Deteksi Ergosterol Pada Kelapa Sawit (Elaeis guineensis Jacq.) Yang Terinfeksi Ganoderma boninense Penyebab Busuk Pangkal Batang

Aura, Larasati Fahnissa (2025) Deteksi Ergosterol Pada Kelapa Sawit (Elaeis guineensis Jacq.) Yang Terinfeksi Ganoderma boninense Penyebab Busuk Pangkal Batang. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Indonesia termasuk negara produsen kelapa sawit terbesar dengan rata-rata produktivitas 29.28 juta ton Crude Palm Oil (CPO) dan meningkat pada tahun 2017 hingga 34.94 juta ton. Penyakit Busuk Pangkal Batang (BPB) merupakan penyakit yang paling sering menginfeksi tumbuhan kelapa sawit. Penyakit tersebut disebabkan oleh jamur Ganoderma boninense. Deteksi infeksi oleh jamur G. boninense dapat dilakukan dengan salah satu metode yaitu deteksi ergosterol. Ergosterol merupakan bagian komponen dinding sel jamur yang secara eksklusif ditemukan di jamur dan tidak terdapat di organisme lainnya. Penelitian ini bertujuan untuk mengetahui jumlah kandungan ergosterol pada organ akar, batang, dan daun tanaman kelapa sawit kontrol dan yang terinfeksi G. boninense. Deteksi ergosterol dilakukan melalui ekstraksi ergosterol dari sampel tanaman. Setiap organ tanaman akan dilakukan ekstraksi dan diukur absorbansinya menggunakan spektrofotometeer UV-Vis dalam panjang gelombang 282 nm. Hasil analisis ergosterol pada organ akar tanaman kelapa sawit kontrol pada usia 64 hari, 76 hari, 88 hari, dan 109 hari berturut-turut adalah 158.68 ppm, 212.01 ppm, 231.85 ppm, 243.7 ppm. Konsentrasi ergosterol pada organ akar tanaman kelapa sawit dengan aplikasi Ganoderma Rubber Wood Block (GRWB) pada usia 64 hari, 76 hari, 88 hari, dan 109 hari berturut-turut adalah 323.58 ppm, 325.26 ppm, 349.63 ppm, 406.09 ppm. Konsentrasi ergosterol pada organ batang tanaman kelapa sawit kontrol pada usia 64 hari, 76 hari, 88 hari, dan 109 hari berturut-turut adalah 193.39 ppm, 174.28 ppm, 232.3 ppm, 238.96 ppm. Konsentrasi ergosterol pada organ batang tanaman kelapa sawit dengan aplikasi GRWB pada usia 64 hari, 76 hari, 88 hari, dan 109 hari berturut-turut adalah 373.39 ppm, 321.19 ppm, 339,58 ppm, 398.76 ppm. Sedangkan konsentrasi ergosterol pada organ daun tanaman kelapa sawit kontrol pada usia 64 hari, 76 hari, 88 hari, dan 109 hari berturut-turut adalah 165.1 ppm, 174.32 ppm, 215.14 ppm, 191.89 ppm. Konsentrasi ergosterol pada organ daun tanaman kelapa sawit dengan aplikasi GRWB pada usia 64 hari, 76 hari, 88 hari, dan 109 hari berturut-turut adalah 308.72 ppm, 379.67 ppm, 359.71 ppm, 357.82 ppm.
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Indonesia is one of the largest palm oil producing countries with an average productivity of 29.28 million tons of Crude Palm Oil (CPO) and increased in 2017 to 34.94 million tons. Basal Stem Rot (BSR) disease is the disease that most often infects oil palm plants. The disease is caused by the fungus Ganoderma boninense. Detection of infection by the fungus G. boninense can be done by one method, namely ergosterol detection. Ergosterol is a component of the fungal cell wall that is exclusively found in fungi and not found in other organisms. This study aims to determine the amount of ergosterol content in the roots, stems, and leaves of control oil palm plants and those infected with G. boninense. Ergosterol detection is carried out through ergosterol extraction from plant samples. Each plant organ will be extracted and its absorbance measured using a UV-Vis spectrophotometer at a wavelength of 282 nm. The results of ergosterol analysis in the root organs of control oil palm plants at the ages of 64 days, 76 days, 88 days, and 109 days were 158.68 ppm, 212.01 ppm, 231.85 ppm, 243.7 ppm, respectively. The concentration of ergosterol in the root organs of oil palm plants with Ganoderma Rubber Wood Block (GRWB) application at the ages of 64 days, 76 days, 88 days, and 109 days were 323.58 ppm, 325.26 ppm, 349.63 ppm, 406.09 ppm, respectively. The concentration of ergosterol in the stem organs of control oil palm plants at the ages of 64 days, 76 days, 88 days, and 109 days were 193.39 ppm, 174.28 ppm, 232.3 ppm, 238.96 ppm, respectively. The concentration of ergosterol in the stem organs of oil palm plants with GRWB application at the ages of 64 days, 76 days, 88 days, and 109 days were 373.39 ppm, 321.19 ppm, 339.58 ppm, 398.76 ppm, respectively. While the concentration of ergosterol in the leaf organs of control oil palm plants at the ages of 64 days, 76 days, 88 days, and 109 days were 165.1 ppm, 174.32 ppm, 215.14 ppm, 191.89 ppm, respectively. The concentration of ergosterol in oil palm leaf organs with GRWB application at the ages of 64 days, 76 days, 88 days, and 109 days were 308.72 ppm, 379.67 ppm, 359.71 ppm, 357.82 ppm, respectively.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	BPB, Ergosterol, G. boninense, Kelapa Sawit BSR, Ergosterol, G. boninense, Palm oil
Subjects:	Q Science > QH Biology > QH301 Biology
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Larasati Fahnissa Aura
Date Deposited:	14 Jul 2025 04:06
Last Modified:	14 Jul 2025 04:06
URI:	http://repository.its.ac.id/id/eprint/119618

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