Putra, Arda Fridua (2025) MWCNT Screen-Printed Electrode Termodifikasi Ag/Pt dan Au/Pt untuk Sistem Deteksi Phytol, Quinoline, dan Stigmasterol pada Kelapa Sawit Terinfeksi Ganoderma boninense. Masters thesis, Institut Teknologi Sepuluh Nopember.
![[thumbnail of 6009231013-Master_Thesis.pdf]](http://repository.its.ac.id/style/images/fileicons/text.png) |
Text
6009231013-Master_Thesis.pdf - Accepted Version Restricted to Repository staff only Download (7MB) | Request a copy |
Abstract
Minyak kelapa sawit banyak diproduksi di Indonesia dan Malaysia hingga mencapai 84% dari produksi global. Namun terdapat potensi kerugian mencapai 500 juta USD tiap tahunnya dikarenakan jamur Ganoderma boninense. Perkebunan sawit di Indonesia mengalami persebaran infeksi hingga 45%, sehingga diperlukan pemantauan dini secara intensif untuk mengurangi persebaran jamur tersebut. Metode yang dikembangkan adalah melalui pengujian elektrokimia dengan screen-printed electrode (SPE) terhadap senyawa metabolit sekunder yang dihasilkan tumbuhan ketika terinfeksi jamur, meliputi phytol, quinoline, dan stigmasterol. SPE difabrikasi dengan menggunakan tinta karbon konduktif pada kertas stiker transparan. Untuk meningkatkan performa SPE, dilakukan modifikasi dengan nanokomposit MWCNT-Ag/Pt dan MWCNT-Au/Pt. Nanopartikel Ag (5 mM) dan Pt (2,5; 5; dan 10 mM) disintesis dengan metode hidrotermal sedangkan Au (5 mM) disintesis dengan reduksi sitrat. Nanokomposit dibentuk dengan metode sonikasi dan didepositkan pada SPE dengan drop-casting. Nanopartikel dan nanokomposit dievaluasi dengan karakterisasi spektroskopi UV-vis, FTIR, XPS, serta XRD, sedangkan SPE dievaluasi dengan SEM, hidrofobisitas, four-point probe, CV, serta EIS. Pengujian senyawa metabolit sekunder dilakukan dengan DPV serta kronoamperometri dengan elektrolit 0.1 M PBS. Berdasarkan karakterisasi terhadap nanopartikel dan SPE, nanokomposit berhasil dibentuk dengan sonikasi nanopartikel dan MWCNT serta dideposisi pada SPE dengan metode drop-casting. Berdasarkan karakterisasi elektrokimia, diperoleh variasi terbaik untuk modifikasi SPE adalah nanokomposit dengan rasio 3:1 dan konsentrasi Pt 5 mM baik pada MWCNT-Ag/Pt maupun MWCNT-Au/Pt. Berdasarkan pengujian berulang pada SPE, SPE memiliki stabilitas yang baik meskipun difabrikasi dengan konsep sekali pakai. Deteksi terbaik dengan DPV diperoleh oleh SPE termodifikasi MWCNT-Au/Pt terhadap phytol, quinoline dan stigmasterol dengan R2 dan LOD secara berturut-turut diperoleh sebesar 0,98 dan 2,85 mM, 0,95 dan 2,36 µM, serta 0,98 dan 1,36 µM. Validasi dengan ekstrak daun sawit sehat menunjukkan kemampuan SPE untuk deteksi phytol sesuai dengan penelitian sebelumnya. Namun, SPE belum dapat menguantifikasi konsentrasi senyawa quinoline dengan sampel ekstrak daun sawit karena interferensi dari senyawa lain.
===============================================================================================================================
Palm oil is extensively produced in Indonesia and Malaysia, accounting for up to 84% of global production. However, there is a potential annual loss of up to 500 million USD due to the Ganoderma boninense infection. Oil palm plantations in Indonesia experience infection spread of up to 45%, necessitating intensive early monitoring to reduce the spread of the infection. The developed method involves electrochemical detection using a screen-printed electrode (SPE) to detect secondary metabolite compounds produced by Ganoderma-infected oil palms, including phytol, quinoline, and stigmasterol. The SPE was fabricated using conductive carbon ink on transparent sticker paper. To enhance the SPE's performance, modifications were made using MWCNT-Ag/Pt and MWCNT-Au/Pt nanocomposites. Ag (5 mM) and Pt (2.5, 5, and 10 mM) nanoparticles were synthesized using a hydrothermal method, while Au (5 mM) was synthesized via citrate reduction. The nanocomposites were formed using sonication and deposited on the SPE through drop-casting. The nanoparticles and nanocomposites were evaluated using UV-vis spectroscopy, FTIR, XPS, and XRD, while the SPE was evaluated using SEM, hydrophobicity test, four-point probe measurements, CV, and EIS. Secondary metabolite detection was conducted using DPV and chronoamperometry in 0.1 M PBS supporting electrolyte. Based on the characterization of the nanoparticles and SPE, the nanocomposites were successfully formed by sonicating the nanoparticles with MWCNT and deposited onto the SPE using the drop-casting method. Electrochemical characterization showed that the best variation for SPE modification was the nanocomposite with a 3:1 ratio and 5 mM Pt concentration, both for MWCNT-Ag/Pt and MWCNT-Au/Pt. Repeated testing on the SPE demonstrated good stability, despite being designed as disposable test kit. The best detection results using DPV were achieved by the MWCNT-Au/Pt-modified SPE for phytol, quinoline, and stigmasterol, with respective R² and LOD values of 0.98 and 2.85 mM, 0.95 and 7.15 µM, and 0.98 and 1.36 µM. Validation using healthy oil palm leaf extract showed the SPE's ability to detect phytol, in line with previous studies. However, the SPE was not yet able to quantify quinoline concentration in the leaf extract sample due to interference from other compounds.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Ganoderma boninense, screen-printed electrode, metabolit sekunder, elektrokimia, biosensor, secondary metabolites, electrochemical |
| Subjects: | Q Science > QD Chemistry > QD115 Electrochemical analysis |
| Divisions: | Faculty of Industrial Technology and Systems Engineering (INDSYS) > Physics Engineering > 30101-(S2) Master Thesis |
| Depositing User: | Arda Fridua Putra |
| Date Deposited: | 30 Jul 2025 04:23 |
| Last Modified: | 30 Jul 2025 04:23 |
| URI: | http://repository.its.ac.id/id/eprint/123798 |
Actions (login required)
 |
View Item |