Armadan, Sakinah Yaum (2025) Pengaruh Jenis Dan Konsentrasi Crosslinker Terhadap Sifat Mekanik Dan Pelepasan Secretome Pada BHA Dengan Metode Freeze Thaw Untuk Regenerasi Tulang. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Penelitian ini membahas pengaruh jenis dan konsentrasi crosslinker terhadap sifat mekanik serta pelepasan secretome pada biomaterial berbasis bovine hydroxyapatite (BHA) untuk aplikasi regenerasi tulang. BHA memiliki biokompatibilitas tinggi, namun terbatas pada sifat mekaniknya yang rendah. Oleh karena itu, dilakukan modifikasi melalui proses crosslinking dengan menggunakan polyvinyl alcohol (PVA) dan glutaraldehyde (GTA) melalui metode freeze-thaw untuk meningkatkan stabilitas dan ketahanan mekaniknya. Setelah proses crosslinking, BHA direndam dengan secretome yang mengandung protein dari stem cell BM-MSC untuk mendukung penyembuhan dan regenerasi jaringan. Penelitian ini mengevaluasi pengaruh variasi jenis crosslinker yaitu PVA dan GTA dengan konsentrasi (0,1%; 0,5%; 0,7%) terhadap morfologi, karakterisasi, sifat mekanik, degradasi, dan pelepasan secretome selama 14 hari. Pengujian yang dilakukan meliputi FTIR, SEM, XRD, uji tekan, uji degradasi, uji sudut kontak, dan UV-Vis untuk menganalisis pelepasan protein. Hasil penelitian menunjukkan bahwa crosslinker PVA dan GTA dapat meningkatkan sifat mekanik pada BHA hingga 18,07 ± 1,84 MPa, dimana hal tersebut selinear dengan hasil pengujian SEM. Selain itu, pada uji degradasi semua variasi dapat memberikan stabilitas dimana PVA memiliki laju degradasi yang lebih rendah dan GTA mempercepat degradasi. Namun, keduanya tetap menunjukkan stabilitas dengan menunjukkan weight ratio (%) sebesar 89-100%. Kemudian, pelepasan secretome terjadi secara burst release pada hari pertama dan sustained release pada hari ketujuh dan keempat belas. Sehingga, dapat diketahui bahwa semua variasi jenis dan konsentrasi sampel memiliki sifat biocompatibility yang baik.
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This study investigates the effects of crosslinker type and concentration on the mechanical properties and secretome release of bovine hydroxyapatite (BHA)-based biomaterials for bone regeneration applications. BHA has high biocompatibility but is limited by its low mechanical properties. Therefore, modification was carried out through a crosslinking process using polyvinyl alcohol (PVA) and glutaraldehyde (GTA) via a freeze-thaw method to enhance its stability and mechanical strength. After the crosslinking process, BHA was immersed in secretome containing proteins from BM-MSC stem cells to support healing and tissue regeneration. This study evaluates the effects of varying crosslinker types (PVA and GTA) and concentrations (0.1%, 0.5%, 0.7%) on morphology, characterization, mechanical properties, degradation, and secretome release over 14 days. The tests conducted include FTIR, SEM, XRD, compressive strength test, degradation test, contact angle test, and UV-Vis to analyze protein release. The results show that PVA and GTA crosslinkers can enhance the mechanical properties of BHA up to 18.07 ± 1.84 MPa, which is consistent with the SEM test results. Furthermore, all variations in the degradation test showed stability, with PVA having a lower degradation rate and GTA accelerating degradation. However, both still exhibited stability with weight ratio (%) ranging from 89 to 100%. Additionally, secretome release occurred in a burst release pattern on day 1 and sustained release on days 7 and 14. Therefore, it can be concluded that all variations in crosslinker type and concentration exhibited good biocompatibility properties.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Bovine Hydroxyapatite, Crosslink, Glutaraldehyde, Polyvinyl Alcohol, Secretome, Bovine Hydroxyapatite, Crosslink, Glutaraldehyde, Polyvinyl alcohol, Secretome |
| Subjects: | R Medicine > RD Surgery > RD755.5.C35 Orthopedic implants. T Technology > T Technology (General) > TA404 Materials--Biodegradation T Technology > TP Chemical technology > TP156.C64 Controlled release technology |
| Divisions: | Faculty of Industrial Technology and Systems Engineering (INDSYS) > Material & Metallurgical Engineering > 28201-(S1) Undergraduate Thesis |
| Depositing User: | Sakinah Yaum Armadan |
| Date Deposited: | 01 Aug 2025 01:54 |
| Last Modified: | 01 Aug 2025 01:54 |
| URI: | http://repository.its.ac.id/id/eprint/124926 |
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