Fajriah, Amel Apriliani (2025) Keanekaragaman Bakteri dari Serasah Mangrove Wonorejo dengan Pendekatan Taksonomi Filogenetik yang Berpotensi Menghasilkan Polyunsaturated Fatty Acid (PUFA) dan Asam Organik. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Polyunsaturated Fatty Acid (PUFA) merupakan asam lemak esensial yang tidak dapat diproduksi sendiri dalam tubuh manusia dan hanya dapat diperoleh dari asupan makanan seperti ikan, biji-bijian dan mikroorganisme termasuk bakteri. PUFA berperan penting dalam perkembangan sistem saraf, bersifat anti-inflamasi, dan dapat mengatasi gangguan mental. Sebagai asam organik dengan rantai karbon alifatik tak jenuh dan gugus karboksliat, PUFA termasuk dalam kategori senyawa organik yang luas. Pada penelitian sebelumnya telah berhasil mengisolasi enam jenis bakteri pereduksi Triphenyl Tetrazolium Chloride (TTC) dari serasah mangrove Wonorejo sebagai indikator awal penghasil PUFA dan asam organik. Penelitian ini bertujuan untuk mengidentifikasi spesies dari isolat pereduksi TTC berdasarkan analisis sekuens gen 16S rRNA, menentukan hubungan kekerabatan antar isloat bakteri pereduksi TTC dengan menggunakan pendekatan taksonomi filogenetik dan mengetahui isolat pereduksi TTC yang mampu menghasilkan PUFA, dan asam organik melalui uji analisis Gas Choromatography – Mass Spectrophotometry (GC-MS). Hasil yang didapatkan berdasarkan analisis sekuen gen 16S rRNA adalah Prista flexa (SM 1.F), Micrococcus luteus (SM 2A.2), Klebsiella variicola (SM 2.4), Pseudomonas pseudoalcaligenes (SM 2.8), Staphylococcus warneri (SM 2.6) dan Serratia marcescens (RS1. B) dengan hubungan kekerabatan antar isolat memiliki jarak genetik antara 0-1,461. Hasil uji analisis GC-MS yang dilakukan pada bakteri gram negatif tidak menghasilkan PUFA, namun menghasilkan berbagai asam organik seperti asam ftalat, asam sinamat, asam elaidat, asam palmitat, asam stearat, dan asam miristat yang memiliki potensi dalam bidang bioremediasi ftalat, industri farmasi dan industri kosmetik.
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Polyunsaturated Fatty Acid (PUFA) is an essential fatty acid that cannot be produced in the human body and can only be obtained from food intake such as fish, grains and microorganisms including bacteria. PUFA plays an important role in the development of the nervous system, is anti-inflammatory, and can overcome mental disorders. As organic acids with unsaturated apliphatic carbon chains and carboxylic groups, PUFAs are included in the broad category of organic compounds. Previous research has successfully isolated six types of Triphenyl Tetrazolium Chloride (TTC) reducing bacteria from Wonorejo mangrove litter as an early indicator of PUFA and organic acids production. This study aims to identify species of TTC-reducing isolates based on 16S rRNA gene sequence analysis, determine the kinship relationship between TTC-reducing bacterial isolates using a phylogenetic taxonomic approach and determine TTC-reducing isolates that are able to produce PUFAs, and organic acids through Gas Choromatography - Mass Spectrophotometry (GC-MS) analysis test. The results obtained based on 16S rRNA gene sequence analysis are Prista flexa, Micrococcus luteus, Klebsiella variicola, Pseudomonas pseudoalcaligenes, Staphylococcus warneri and Serratia marcescens with kinship relationships between isolates having a genetic distance of 0-1,461. And GC-MS analysis test results conducted on gram-negative bacteria are not able to produce PUFAs, but produce various organic acid such as phtalic acid, cinnamic acid, elaidic acid, palmitic acid, strearic acid, and myristic acid which have potential in the fields of bioremediation, pharmaceutical industries and cosmetic industries.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | 16S rRNA, Asam organik, GC-MS, PUFA. 16s rRNA, Organic acid, GC-MS, PUFA. |
| Subjects: | Q Science > QH Biology Q Science > QH Biology > QH91.8.S64 Species diversity Q Science > QR Microbiology > QR74.8 Bacteria |
| Divisions: | Faculty of Mathematics and Science > Biology > 46201-(S1) Undergraduate Thesis |
| Depositing User: | Amel Apriliani Fajriah |
| Date Deposited: | 01 Aug 2025 02:15 |
| Last Modified: | 01 Aug 2025 02:15 |
| URI: | http://repository.its.ac.id/id/eprint/125607 |
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