Potensi Antikanker Nanopartikel Perak Biogenik Dari Ekstrak Daun Dewa (Gynura Pseudochina) Pada Lini Sel Kanker Payudara (T47D)

Mariroh, Azzah Laichatul (2025) Potensi Antikanker Nanopartikel Perak Biogenik Dari Ekstrak Daun Dewa (Gynura Pseudochina) Pada Lini Sel Kanker Payudara (T47D). Masters thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Kanker payudara merupakan penyakit dengan prevalensi tertinggi pada wanita. Sel kanker T47D sebagai salah satu model sel kanker payudara dengan reseptor estrogen positif (ER+) dan progesteron positif (PR+) menunjukkan proliferasi yang lebih tinggi dibandingkan dengan MCF-7 sehingga menuntut pencarian terapi alternatif yang lebih efektif. Nanoteknologi, khususnya nanopartikel perak (AgNPs), menjadi pilihan potensial sebagai terapi antikanker karena kemampuan toksisitasnya terhadap sel kanker. Sintesis AgNPs menggunakan metode biologi yang memanfaatkan tanaman obat telah menarik perhatian sebagai alternatif yang lebih ramah lingkungan dan ekonomis. Salah satu tanaman potensial adalah Gynura pseudochina (L.) DC., yang mengandung senyawa bioaktif seperti flavonoid, alkaloid, dan tanin dengan efek antikanker. AgNPs dari G. pseudochina dikarakterisasi menggunakan Uv-Vis Spectrophotometer dan Transmission Electron Microscopy (TEM) dan menunjukkan hasil masing masing pada rentang 440 nm dengan ukuran 17-35 nm. Efektivitas AgNPs terhadap sel T47D diamati melalui uji sitotoksisitas, apoptosis, serta ekspresi gen C-myc, Cyclin D, dan Apaf-1. Berdasarkan penelitian yang telah dilakukan, AgNPs Gynura pseudochina dengan ukuran 17-35 nm menunjukkan potensi sebagai agen antikanker terhadap sel kanker payudara (T47D). AgNPs Gynura pseudochina terbukti mampu menurunkan viabilitas T47D dengan nilai IC50 sebesar 20 µg/ml inkubasi 24 jam dan dikategorikan sangat toksik. AgNPs Gynura pseudochina juga mampu menginduksi apoptosis T47D dengan persentase tertinggi pada konsentrasi 40 µg/ml sekitar 99,5%. AgNPs Gynura pseudochina dapat menurunkan ekspresi gen c-Myc dengan penurunan tertinggi pada kosentrasi 40 µg/ml yaitu sebesar 0,820 fold change sedangkan, penurunan terendah pada AgNPs Gynura pseudochin konsentrasi 10 µg/ml yaitu sebesar 0,680 fold change. Selain c-myc, AgNPs Gynura pseudochina juga menurunkan ekspresi gen cyclin-D, dengan penurunan tertinggi pada kosentrasi 40 µg/ml yaitu sebesar 0,820 fold change sedangkan, penurunan terendah pada AgNPs Gynura pseudochina konsentrasi 10 µg/ml yaitu sebesar 0,680 fold change. Untuk ekspresi gen Apaf-1 mengalami peningktan tertinggi dari perlakuan AgNPs Gynura pseudochina pada konsentrasi 40 µg/ml yaitu sebesar 0,606 fold change. Sementara peningkatan terendah terdapat pada AgNPs Gynura pseudochina konsentrasi 10 µg/ml yaitu sebesar 0,540 fold change.
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Breast cancer is a disease with the highest prevalence in women. T47D cancer cells as one of the breast cancer cell models with positive estrogen receptors (ER+) and positive
progesterone (PR+) show higher proliferation compared to MCF-7, thus requiring the search for more effective alternative therapies. Nanotechnology, especially silver nanoparticles (AgNPs), is a potential choice as an anticancer therapy because of its toxicity to cancer cells.
The synthesis of AgNPs using biological methods that utilize medicinal plants has attracted attention as a more environmentally friendly and economical alternative. One of the potential plants is Gynura pseudochina (L.) DC., which contains bioactive compounds such as flavonoids, alkaloids, and tannins with anticancer effects. In this study, AgNPs from G. pseudochina were synthesized and characterized using Uv-Vis Spectrophotometer and Transmission Electron Microscopy (TEM) to ensure their feasibility in application to T47D cells. The effectiveness of AgNPs on T47D cells was observed through cytotoxicity, apoptosis, and C-myc, Cyclin D, and Apaf-1 gene expression tests. Based on the research that has been done, AgNPs Gynura pseudochina with a size of 17-35 nm shows potential as an anticancer agent against breast cancer cells (T47D). AgNPs Gynura pseudochina have been proven to be able to reduce the viability of T47D with an IC50 value of 20 µg/ml of 24-hour incubation and are categorized as highly toxic. AgNPs Gynura pseudochina are also able to induce apoptosis of T47D with the highest percentage at a concentration of 40 µg/ml around 99.5%. AgNPs Gynura pseudochina can reduce c-Myc gene expression with the highest decrease at a concentration of 40 µg/ml which is 0.820 fold change while the lowest decrease
in AgNPs Gynura pseudochina concentration of 10 µg/ml is 0.680 fold change. In addition to c-myc, Gynura pseudochina AgNPs also decreased cyclin-D gene expression, with the highest decrease at a concentration of 40 µg/ml, which was 0.820 fold change, while the lowest decrease was at AgNPs Gynura pseudochina concentration of 10 µg/ml, which was 0.680 fold change. For Apaf-1 gene expression, there was the highest increase from AgNPs Gynura pseudochina treatment at a concentration of 40 µg/ml, which was 0.606 fold change. While the lowest increase was found in AgNPs Gynura pseudochina concentration of 10 µg/ml, which was 0.540 fold change.

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	AgNPs, Antikanker, Gynura pseudochina, Kanker Payudara, T47, AgNPs, Anticancer, Breast Cancer, Gynura pseudochina, T47
Subjects:	Q Science > QH Biology > QH301 Biology Q Science > QR Microbiology > QR 201.T84 Tumors. Cancer
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46101-(S2) Master Thesis
Depositing User:	Azzah Laichatul Mariroh
Date Deposited:	15 Jul 2025 03:17
Last Modified:	15 Jul 2025 03:17
URI:	http://repository.its.ac.id/id/eprint/119758

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