Sa'diyah, Risya Atus (2025) Pengaruh Kombinasi Kitosan dan Trichoderma spp. terhadap Pertumbuhan Jamur Ganoderma boninense. Other thesis, Institut Teknologi Sepuluh Nopember.
![[thumbnail of 5005211025-Undergraduate_Thesis.pdf]](http://repository.its.ac.id/style/images/fileicons/text.png) |
Text
5005211025-Undergraduate_Thesis.pdf - Accepted Version Restricted to Repository staff only Download (5MB) | Request a copy |
Abstract
Ganoderma boninense merupakan salah satu jenis jamur patogen yang dapat menyebaban busuk pangkal batang pada kelapa sawit. Upaya pengendalian jamur tersebut sudah banyak
dilakukan melalui agen biologi, kimia, dan praktik budidaya. Jamur Trichoderma spp. merupakan salah satu jamur antagonis yang menghasilkan enzim kitinase dan glukanase.Enzim tersebut dapat merusak dinding sel G. boninense. Selain itu, kitosan juga diketahui sebagai antifungi karena bersifat polikationik (memiliki banyak muatan positif) yang dapat
berikatan dengan membran sel jamur yang bermuatan negatif sehingga menghambat pertumbuhan miselia jamur, pembentukan dan perkembangan spora. Oleh karena itu, penelitian
ini bertujuan untuk mengetahui pengaruh kitosan dengan variasi konsentrasi 0,25% 0,5%; 0,75%; 1,00%; 1,25%: 1,50% (w/v) terhadap pertumbuhan jamur Ganoderma boninense secara
kultur tunggal maupun kombinasi dengan Trichoderma spp. Hasil penelitian menunjukkan bahwa perlakuan kitosan terhadap jamur G. boninense dan Trichoderma spp. memiliki trend profil pertumbuhan diameter yang sama yaitu semakin meningkat. Perlakuan kombinasi kitosan dan Trichoderma spp. menunjukkan efek penghambatan dari hari ke-1 hingga ke-7 terhadap jamur G. boninense. Perlakuan kitosan pada G. boninense secara mikroskopis tampak adanya pengerutan, penggumpalan, hingga lisis hifa. Pada Trichoderma spp., secara mikroskopis terlihat hifa mengkerut disertai peningkatan jumlah spora. Kombinasi kitosan dan Trichoderma
spp. secara mikroskopis tampak penempelan hifa Trichoderma spp. pada hifa G. boninense.
========================================================================================================================================
Ganoderma boninense is a pathogenic fungus known to cause basal stem rot in oil palm. Various control efforts have been undertaken using biological agents, chemical treatments, and cultivation practices. Trichoderma spp. is one of the antagonistic fungi capable of producing
chitinase and glucanase enzymes, which degrade the cell wall of G. boninense. In addition, chitosan is recognized for its antifungal properties due to its polycationic nature (possessing multiple positive charges), allowing it to interact with the negatively charged fungal cell membranes, thereby inhibiting mycelial growth, spore formation, and development. This study aimed to evaluate the effect of chitosan at varying concentrations (0.25%, 0.5%, 0.75%, 1.00%, 1.25%, and 1.50% w/v) on the growth of G. boninense in both single and dual cultures with Trichoderma spp. The results of the study showed that chitosan treatment on G. boninense and Trichoderma spp. exhibited a similar trend in diameter growth profiles. The combined treatment of chitosan and Trichoderma spp. demonstrated an inhibitory effect from day 1 to day 7 on G. boninense. Microscopically, chitosan treatment on G. boninense showed hyphal shrinkage,clumping, and lysis. In Trichoderma spp., microscopic observations revealed hyphal shrinkage accompanied by an increase in spore production. The combination of chitosan and Trichoderma spp. microscopically showed attachment of Trichoderma spp. hyphae to G. boninense hyphae.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Antifungi, Ganoderma boninense, Kitosan, Patogen, Trichoderma spp.,Antifungal, Chitosan, Ganoderma boninense, Pathogen, Trichoderma spp. Antifungal, Chitosan, Ganoderma boninense, Pathogen, Trichoderma spp. |
| Subjects: | Q Science Q Science > QH Biology Q Science > QR Microbiology |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | Risya Atus Sa'diyah |
| Date Deposited: | 29 Jul 2025 01:15 |
| Last Modified: | 29 Jul 2025 01:15 |
| URI: | http://repository.its.ac.id/id/eprint/122356 |
Actions (login required)
 |
View Item |