Pengembangan Hydrogel Berbasis Collagen Type-1/Hydroxyapatite/Chitosan: Studi Rasio Blending Terhadap Sifat Fisik, Kimia, Antibakteri, dan Biokompatibilitas

Fahreza, Naufal Ardhia and Perwitasari, Tri Sita Endah (2025) Pengembangan Hydrogel Berbasis Collagen Type-1/Hydroxyapatite/Chitosan: Studi Rasio Blending Terhadap Sifat Fisik, Kimia, Antibakteri, dan Biokompatibilitas. Other thesis, Institut Teknologi Sepuluh Nopember.

Text 2041211027_2041211055-Undergraduate_Thesis.pdf - Accepted Version Restricted to Repository staff only Download (2MB) | Request a copy

Abstract

Cacat tulang (bone defect) merupakan tantangan utama dalam rekayasa jaringan ortopedi akibat keterbatasan kemampuan regeneratif alami tulang serta tingginya kebutuhan akan material implan yang bersifat bioaktif, biokompatibel, dan mampu berintegrasi dengan jaringan tubuh. Menurut Survei Kesehatan Dasar Indonesia (Riskesdas) 2018, lebih dari 92.000 kasus cedera dilaporkan, dengan 5,5% di antaranya melibatkan cacat tulang. Dalam upaya mengatasi permasalahan tersebut, hydrogel berbasis biomaterial menawarkan solusi inovatif dan potensial melalui kemampuannya yang menjanjikan untuk regenerasi tulang berkat biokompatibilitasnya yang baik dalam mendukung adhesi dan proliferasi sel. Penelitian ini bertujuan untuk mengevaluasi pengaruh variasi rasio blending Collagen Type-1 (COL-1), Hydroxyapatite (HAp), dan Chitosan (CS) terhadap sifat fisik, kimia, antibakteri, dan bikompatibilitas hydrogel untuk aplikasi regenerasi tulang. Hydrogel disintesis dengan metode blending menggunakan glutaraldehyde sebagai agen crosslinking, kemudian dikarakterisasi menggunakan Fourier Transform Infrared Spectroscopy (FTIR), uji compressive strength, dan analisis laju degradasi. Pengujian antibakteri dilakukan terhadap Pseudomonas aeruginosa dan Staphylococcus aureus menggunakan metode zona hambat (Kirby-Bauer), sedangkan uji bioaktivitas dilakukan melalui perendaman dalam larutan Simulated Body Fluid (SBF).
=====================================================================================================================================
Bone defects are a major challenge in orthopedic tissue engineering due to the limitations of natural bone regenerative capabilities and the high demand for bioactive, biocompatible implant materials that can integrate with body tissue. According to the 2018 Indonesian Basic Health Survey (Riskesdas), over 92.000 injury cases were reported, with 5.5% of them involving bone defects. In an effort to address this issue, biomaterial-based hydrogels offer an innovative and promising solution through their potential for bone regeneration, based to their excellent biocompatibility in supporting cell adhesion and proliferation. This study aims to evaluate the effect of varying blending ratios of Collagen Type-1 (COL-1), Hydroxyapatite (HAp), and Chitosan (CS) on the physical, chemical, antibacterial, and biocompatibility properties of hydrogels for bone regeneration applications. The hydrogel was synthesized using the blending method with glutaraldehyde as the crosslinking agent, then characterized using Fourier Transform Infrared Spectroscopy (FTIR), compressive strength testing, and degradation rate analysis. Antibacterial testing was performed against Pseudomonas aeruginosa and Staphylococcus aureus using the zone inhibition method (Kirby-Bauer), while bioactivity testing was conducted through immersion in Simulated Body Fluid (SBF) solution.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Cacat Tulang, Hydrogel Collagen Type-1/Hydroxyapatite/Chitosan, Regenerasi Tulang, Bone Defects, Hydrogel Collagen Type-1/Hydroxyapatite/Chitosan, Bone Regeneration
Subjects:	R Medicine > RD Surgery > RD755.6 Bone substitutes.
Divisions:	Faculty of Vocational > Industrial Chemical Engineering
Depositing User:	Naufal Ardhia Fahreza
Date Deposited:	01 Aug 2025 03:24
Last Modified:	01 Aug 2025 03:24
URI:	http://repository.its.ac.id/id/eprint/124567

Actions (login required)

View Item