Alkahfi, Muhammad Rayza (2025) Kombinase Lakase dan Selulase Dalam Pre-treatment Serat Sabut Kelapa (Cocos nucifera L.) Sebagai Subtrat Produksi Biogas Menggunakan Sistem Anaerobic Digestion. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Cocos nucifera L. merupakan tanaman yang mengandung lignin sebesar 45,84% dan selulosa sebesar 43,44%. Kandungan selulosa yang cukup tinggi menunjukkan potensi pemanfaatannya sebagai substrat dalam produksi biogas. Namun, keberadaan lignin dalam serat sabut kelapa dapat menghambat sakarifikasi pada proses anaerobic digestion. Selain itu formasi serat selulosa yang masih terikat pada hemiselulosa juga menjadi hambatan dalam proses sakarifikasi. Penggunaan kombinasi dua enzim (Lakase dan Selulase) pada pretreatment diketahui dapat membantu melunakkan serat tanaman. Penelitian ini bertujuan untuk mengetahui pengaruh pre-treatment Lakase dan Selulase terhadap serat sabut kelapa untuk mendapatkan efektifitas produksi biogas. Produksi Lakase dan Selulase dilakukan secara submerged fermentation dilanjutkan dengan pemurnian parsial menggunakan ammonium sulfat pada beberapa fraksi. Karakterisasi enzim berupa kadar protein dan titik isoelektrik masing masing diukur dengan metode kromogenik dan variasi pH. Produksi biogas menggunakan substrat serat sabut kepala yang telah di pretreatment dilakukan dalam skala laboratorium. Hasil penelitian menunjukkan aktivitas tertinggi Lakase dan Selulase masing-masing terjadi pada hari ke 12 (4,64 U/mL) dan hari ke 8 (8,75 U/mL) dengan aktivitas spesifik setelah pemurnian parsial yaitu sebesar 1,56 U/mL dan 12,63 U/mL. Berdasarkan hasil analisis uji One Way ANOVA, diperoleh bahwa perlakuan kombinasi Lakase dan Selulase memberikan pengaruh yang signifikan (p < 0,05) terhadap degradasi lignin rata-rata sebesar 0,22 g yang menyebabkan yield produksi biogas juga tinggi (20,2 mL) dibandingkan dengan perlakuan lainnya. Dapat disimpulkan bahwa pengaruh pretreatment Lakase dan Selulase dapat menurunkan kadar lignin yang berakibat meningkatnya produksi biogas dalam sistem anaerobic digester.
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Cocos nucifera L. is a plant that contains approximately 45.84% lignin and 43.44% cellulose. The relatively high cellulose content indicates its potential utilization as a substrate in biogas production. However, the presence of lignin in coconut coir fibers can inhibit saccharification during the anaerobic digestion process. Additionally, the cellulose fibers are still bound to hemicellulose, which also presents a barrier to saccharification. The use of a combination of two enzymes (Laccase and Cellulase) during pretreatment is known to assist in softening plant fibers. This study aims to investigate the effect of laccase and cellulase pretreatment on coconut coir fibers to improve the efficiency of biogas production. Laccase and Cellulase were produced through submerged fermentation, followed by partial purification using ammonium sulfate at various saturation levels. Enzyme characterization, including protein content and isoelectric point, was conducted using the chromogenic method and pH variation. Biogas production using pretreated coconut coir fiber as the substrate was carried out at laboratory scale. The results showed that the highest activities of Laccase and Cellulase occurred on day 12 (4.64 U/mL) and day 8 (8.75 U/mL), respectively, with specific activities after partial purification of 1.56 U/mL and 12.63 U/mL. Based on the results of a One-Way ANOVA test, the combination treatment of Laccase and Cellulase had a significant effect (p < 0.05) on lignin degradation, averaging 0.22 g, which in turn resulted in a higher biogas yield (20.2 mL) compared to other treatments. It can be concluded that pretreatment with Laccase and Cellulase enzymes effectively reduces lignin content, which leads to an increase in biogas production in an anaerobic digester system.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Anaerobic digestion, Cocos nucifera L, Lakase, Pre-treatment, Selulase, Anaerobic digestion, Laccase, Cellulase |
| Subjects: | Q Science > QH Biology > QH301 Biology Q Science > QR Microbiology T Technology > TP Chemical technology > TP155.7 Chemical processes. T Technology > TP Chemical technology > TP248.3 Biochemical engineering. Bioprocess engineering T Technology > TP Chemical technology > TP248.65.P76 Proteins--Biotechnology. T Technology > TP Chemical technology > TP359 Biogas |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | Muhammad Rayza Alkahfi |
| Date Deposited: | 28 May 2025 08:31 |
| Last Modified: | 28 May 2025 08:31 |
| URI: | http://repository.its.ac.id/id/eprint/119104 |
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