Husna, Nisrina Arifatul (2025) Studi Komparatif Metode Ekstraksi, Profil Fitokimia, dan Penambatan Molekular Minyak Atsiri Adas (Foeniculum Vulgare) Sebagai Agen Antibakteri terhadap Staphylococcus Aureus. Other thesis, Institut Teknologi Sepuluh Nopember.
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Abstract
Minyak atsiri yang diperoleh dari biji adas (Foeniculum vulgare) diketahui memiliki aktivitas biologis, salah satunya sebagai antibakteri. Penelitian ini bertujuan untuk membandingkan tiga metode ekstraksi minyak atsiri, yaitu distilasi air atau hydrodistillation (HD), distilasi uap atau steam distillation (SD), dan distilasi air berbantuan gelombang mikro atau microwave-assisted hydrodistillation (MAHD), serta mengevaluasi potensi senyawa mayor sebagai kandidat antibakteri terhadap Staphylococcus aureus. Proses ekstraksi dilakukan pada biji adas kering dan dilanjutkan dengan analisis komposisi kimia menggunakan GC-MS. Aktivitas biologis senyawa dievaluasi melalui prediksi ADMET dan molecular docking dengan enzim DHFR sebagai target reseptor. Hasil penelitian menunjukkan bahwa metode distilasi uap (SD) memberikan rendemen tertinggi sebesar 1,42%, sedangkan MAHD lebih unggul dalam efisiensi waktu ekstraksi. Analisis GC-MS mengidentifikasi trans-anetol dan p-alilanisol sebagai senyawa utama dengan kelimpahan rata-rata masing-masing sebesar 13,75% dan 11,50%. Hasil molecular docking menunjukkan bahwa trans-anetol memiliki afinitas ikatan dan stabilitas interaksi lebih baik terhadap DHFR dibandingkan p-alilanisol, dengan nilai afinitas mendekati ligan referensi. Berdasarkan hasil tersebut, trans-anetol dari minyak atsiri biji F. vulgare berpotensi sebagai agen antibakteri terhadap S. aureus, dan metode ekstraksi berperan penting dalam menentukan rendemen serta kandungan senyawa bioaktif.
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Essential oil derived from fennel (Foeniculum vulgare) seeds is known for its various biological activities, including antibacterial properties. This study aimed to compare three essential oil extraction methods hydrodistillation (HD), steam distillation (SD), and microwave-assisted hydrodistillation (MAHD) and to evaluate the potential of the major essential oil compounds as antibacterial candidates against Staphylococcus aureus. The isolation process was carried out on dried fennel seeds, followed by chemical composition analysis using GC-MS. The biological activity was assessed through ADMET prediction and molecular docking targeting the DHFR enzyme as a protein receptor. The results showed that steam distillation (SD) yielded the highest oil content at 1.42%, while the MAHD method offered advantages in isolation time efficiency. GC-MS analysis identified trans-anethole and p-allyl anisole as the major compounds, with average abundances of 13.75% and 11.50%, respectively. Molecular docking revealed that trans-anethole had better binding affinity and interaction stability with DHFR compared to p-allyl anisole, as it demonstrated an affinity value approaching that of the reference ligand. Based on these findings, trans-anethole from F. vulgare essential oil holds potential as a candidate antibacterial agent against S. aureus, and the choice of extraction method significantly affects both the yield, and the composition of bioactive compounds obtained.
| Item Type: | Thesis (Other) |
|---|---|
| Uncontrolled Keywords: | Foeniculum vulgare, Antibakteri, DHFR, Metode Ekstraksi, Rendemen. Foeniculum vulgare, Antibacterial, DHFR, Extraction method, Yield. |
| Subjects: | Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry Q Science > QD Chemistry > QD416 Essences and essential oils. Q Science > QD Chemistry > QD63 Extraction |
| Divisions: | Faculty of Natural Science > Chemistry > 47201-(S1) Undergraduate Thesis |
| Depositing User: | Nisrina Arifatul Husna |
| Date Deposited: | 05 Aug 2025 00:41 |
| Last Modified: | 05 Aug 2025 00:41 |
| URI: | http://repository.its.ac.id/id/eprint/125968 |
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