Avicena, Rafi Lingga (2025) Dual Kombinasi Oksidoreduktase Dalam Pre Treatment Limbah Serai (Cymbopogon citratus) Sebagai Substrat Produksi Biogas Menggunakan Sistem Anaerobic Digestion. Other thesis, Institut Teknologi Sepuluh Nopember.
![[thumbnail of 5005211098-Undergraduate_Thesis.pdf]](http://repository.its.ac.id/style/images/fileicons/text.png) |
Text
5005211098-Undergraduate_Thesis.pdf - Accepted Version Restricted to Repository staff only until 1 April 2027. Download (2MB) | Request a copy |
Abstract
Oksidoreduktase adalah kelas enzim yang bekerja berdasarkan reaksi oksidasi-reduksi dengan cara mengambil elektron dari substratnya, dua contoh enzim tersebut yang paling menjajikan dalam aplikasi industri adalah Lakase dan CDH. Tanaman serai (C. citratus) mengandung banyak minyak esensial dan dikomersialkan dalam skala besar. Sisa dari industri minyak atsiri tersebut berpotensi digunakan sebagai sumber substrat biogas. Namun demikian, kandungan anti mikroba dari sisa minyak atsiri masih tinggi dan menyebabkan efektivitas produksi biogas juga menjadi rendah. Oksidoreduktase dapat mengurangi jumlah anti mikroba pada sisa minyak atsiri tersebut melalui reaksi oksidasi enzimatik. Tujuan dari penelitian ini untuk mengetahui pengaruh variasi enzim oksidoreduktase dalam Pre Treatment limbah ekstraksi serai untuk substrat biogas menggunakan sistem Anaerobic Digestion. Enzim yang digunakan diproduksi dari jamur Trametes versicolor dan menunjukan aktivitas Lakase dan CDH masing-masing sebesar 1,586 U/mL dan 0,1233 U/mL. Total protein kedua enzim diukur lalu menunjukan kandungan protein sebesar 0,889 mg/mL dan 0,722 mg/mL untuk masing-masing Lakase dan CDH. Sedangkan titik isoelektrik diperoleh pada Lakase dan CDH masing-masing tercapai pada pH 5 dan 4. Hasil Pre Treatment serai menunjukan penurunan kadar fenol yang paling besar 40% dicapai oleh percobaan kombinasi Lakase dan CDH. Sistem Anaerobic Digestion menghasilkan gas dengan jumlah yang lebih baik dari percobaan kombinasi Lakase dan CDH yaitu sebesar 3,4 mL.
==================================================================================================================================
Oxidoreductase is a class of enzymes that work based on oxidation-reduction reactions by taking electrons from their substrates, two examples of the most promising enzymes in industrial applications are Laccase and CDH. Lemongrass (C. citratus) contains a lot of essential oils and is commercialized on a large scale. The remaining essential oil industry has the potential to be used as a source of biogas substrate. However, the antimicrobial content of the remaining essential oils is still high and causes the effectiveness of biogas production to be low. Oxidoreductase can reduce the amount of antimicrobials in the remaining essential oils through enzymatic oxidation reactions. The purpose of this study was to determine the effect of variations in oxidoreductase enzymes in Pre-Treatment of lemongrass extraction waste for biogas substrates using the Anaerobic Digestion system. The enzymes used are produced from the fungus Trametes versicolor and show Laccase and CDH activities of 1.586 U/mL and 0.1233 U/mL, respectively. The total protein of both enzymes was measured and showed a protein content of 0.889 mg/mL and 0.722 mg/mL for Laccase and CDH respectively. While the isoelectric point obtained in Laccase and CDH was achieved at pH 5 and 4 respectively. The results of the lemongrass Pre Treatment showed the greatest decrease in phenol levels of 40% achieved by the combination of Laccase and CDH. The Anaerobic Digestion system produced gas in a better amount than the combination of Laccase and CDH, which was 3.4 mL
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Anaerobic Digestion, Biogas, Laccase, CDH, Serai |
| Subjects: | Z Bibliography. Library Science. Information Resources > Z665 Library Science. Information Science |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | Rafi Lingga Avicena |
| Date Deposited: | 04 Feb 2025 09:32 |
| Last Modified: | 04 Feb 2025 09:32 |
| URI: | http://repository.its.ac.id/id/eprint/118160 |
Actions (login required)
 |
View Item |