Pretreatment Selulase Dan Mannanase Pada Biomassa Alga Hijau (Ulva lactuca) Sebagai Substrat Produksi Biogas Menggunakan Sistem Anaerobic Digestion

Situmorang, Mico Batara Tohab (2025) Pretreatment Selulase Dan Mannanase Pada Biomassa Alga Hijau (Ulva lactuca) Sebagai Substrat Produksi Biogas Menggunakan Sistem Anaerobic Digestion. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Alga hijau (Ulva lactuca) memiliki komposisi selulosa sebanyak 30,1% dan 21,5% hemiselulosa. Selulosa dan hemiselulosa (terutama mannan) dapat menjadi sumber energi bila terjadi reaksi sakarifikasi, sehingga mikroba mampu memanfaatkannya menjadi biogas. Proses sakarifikasi bisa dilakukan dengan pemberian enzim tertentu. Proses sakarifikasi selulosa memerlukan selulase, yang terdiri dari endo-β-1,4-glukanase dan exo-β-1,4-glukanase yang memotong ikatan glikosidik selobiosa menjadi glukosa, sedangkan pada sakarifikasi mannan, mannanase mengikat substrat mannan dan memutus ikatan glikosidik β-(1,4) atau α-(1,6) antar unit manosa melalui kerja endo-β-mannanase dan exo-β-mannanase. Penelitian ini bertujuan untuk mengetahui pengaruh sakarifikasi biomassa Ulva lactuca menggunakan enzim selulase dan mannanase dalam produksi biogas dalam sistem anaerobic digestion. Penelitian ini mengamati hasil produksi biogas dengan marka volume gas dan cairan serta kadar gula pereduksi dengan metode 3,5-Dinitrosalicylic acid (DNS). Dari pretreatment yang dilakukan, kadar gula pereduksi dan biogas diukur dan dibandingkan. Hasil penelitian menunjukkan bahwa kombinasi enzim selulase dan mannanase dengan urutan pemberian selulase terlebih dahulu menghasilkan volume biogas tertinggi sebesar 177 mL dan kadar gula pereduksi sebesar 130,27 mg/mL, mengindikasikan efektivitas kombinasi enzim dalam meningkatkan efisiensi hidrolisis dan konversi biomassa menjadi biogas.
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The green alga (Ulva lactuca) contains 30.1% cellulose and 21.5% hemicellulose. Cellulose and hemicellulose (primarily mannan) can serve as energy sources through saccharification, enabling microorganisms to convert them into biogas. The saccharification process can be facilitated by the addition of specific enzymes. Cellulose saccharification requires cellulase, which consists of endo-β-1,4-glucanase and exo-β-1,4-glucanase that cleave the glycosidic bonds in cellobiose into glucose. In contrast, mannan saccharification involves mannanase binding to the mannan substrate and breaking the β-(1,4) or α-(1,6) glycosidic bonds between mannose units through the action of endo-β-mannanase and exo-β-mannanase. This study aimed to investigate the effect of saccharification of Ulva lactuca biomass using cellulase and mannanase enzymes on biogas production in an anaerobic digestion system. The study evaluated biogas output by measuring gas and liquid volumes and reducing sugar content using the 3,5-Dinitrosalicylic acid (DNS) method. Based on the pretreatment process, reducing sugar levels and biogas volume were measured and compared. The results showed that the combination of cellulase and mannanase enzymes, with cellulase applied first, produced the highest biogas volume of 177 mL and a reducing sugar concentration of 130.27 mg/mL, indicating the effectiveness of the enzyme combination in enhancing hydrolysis efficiency and biomass conversion into biogas.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Anaerobic digestion, Biogas, Mannanase, Selulase, Ulva lactuca Anaerobic Digestion, Biogas, Cellulase, Mannanase, Ulva lactuca
Subjects:	Q Science > QP Physiology > QP609.P78 Proteolytic enzymes.
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis
Depositing User:	Mico Batara Tohab Situmorang
Date Deposited:	23 Jul 2025 00:57
Last Modified:	23 Jul 2025 00:57
URI:	http://repository.its.ac.id/id/eprint/120584

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