Produksi Lakase oleh Trametes versicolor dan Aplikasinya pada Fabrikasi Water Storage Hydrogel

Yudiargo, Danu Risqi (2025) Produksi Lakase oleh Trametes versicolor dan Aplikasinya pada Fabrikasi Water Storage Hydrogel. Masters thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Lakase adalah enzim dari kelas oksidoreduktase yang memiliki berbagai aplikasi industri salah satunya adalah pembuatan polimer fungsional. Salah satu polimer fungsional yang sangat dibutuhkan di sektor pertanian adalah material penyimpan air (water storage hydrogel) untuk lahan kering. Kombinasi biopolimer lignosulfonat dan kitosan akan memberikan struktur yang sangat larut air untuk sebuah kopolimer water storage hydrogel karena jumlah gugus fungsi hidrofilik yang besar. Penambahan lakase pada fabrikasi kopolimer tersebut akan memberikan efek katalis sempurna dari ikatan kovalen yang terbentuk antara lignosulfonat dan kitosan. Lakase berperan dalam katalisis reaksi oksidasi elektron senyawa aromatik lignosulfonat sehingga peluang berikatan dengan kitosan juga lebih besar. Tujuan dari penelitian ini adalah produksi lakase oleh Trametes versicolor dan aplikasinya dalam fabrikasi water storage hydrogel. Aktivitas crude lakase diukur dengan mengukur oksidasi ABTS. Optimasi fabrikasi hydrogel dilakukan dengan mengukur berat kering hydrogel. Crude lakase yang dihasilkan memiliki aktivitas sebesar 25.847 U/mL dengan titik isoelektrik pH 4. Melalui metode Respons Surface Methodology (RSM) menghasilkan nilai optimum fabrikasi water storage hydrogel yakni pada kandungan lignosulfonat 0,8 g, kitosan 0,4 g, dan volume enzim 500 uL. Daya serap air terbesar pada water storage hydrogel yang berhasil diproduksi adalah sebesar 13,97%.
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Laccase is an enzyme from the oxidoreductase class that has various industrial applications, one of which is the manufacture of functional polymers. One of the functional polymers that is very much needed in the agricultural sector is an air storage material (water storage hydrogel) for dry land. The combination of lignosulfonate and chitosan biopolymers will provide a highly air-soluble structure for a copolymer water storage hydrogel due to the large number of hydrophilic functional groups. The addition of laccase to the copolymer fabrication will provide a perfect catalytic effect from the covalent bond formed between lignosulfonate and chitosan. Laccase plays a role in catalyzing the electron oxidation reaction of compounds that occur in lignosulfonate so that the opportunity to bind to chitosan is also greater. The purpose of this study was to production of laccase by Trametes versicolor and its application in water storage hydrogel fabrication. Crude laccase activity was measured by measuring ABTS oxidation. Optimization of hydrogel fabrication was carried out by measuring the dry weight of the hydrogel. The crude laccase produced has an activity of 25.847 U/mL with an isoelectric point of pH 4. Through the Response Surface Methodology (RSM) method, the optimum value of water storage hydrogel fabrication was obtained at a lignosulfonate content of 0,8 g, chitosan 0,4 g, and an enzyme volume of 500 μL. The largest water absorption capacity of the water storage hydrogel that was successfully produced was 13.97%.

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	Lakase, Hydrogel, Trametes versicolor, Lignosulfonat, Kitosan. Laccase, Hydrogels, Trametes versicolor, Lignosulfonate, Chitosan.
Subjects:	Q Science > QH Biology > QH301 Biology
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46101-(S2) Master Thesis
Depositing User:	Danu Risqi Yudiargo
Date Deposited:	04 Feb 2025 02:56
Last Modified:	04 Feb 2025 02:56
URI:	http://repository.its.ac.id/id/eprint/118041

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