Peningkatan Stabilitas Dispersi Senyawa Turunan Imidazol 4-(4,5-difenil-1H-imidazol-2-il)fenol Melalui Modifikasi Permukaan Dengan Gelatin

Putri, Kharisma Amalia Septia (2025) Peningkatan Stabilitas Dispersi Senyawa Turunan Imidazol 4-(4,5-difenil-1H-imidazol-2-il)fenol Melalui Modifikasi Permukaan Dengan Gelatin. Other thesis, Institut Teknologi Sepuluh Nopember.

Text 5004211086_UNDERGRADUATE_Thesis.pdf - Accepted Version Restricted to Repository staff only Download (3MB) | Request a copy

Abstract

Senyawa imidazol dan turunannya, seperti 4-(4,5-difenil-1H-imidazol-2-il)fenol, memiliki potensi sebagai obat anti-kanker. Peningkatan kemampuan anti-kanker senyawa 4-(4,5-difenil-1H-imidazol-2-il)fenol dapat dicapai melalui modifikasi ukurannya ke skala nano dengan metode re-presipitasi. Stabilitas dispersi 4-(4,5-difenil-1H-imidazol-2-il)fenol dalam lingkungan biologis seperti pembuluh darah pada pH 7 dan lingkungan sel kanker pada pH 5 masih menjadi tantangan karena kecenderungannya untuk beragregasi, sehingga diperlukan adanya material modifikasi untuk mencegah agregasi. Penelitian ini bertujuan untuk meningkatkan stabilitas dispersi 4-(4,5-difenil-1H-imidazol-2-il)fenol melalui modifikasi permukaan dengan gelatin yang berasal dari ikan bandeng, dalam lingkungan biologis buatan melalui penambahan media Phosphate Buffer Saline (PBS). Penelitian dilakukan dengan skrining pelarut untuk pembuatan dispersi 4-(4,5-difenil-1H-imidazol-2-il)fenol dengan variasi pelarut yaitu dimetil formamida (DMF), dimetil sulfoksida (DMSO), tetrahidrofuran (THF), dan aseton. Penelitian dilanjutkan dengan skrining dan evaluasi stabilitas dispersi 4-(4,5-difenil-1H-imidazol-2-il)fenol melalui modifikasi permukaan dengan gelatin ikan bandeng pada variasi konsentrasi 2,5; 25; 100; dan 250 μg/mL, serta uji stabilitas pH pada variasi pH 5 dan pH 7. Pengamatan hasil dispersi dilakukan dengan sinar laser melalui efek Tyndall, ukuran partikel yang terbentuk dikarakterisasi dengan Particle Size Analyzer (PSA), dan morfologi yang terbentuk dikarakterisasi dengan Field Emission Scanning Electron Microscope (FE-SEM). Hasil penelitian menunjukkan bahwa pelarut yang paling cocok adalah THF dengan ukuran rata-rata partikel sebesar 239 nm tanpa penambahan PBS dan sebesar 248 nm setelah penambahan PBS, konsentrasi optimal gelatin ikan bandeng adalah sebesar 25 μg/mL dengan ukuran partikel rata-rata sebesar 237 nm dan morfologi tidak seragam. Pada hasil uji stabilitas pH diperoleh hasil bahwa modifikasi permukaan untuk dispersi 4-(4,5-difenil-1H-imidazol-2-il)fenol dengan gelatin ikan bandeng pada konsentrasi 25 μg/mL mampu menurunkan ukuran partikel. Pada media PBS pH 5 dan pH 7, ukuran rata-rata partikel hasil modifikasi masing-masing sebesar 317 nm dan 379 nm, sedangkan tanpa modifikasi, ukurannya jauh lebih besar, yaitu sebesar 971 nm dan 1066 nm.
==========================================================================================================================================
Imidazole compounds and their derivatives, such as 4-(4,5-diphenyl-1H-imidazol-2-yl)phenol, have shown potential as anti-cancer drugs. The anti-cancer activity of 4-(4,5-diphenyl-1H-imidazol-2-yl)phenol can be enhanced by modifying its size to the nanoscale using the re-precipitation method. The stability of 4-(4,5-diphenyl-1H-imidazol-2-yl)phenol dispersion in biological environments such as blood vessels at pH 7 and cancer cell environments at pH 5 remains a challenge due to its tendency to aggregate, thus requiring modified materials to prevent aggregation. This study aims to improve the stability of 4-(4,5-diphenyl-1H-imidazol-2-yl)phenol dispersion through surface modification using gelatin derived from milkfish in an artificial biological environment by adding Phosphate Buffer Saline (PBS) medium. This study was conducted by screening solvents for the preparation of 4-(4,5-diphenyl-1H-imidazol-2-yl)phenol dispersions. The solvents used were dimethylformamide (DMF), dimethyl sulfoxide (DMSO), tetrahydrofuran (THF), and acetone. This study continued with screening and evaluating the stability of the 4-(4,5-diphenyl-1H-imidazol-2-yl)phenol dispersion through surface modification with milkfish gelatin at varying concentrations of 2.5, 25, 100, and 250 μg/mL, as well as pH stability testing at pH 5 and pH 7. The dispersion results were observed using laser light through the Tyndall effect, the particle size was characterized using a Particle Size Analyzer (PSA), and the morphology was characterized using a Field Emission Scanning Electron Microscope (FE-SEM). The results showed that the most suitable solvent was THF, with an average particle size of 239 nm without PBS addition and 248 nm after PBS addition. The optimal concentration of milkfish gelatin was 25 μg/mL, with an average particle size of 237 nm and non-uniform morphology. In the pH stability test results, it was found that surface modification for the dispersion of 4-(4,5-diphenyl-1H-imidazol-2-yl)phenol with milkfish gelatin at a concentration of 25 μg/mL reduced particle size. In PBS medium at pH 5 and pH 7, the average particle size of the modified dispersion was 317 nm and 379 nm, respectively, while without modification, the size was much larger, at 971 nm and 1066 nm.

Item Type:	Thesis (Other)
Uncontrolled Keywords:	4-(4,5-difenil-1H-imidazol-2-il)fenol, Gelatin, Modifikasi Permukaan, Stabilitas, 4-(4,5-diphenyl-1H-imidazol-2-yl)phenol, Gelatin, Stability, Surface Modification
Subjects:	Q Science > QD Chemistry > QD75.2 Chemistry, Analytic
Divisions:	Faculty of Science and Data Analytics (SCIENTICS) > Chemistry > 47201-(S1) Undergraduate Thesis
Depositing User:	Kharisma Amalia Septia Putri
Date Deposited:	05 Aug 2025 01:04
Last Modified:	05 Aug 2025 01:06
URI:	http://repository.its.ac.id/id/eprint/127016

Actions (login required)

View Item