sistem penghantaran obat (Drug Delivery System, DDS). Dedak padi dipilih karena memiliki banyak neutrical value seperti sifat hipoalergenik, anti cancer serta kelimpahan dan keberlanjutannya. Metode penelitian mencakup optimasi parameter proses MAE, yaitu daya gelombang mikrowave, waktu ekstraksi, pH, dan rasio pelarut terhadap sampel, menggunakan desain komposit sentral (Central Composite Design, CCD). Hasil optimasi menunjukkan bahwa %recovery protein tertinggi (47,76%) diperoleh pada daya 200 watt, waktu 150 detik, pH 12, dan rasio aquades:sampel 5. Proses desolvasi dengan penambahan agen pengikat silang tannic acid menunjukkan hasil karakteristik nanopartikel yang unggul dibandingkan glutaraldehid, dengan ukuran partikel sekitar 157~160 nm, zeta potensial yang stabil (-46 mV) , dan distribusi ukuran partikel cukup homogen (~0.3). Karakterisasi nanopartikel mencakup analisis ukuran partikel, indeks polidispersitas (PDI), zeta potensial, kapasitas penahan air dan minyak (WHC/OHC), serta sifat berbusa dan emulsifikasi. Studi ini mengungkapkan bahwa penggunaan tannic acid, sebagai agen pengikat silang ramah lingkungan, memberikan performa yang kompetitif dan potensial untuk aplikasi DDS yang lebih aman dan efektif.
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This research aims to optimize the protein extraction process from rice bran using the Microwave-Assisted Extraction (MAE) method and modify the protein into nanoproteins through the desolvation process. The main focus is to determine the optimum operating conditions for protein extraction and compare the effectiveness of crosslinking agents, namely glutaraldehyde and tannic acid, in producing stable, biodegradable protein nanoparticles suitable for Drug Delivery Systems (DDS). Rice bran was chosen due to its numerous nutritional values, such as hypoallergenic properties, anti-cancer activity, as well as its abundance and sustainability. The research methodology includes optimizing MAE process parameters, such as microwave power, extraction time, pH, and solvent-to-sample ratio, using Central Composite Design (CCD). The optimization results show that the highest protein recovery (47.76%) was obtained at 200 watts, 150 seconds, pH 12, and a water-to-sample ratio of 5. The desolvation process with tannic acid as a crosslinking agent demonstrated superior nanoparticle characteristics compared to glutaraldehyde, with particle sizes of approximately 157–160 nm, stable zeta potential (-46 mV), and relatively homogeneous particle size distribution (~0.3). Nanoparticle characterization included analysis of particle size, polydispersity index (PDI), zeta potential, water and oil holding capacity (WHC/OHC), as well as foaming and emulsifying properties. This study reveals that the use of tannic acid, as an environmentally friendly crosslinking agent, offers competitive performance and significant potential for safer and more effective DDS applications.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | dedak padi, nanopartikel protein, desolvasi, tannic acid, optimasi ekstraksi |
| Subjects: | R Medicine > R Medicine (General) T Technology > T Technology (General) T Technology > T Technology (General) > TA404 Materials--Biodegradation T Technology > TP Chemical technology > TP1140 Polymers T Technology > TP Chemical technology > TP155.7 Chemical processes. |
| Divisions: | Faculty of Industrial Technology and Systems Engineering (INDSYS) > Chemical Engineering > 24101-(S2) Master Thesis |
| Depositing User: | Amiruddin Zakaria |
| Date Deposited: | 03 Feb 2025 06:12 |
| Last Modified: | 03 Feb 2025 06:12 |
| URI: | http://repository.its.ac.id/id/eprint/117934 |
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