Potensi Protista Thraustochytrid AM2 B Penghasil Omega-3 Dari Ekosistem Mangrove Wonorejo Sebagai Alternatif Bahan Aditif Pelet Pakan Ikan

Alifia, Luluk (2025) Potensi Protista Thraustochytrid AM2 B Penghasil Omega-3 Dari Ekosistem Mangrove Wonorejo Sebagai Alternatif Bahan Aditif Pelet Pakan Ikan. Masters thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Omega-3 seperti DHA (Docosahexaenoic Acid) dan EPA (Eicosapentaenoic Acid) merupakan asam lemak esensial yang berperan untuk perkembangan organ dan jaringan saraf makhluk hidup. Sumber utama Omega-3 saat ini dari ikan laut seperti salmon, namun penangkapan secara berlebihan dapat mengganggu kelestariannya. Thraustochytrid adalah protista yang dapat mengakumulasi Omega-3 hingga 20% dari biomassa, sehingga menjadi sumber alternatif yang potensial. Untuk menghasilkan biomassa dengan kandungan lipid tinggi, diperlukan proses kultivasi yang tepat dengan kondisi medium yang sesuai. Limbah tahu berpotensi menjadi medium kultivasi alternatif yang ekonomis karena kaya nutrisi. Penelitian ini bertujuan untuk mengetahui pengaruh pH, salinitas, dan limbah tahu dalam optimasi kultivasi terhadap pertumbuhan sel, kadar DHA dan EPA, serta potensi biomassa protista sebagai bahan aditif pelet pakan ikan. Tahapan penelitian meliputi peremajaan isolat, pembuatan seed culture, skrining isolat penghasil Omega-3, optimasi kultivasi dengan variasi pH, salinitas, dan konsentrasi limbah tahu dengan RSM (Response Surface Methodology), serta pengukuran kadar DHA dan EPA isolat hasil optimasi. Selanjutnya dilakukan kultivasi untuk aplikasi pelet pakan ikan dengan dosis biomassa 10³, 10⁶, dan 10⁹ sel/mL, pengukuran kadar DHA dan EPA pada pelet serta analisis data menggunakan uji statistika ANOVA Two Way. Penelitian ini menggunakan protista isolat AM2 B dengan kadar relatif Omega-3 tertinggi untuk optimasi kultivasi menggunakan RSM (Response Surface Methodology). Hasil optimasi menunjukkan bahwa pada pH 7, salinitas 15 ppt, dan penambahan 50% v/v limbah tahu, biomassa yang semula 4,070 g/L menurun menjadi 2,816 g/L dengan Optical Density sebesar 2,266. Kadar DHA meningkat dari 10,41 mg/g menjadi 39,06 mg/g, sedangkan kadar EPA mengalami penurunan dari 83,47 mg/g menjadi 8,11 mg/g. Aplikasi biomassa pada pelet menunjukkan penurunan kadar DHA dan EPA sebesar 27,24–58,78% selama 30 hari penyimpanan, dengan kadar akhir 11,45–23,65 g/kg.
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Omega-3 fatty acids, such as DHA (Docosahexaenoic Acid) and EPA (Eicosapentaenoic Acid), are essential fatty acids that play a role in the development of organs and neural tissues in living organisms. Currently, the main sources of
Omega-3 are marine fish such as salmon; however, overfishing may threaten their sustainability. Thraustochytrids are protists capable of accumulating Omega-3 up to 20% of their biomass, making them a potential alternative source. To produce biomass with high lipid content, appropriate cultivation processes with suitable medium conditions are required. Tofu wastewater has the potential to serve as an
economical alternative cultivation medium due to its nutrient-rich composition. This study aims to determine the effects of pH, salinity, and tofu wastewater in
cultivation optimization on cell growth, DHA and EPA content, and the potential of protist biomass as an additive for fish feed pellets. The research stages included
isolate rejuvenation, seed culture preparation, screening of Omega-3-producing isolates, cultivation optimization using variations of pH, salinity, and tofu wastewater concentration with Response Surface Methodology (RSM), and
measurement of DHA and EPA levels from optimized isolates. Subsequently, cultivation was applied for fish feed pellets using biomass doses of 10³, 10⁶, and 10⁹ cells/mL, with measurements of DHA and EPA levels in the pellets, and data analysis performed using Two-Way ANOVA. This study utilized the AM2 B protist isolate with the highest relative Omega-3 content for cultivation optimization using RSM. The optimization results showed that at pH 7, salinity of 15 ppt, and the addition of 50% v/v tofu wastewater, the biomass decreased from 4,070 g/L to 2,816 g/L with an Optical Density of 2,266. The DHA content increased from 10,41 mg/g to 39,06 mg/g, while the EPA content decreased from 83,47 mg/g to 8,11 mg/g. The application of biomass to the pellets showed a reduction in DHA and EPA levels by 27,24–58,78% over 30 days of storage, with final levels ranging from 11,45 to 23,65 g/kg.

Item Type:	Thesis (Masters)
Uncontrolled Keywords:	Omega-3, Protista Thraustochytrid, Pakan Ikan, dan Response Surface Methodology. Fish Feed, Omega-3, Protist Thraustochytrid, dan Response Surface Methodology.
Subjects:	Q Science > QH Biology > QH301 Biology Q Science > QR Microbiology S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46101-(S2) Master Thesis
Depositing User:	Luluk Alifia
Date Deposited:	23 Jul 2025 03:13
Last Modified:	23 Jul 2025 03:13
URI:	http://repository.its.ac.id/id/eprint/120063

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