Skrining Bakteri Halofilik dari Tambak Garam Osowilangun dengan Bioaktivitas Antibakteri

Abhiramada, Vien Aisyah (2025) Skrining Bakteri Halofilik dari Tambak Garam Osowilangun dengan Bioaktivitas Antibakteri. Other thesis, Institut Teknologi Sepuluh Nopember.

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Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) adalah bakteri Gram positif yang sangat dicari karena resistensinya terhadap hampir semua antibiotik beta-laktam. Penemuan sumber senyawa antibakteri baru didorong oleh krisis resistensi antibiotik saat ini. Salah satu sumbernya adalah mikroorganisme halofilik yang tumbuh di lingkungan ekstrim seperti tambak garam. Bakteri halofilik yang diisolasi dari tambak garam Osowilangun, Surabaya, diidentifikasi, dikarakterisasi, dan dievaluasi untuk aktivitas antibakteri dalam penelitian ini. Empat isolat (TGA-2-P, TGA-2-K, TGA-7-P, dan TGA-7-K) diambil dan diisolasi menggunakan media Nutrient Agar dengan modifikasi 10% natrium klorida. Isolat ini menunjukkan morfologi kokus dan karakter Gram negatif. Dalam uji resistensi antibiotik, tujuh jenis antibiotik (ofloxacin, gentamicin, meropenem, erythromycin, amoxicillin, chloramphenicol, dan doxycycline) menunjukkan profil resistensi yang berbeda; zona hambat Staphylococcus aureus rata-rata adalah 60,26%. Efektivitas antibiotik tertinggi adalah Chloramphenicol (96,15%), sedangkan yang terendah adalah Ofloxacin (32,5%). Menurut uji antagonis, hanya isolat TGA-2-K memiliki sifat antibakteri terhadap S. aureus. Hasil ini menunjukkan bahwa mikroorganisme halofilik di lingkungan tambak garam dapat berfungsi sebagai sumber senyawa antibakteri baru dan opsi pengobatan alternatif untuk patogen yang tidak dapat diobati.
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Methicillin-resistant Staphylococcus aureus (MRSA) is a Gram-positive bacterium that is highly sought after due to its resistance to almost all beta-lactam antibiotics. The discovery of new sources of antibacterial compounds is driven by the current antibiotic resistance crisis. One source is halophilic microorganisms that grow in extreme environments such as salt ponds. Halophilic bacteria isolated from Osowilangun salt ponds, Surabaya, were identified, characterized, and evaluated for antibacterial activity in this study. Four isolates (TGA-2-P, TGA-2-K, TGA-7-P, and TGA-7-K) were taken and isolated using Nutrient Agar media modified with 10% sodium chloride. These isolates showed coccus morphology and Gram-negative characters. In the antibiotic resistance test, seven types of antibiotics (Ofloxacin, Gentamicin, Meropenem, Erythromycin, Amoxicillin, Chloramphenicol, and Doxycycline) showed different resistance profiles; the average inhibition zone of Staphylococcus aureus was 60.26%. The highest antibiotic effectiveness was Chloramphenicol (96.15%), while the lowest was Ofloxacin (32.5%). According to the antagonist test, only isolate TGA-2-K had antibacterial properties against S. aureus. These results indicate that halophilic microorganisms in the salt pond environment can serve as a source o

Item Type:	Thesis (Other)
Uncontrolled Keywords:	Antibiotik, bakteri halofilik, isolasi bakteri, resistensi antibiotik, pewarnaan Gram, zona hambat Antibiotics, halophilic bacteria, bacterial isolation, antibiotic resistance, Gram staining, inhibition zone
Subjects:	Q Science > QD Chemistry > QD251.2 Chemistry, Organic. Biochemistry
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Vien Aisyah Abhiramada
Date Deposited:	04 Aug 2025 04:15
Last Modified:	04 Aug 2025 04:15
URI:	http://repository.its.ac.id/id/eprint/126802

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