Sejati, Adimas Waloya (2025) Pengaruh Aplikasi Alginate Hidrogel Bovine Hydroxyapatite Terhadap Penyembuhan Luka Kulit Mencit (Mus musculus) Berdasarkan Pengamatan Diameter Luka dan Histologis. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Luka merupakan kerusakan jaringan kulit yang dapat memicu mikroorganisme patogen dan memperlambat proses penyembuhan. Salah satu pendekatan yang banyak dikembangkan penyembuhan luka adalah penggunaan biomaterial berbasis bahan alami. Alginate-hydroxyapatite Alg-BHA, yang berbahan baku alga coklat dan tulag sapi, diketahui memiliki sifat bioaktif dan biokompatibel sehingga berpotensi untuk menjadi biomaterial untuk mempercepat penyembuhan luka (Wound healing). Penelitian ini bertujuan untuk mengetahui pengaruh aplikasi Alg-BHA terhadap percepatan penyembuhan luka kulit mencit (M. musculus) melalui analisis makroskopis diameter luka serta analisis histologis secara kualitatif, kuantitatif, dan semi-kuantitatif. Luka eksisi berdiameter 4 mm dibuat pada punggung mencit, yang kemudian dibagi menjadi tiga kelompok diantaranya yakni kontrol K0, perlakuan Alg-BHA dengan interval 4 hari sekali K1, dan 6 hari sekali K2. Morfologi luka diamati setiap dua hari selama 14 hari, sementara jaringan luka dianalisis secara histologis pada hari ke-15 menggunakan pewarnaan hematoksilin-eosin (H&E). Hasil menunjukkan bahwa perlakuan Alg-BHA mempercepat penutupan luka secara signifikan (p<0,05) dibanding kontrol pada fase inflamasi, proliferasi, hingga remodelling. Analisis histologi menunjukkan peningkatan ketebalan epitel (30,55–31,2 µm), densitas fibroblas (3734,78–4139,89 sel), dan skor semi-kuantitatif serat kolagen (13,3–13,7) pada kelompok perlakuan. Tidak terdapat perbedaan signifikan antara K1 dan K2, yang menunjukkan efektivitas Alg-BHA relatif stabil pada kedua interval pemberian. Secara umum, Alg-BHA mempercepat penyembuhan luka melalui stimulasi aktivitas fibroblas, peningkatan angiogenesis, dan pematangan serat kolagen. Kombinasi ion kalsium dari HAp dan lingkungan lembap dari alginat turut mendukung proses re-epitelisasi. Dengan demikian, Alg-BHA berpotensi menjadi biomaterial yang efektif dalam terapi penyembuhan luka kulit.
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Wounds are disruptions of skin tissue that can serve as entry points for pathogenic microorganisms and delay the healing process. One promising approach in wound treatment is the use of natural-based biomaterials. Alginate-hydroxyapatite (Alg-BHA), synthesized from brown algae and bovine bone, has demonstrated bioactive and biocompatible properties, making it a potential candidate to accelerate wound healing. This study aimed to evaluate the effect of Alg-BHA application on skin wound healing in mice (Mus musculus) through macroscopic analysis of wound diameter and histological assessments, including qualitative, quantitative, and semi-quantitative evaluations. Full-thickness excisional wounds (4 mm in diameter) were created on the dorsal area of mice and divided into three groups: control (K0), Alg-BHA treatment with 4-day intervals (K1), and 6-day intervals (K2). Wound morpholgy was monitored every two days for 14 days, and tissue samples were collected on day 15 for histological analysis using hematoxylin and eosin (H&E) staining. Results indicated that AlgBHA treatment significantly enhanced wound closure (p < 0.05) compared to the control group, across inflammatory, proliferative, and remodelling phases. Histological analysis revealed increased epithelial thickness (30.55–31.2 µm), fibroblast density (3734.78–4139.89 cells), and semi- quantitative collagen fiber scores (13.3–13.7) in treated groups. No significant differences were observed between K1 and K2, suggesting consistent efficacy of Alg-BHA across both treatment intervals. Overall, Alg-BHA accelerated wound healing by promoting fibroblast activity, enhancing angiogenesis, and facilitating collagen maturation. The synergistic effects of calcium ions from hydroxyapatite and the moist environment provided by alginate supported
the re-epithelialization process. These findings indicate that Alg-BHA holds considerable potential as an effective biomaterial for skin wound therapy.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Alginate Hydroxyapatite Alg-BHA, Histologi, ImageJ, Mus musculus, Penyembuhan luka Alginate Hydroxyapatite Alg-BHA, Histology, ImageJ, Mus musculus, Wound healing |
| Subjects: | Q Science Q Science > QH Biology Q Science > QL Zoology |
| Divisions: | Faculty of Science and Data Analytics (SCIENTICS) > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | Adimas Waloya Sejati |
| Date Deposited: | 29 Jul 2025 06:22 |
| Last Modified: | 29 Jul 2025 06:22 |
| URI: | http://repository.its.ac.id/id/eprint/122580 |
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